Experiment: monoculture; Experiment B, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00179 and MIT1002_00180 are separated by 344 nucleotides MIT1002_00180 and MIT1002_00181 are separated by 65 nucleotides MIT1002_00181 and MIT1002_00182 are separated by 74 nucleotides
MIT1002_00179: MIT1002_00179 - Excinuclease ABC subunit A, at 193,324 to 196,155
_00179
MIT1002_00180: MIT1002_00180 - Inner membrane transport protein YajR, at 196,500 to 197,837
_00180
MIT1002_00181: MIT1002_00181 - Fructose-1-phosphate phosphatase YqaB, at 197,903 to 198,487
_00181
MIT1002_00182: MIT1002_00182 - hypothetical protein, at 198,562 to 199,155
_00182
Position (kb) 196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11
12 at 195.503 kb on + strand, within MIT1002_00179 at 195.503 kb on + strand, within MIT1002_00179 at 195.513 kb on + strand, within MIT1002_00179 at 195.539 kb on - strand, within MIT1002_00179 at 195.539 kb on - strand, within MIT1002_00179 at 195.539 kb on - strand, within MIT1002_00179 at 195.662 kb on + strand, within MIT1002_00179 at 195.662 kb on + strand, within MIT1002_00179 at 195.662 kb on + strand, within MIT1002_00179 at 195.662 kb on + strand, within MIT1002_00179 at 195.670 kb on - strand, within MIT1002_00179 at 195.670 kb on - strand, within MIT1002_00179 at 195.733 kb on + strand, within MIT1002_00179 at 195.733 kb on + strand, within MIT1002_00179 at 195.741 kb on - strand, within MIT1002_00179 at 195.741 kb on - strand, within MIT1002_00179 at 195.741 kb on - strand, within MIT1002_00179 at 195.789 kb on + strand, within MIT1002_00179 at 195.789 kb on + strand, within MIT1002_00179 at 195.789 kb on + strand, within MIT1002_00179 at 195.789 kb on + strand, within MIT1002_00179 at 195.789 kb on + strand, within MIT1002_00179 at 195.797 kb on - strand, within MIT1002_00179 at 195.797 kb on - strand, within MIT1002_00179 at 195.797 kb on - strand, within MIT1002_00179 at 195.797 kb on - strand, within MIT1002_00179 at 195.797 kb on - strand, within MIT1002_00179 at 195.869 kb on + strand, within MIT1002_00179 at 195.869 kb on + strand, within MIT1002_00179 at 195.917 kb on + strand at 195.923 kb on + strand at 195.981 kb on - strand at 196.003 kb on + strand at 196.011 kb on - strand at 196.123 kb on + strand at 196.231 kb on - strand at 196.259 kb on + strand at 196.267 kb on - strand at 196.335 kb on - strand at 196.357 kb on + strand at 196.357 kb on + strand at 196.365 kb on - strand at 196.395 kb on + strand at 196.400 kb on + strand at 196.400 kb on + strand at 196.423 kb on - strand at 196.441 kb on + strand at 196.449 kb on - strand at 196.568 kb on + strand at 196.568 kb on + strand at 196.576 kb on - strand at 196.576 kb on - strand at 196.576 kb on - strand at 196.604 kb on + strand at 196.612 kb on - strand at 196.630 kb on + strand at 196.656 kb on + strand, within MIT1002_00180 at 196.657 kb on + strand, within MIT1002_00180 at 196.659 kb on - strand, within MIT1002_00180 at 196.659 kb on - strand, within MIT1002_00180 at 196.661 kb on + strand, within MIT1002_00180 at 196.743 kb on - strand, within MIT1002_00180 at 196.767 kb on - strand, within MIT1002_00180 at 196.789 kb on - strand, within MIT1002_00180 at 196.791 kb on - strand, within MIT1002_00180 at 196.820 kb on - strand, within MIT1002_00180 at 196.835 kb on - strand, within MIT1002_00180 at 196.911 kb on + strand, within MIT1002_00180 at 197.050 kb on + strand, within MIT1002_00180 at 197.050 kb on + strand, within MIT1002_00180 at 197.055 kb on + strand, within MIT1002_00180 at 197.063 kb on - strand, within MIT1002_00180 at 197.105 kb on + strand, within MIT1002_00180 at 197.148 kb on + strand, within MIT1002_00180 at 197.339 kb on - strand, within MIT1002_00180 at 197.365 kb on + strand, within MIT1002_00180 at 197.550 kb on - strand, within MIT1002_00180 at 197.654 kb on - strand, within MIT1002_00180 at 197.657 kb on + strand, within MIT1002_00180 at 197.657 kb on + strand, within MIT1002_00180 at 197.664 kb on - strand, within MIT1002_00180 at 197.664 kb on - strand, within MIT1002_00180 at 197.665 kb on - strand, within MIT1002_00180 at 197.665 kb on - strand, within MIT1002_00180 at 197.692 kb on + strand, within MIT1002_00180 at 197.692 kb on + strand, within MIT1002_00180 at 197.716 kb on - strand at 197.744 kb on - strand at 197.759 kb on - strand at 197.830 kb on + strand at 197.835 kb on - strand at 197.857 kb on + strand at 197.901 kb on + strand at 197.952 kb on - strand at 197.976 kb on + strand, within MIT1002_00181 at 198.042 kb on + strand, within MIT1002_00181 at 198.077 kb on - strand, within MIT1002_00181 at 198.149 kb on + strand, within MIT1002_00181 at 198.158 kb on - strand, within MIT1002_00181 at 198.168 kb on + strand, within MIT1002_00181 at 198.208 kb on - strand, within MIT1002_00181 at 198.236 kb on + strand, within MIT1002_00181 at 198.304 kb on + strand, within MIT1002_00181 at 198.304 kb on + strand, within MIT1002_00181 at 198.304 kb on + strand, within MIT1002_00181 at 198.304 kb on + strand, within MIT1002_00181 at 198.304 kb on + strand, within MIT1002_00181 at 198.304 kb on + strand, within MIT1002_00181 at 198.312 kb on - strand, within MIT1002_00181 at 198.312 kb on - strand, within MIT1002_00181 at 198.312 kb on - strand, within MIT1002_00181 at 198.312 kb on - strand, within MIT1002_00181 at 198.312 kb on - strand, within MIT1002_00181 at 198.312 kb on - strand, within MIT1002_00181 at 198.328 kb on + strand, within MIT1002_00181 at 198.328 kb on + strand, within MIT1002_00181 at 198.328 kb on + strand, within MIT1002_00181 at 198.336 kb on - strand, within MIT1002_00181 at 198.336 kb on - strand, within MIT1002_00181 at 198.336 kb on - strand, within MIT1002_00181 at 198.340 kb on + strand, within MIT1002_00181 at 198.356 kb on - strand, within MIT1002_00181 at 198.373 kb on - strand, within MIT1002_00181 at 198.375 kb on + strand, within MIT1002_00181 at 198.383 kb on - strand, within MIT1002_00181 at 198.396 kb on - strand, within MIT1002_00181 at 198.407 kb on - strand, within MIT1002_00181 at 198.451 kb on - strand at 198.488 kb on + strand at 198.496 kb on - strand at 198.496 kb on - strand at 198.498 kb on + strand at 198.526 kb on - strand at 198.567 kb on - strand at 198.567 kb on - strand at 198.568 kb on - strand at 198.583 kb on - strand at 198.681 kb on + strand, within MIT1002_00182 at 198.705 kb on + strand, within MIT1002_00182 at 198.728 kb on + strand, within MIT1002_00182 at 198.730 kb on + strand, within MIT1002_00182 at 198.745 kb on - strand, within MIT1002_00182 at 198.745 kb on - strand, within MIT1002_00182 at 198.761 kb on + strand, within MIT1002_00182 at 198.769 kb on - strand, within MIT1002_00182 at 198.769 kb on - strand, within MIT1002_00182 at 198.781 kb on - strand, within MIT1002_00182 at 198.781 kb on - strand, within MIT1002_00182 at 198.829 kb on + strand, within MIT1002_00182 at 198.837 kb on - strand, within MIT1002_00182 at 198.837 kb on - strand, within MIT1002_00182
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 6 remove 195,503 + MIT1002_00179 0.77 -1.9 195,503 + MIT1002_00179 0.77 +0.8 195,513 + MIT1002_00179 0.77 +0.3 195,539 - MIT1002_00179 0.78 +0.4 195,539 - MIT1002_00179 0.78 -2.4 195,539 - MIT1002_00179 0.78 -0.1 195,662 + MIT1002_00179 0.83 -0.7 195,662 + MIT1002_00179 0.83 -1.2 195,662 + MIT1002_00179 0.83 +1.5 195,662 + MIT1002_00179 0.83 -0.9 195,670 - MIT1002_00179 0.83 +0.7 195,670 - MIT1002_00179 0.83 +0.2 195,733 + MIT1002_00179 0.85 +1.2 195,733 + MIT1002_00179 0.85 -0.3 195,741 - MIT1002_00179 0.85 +0.8 195,741 - MIT1002_00179 0.85 +1.7 195,741 - MIT1002_00179 0.85 -1.6 195,789 + MIT1002_00179 0.87 +2.0 195,789 + MIT1002_00179 0.87 -0.4 195,789 + MIT1002_00179 0.87 -0.3 195,789 + MIT1002_00179 0.87 +0.3 195,789 + MIT1002_00179 0.87 -3.5 195,797 - MIT1002_00179 0.87 -1.4 195,797 - MIT1002_00179 0.87 +0.0 195,797 - MIT1002_00179 0.87 -0.2 195,797 - MIT1002_00179 0.87 -0.8 195,797 - MIT1002_00179 0.87 -1.1 195,869 + MIT1002_00179 0.90 +0.5 195,869 + MIT1002_00179 0.90 +0.2 195,917 + +0.2 195,923 + +1.7 195,981 - -1.3 196,003 + +2.0 196,011 - +0.0 196,123 + -1.5 196,231 - +1.0 196,259 + +0.8 196,267 - +3.3 196,335 - +0.8 196,357 + -0.5 196,357 + +1.4 196,365 - +0.4 196,395 + +0.0 196,400 + +3.3 196,400 + -0.6 196,423 - +11.8 196,441 + -2.7 196,449 - -1.2 196,568 + -0.8 196,568 + -2.2 196,576 - +1.7 196,576 - -0.7 196,576 - -0.4 196,604 + -1.1 196,612 - -0.7 196,630 + -0.6 196,656 + MIT1002_00180 0.12 +1.2 196,657 + MIT1002_00180 0.12 -1.2 196,659 - MIT1002_00180 0.12 +1.2 196,659 - MIT1002_00180 0.12 -0.2 196,661 + MIT1002_00180 0.12 -0.4 196,743 - MIT1002_00180 0.18 -1.0 196,767 - MIT1002_00180 0.20 +0.2 196,789 - MIT1002_00180 0.22 -2.3 196,791 - MIT1002_00180 0.22 -0.3 196,820 - MIT1002_00180 0.24 +0.0 196,835 - MIT1002_00180 0.25 -1.3 196,911 + MIT1002_00180 0.31 +1.5 197,050 + MIT1002_00180 0.41 -0.4 197,050 + MIT1002_00180 0.41 +0.7 197,055 + MIT1002_00180 0.41 +2.0 197,063 - MIT1002_00180 0.42 -0.3 197,105 + MIT1002_00180 0.45 +0.3 197,148 + MIT1002_00180 0.48 -0.1 197,339 - MIT1002_00180 0.63 +0.0 197,365 + MIT1002_00180 0.65 -1.1 197,550 - MIT1002_00180 0.78 +1.1 197,654 - MIT1002_00180 0.86 -1.3 197,657 + MIT1002_00180 0.86 +1.0 197,657 + MIT1002_00180 0.86 -1.5 197,664 - MIT1002_00180 0.87 -1.1 197,664 - MIT1002_00180 0.87 -2.2 197,665 - MIT1002_00180 0.87 +0.3 197,665 - MIT1002_00180 0.87 -0.5 197,692 + MIT1002_00180 0.89 +1.7 197,692 + MIT1002_00180 0.89 +0.5 197,716 - -0.4 197,744 - -1.7 197,759 - -1.5 197,830 + -1.7 197,835 - +0.7 197,857 + +0.5 197,901 + -0.2 197,952 - -1.4 197,976 + MIT1002_00181 0.12 +0.2 198,042 + MIT1002_00181 0.24 -0.2 198,077 - MIT1002_00181 0.30 -0.2 198,149 + MIT1002_00181 0.42 +0.0 198,158 - MIT1002_00181 0.44 -0.5 198,168 + MIT1002_00181 0.45 -1.1 198,208 - MIT1002_00181 0.52 -0.4 198,236 + MIT1002_00181 0.57 +0.1 198,304 + MIT1002_00181 0.69 -0.3 198,304 + MIT1002_00181 0.69 -0.7 198,304 + MIT1002_00181 0.69 +0.7 198,304 + MIT1002_00181 0.69 -1.7 198,304 + MIT1002_00181 0.69 +0.8 198,304 + MIT1002_00181 0.69 -0.8 198,312 - MIT1002_00181 0.70 +2.0 198,312 - MIT1002_00181 0.70 +3.5 198,312 - MIT1002_00181 0.70 -1.9 198,312 - MIT1002_00181 0.70 -0.2 198,312 - MIT1002_00181 0.70 -0.9 198,312 - MIT1002_00181 0.70 +2.0 198,328 + MIT1002_00181 0.73 -1.4 198,328 + MIT1002_00181 0.73 -2.3 198,328 + MIT1002_00181 0.73 -0.7 198,336 - MIT1002_00181 0.74 +1.7 198,336 - MIT1002_00181 0.74 -0.4 198,336 - MIT1002_00181 0.74 +1.3 198,340 + MIT1002_00181 0.75 +0.5 198,356 - MIT1002_00181 0.77 +1.4 198,373 - MIT1002_00181 0.80 +1.0 198,375 + MIT1002_00181 0.81 +2.4 198,383 - MIT1002_00181 0.82 -0.9 198,396 - MIT1002_00181 0.84 +1.0 198,407 - MIT1002_00181 0.86 -0.7 198,451 - +1.2 198,488 + +0.0 198,496 - -0.8 198,496 - +0.3 198,498 + +2.3 198,526 - -0.1 198,567 - -2.1 198,567 - -0.5 198,568 - +0.9 198,583 - +0.2 198,681 + MIT1002_00182 0.20 +0.1 198,705 + MIT1002_00182 0.24 +0.5 198,728 + MIT1002_00182 0.28 -0.5 198,730 + MIT1002_00182 0.28 +0.1 198,745 - MIT1002_00182 0.31 +0.1 198,745 - MIT1002_00182 0.31 +0.3 198,761 + MIT1002_00182 0.34 -0.3 198,769 - MIT1002_00182 0.35 +1.1 198,769 - MIT1002_00182 0.35 +0.4 198,781 - MIT1002_00182 0.37 +2.0 198,781 - MIT1002_00182 0.37 -0.2 198,829 + MIT1002_00182 0.45 -0.9 198,837 - MIT1002_00182 0.46 -0.1 198,837 - MIT1002_00182 0.46 +0.8
Or see this region's nucleotide sequence