Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_04226 and MIT1002_04227 are separated by 396 nucleotides MIT1002_04227 and MIT1002_04228 are separated by 83 nucleotides MIT1002_04228 and MIT1002_04229 are separated by 60 nucleotides
MIT1002_04226: MIT1002_04226 - hypothetical protein, at 94,595 to 95,008
_04226
MIT1002_04227: MIT1002_04227 - hypothetical protein, at 95,405 to 96,172
_04227
MIT1002_04228: MIT1002_04228 - Excalibur calcium-binding domain protein, at 96,256 to 96,987
_04228
MIT1002_04229: MIT1002_04229 - N-acyl amino acid synthase, PEP-CTERM/exosortase system-associated, at 97,048 to 97,704
_04229
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.473 kb on + strand at 94.481 kb on - strand at 94.534 kb on + strand at 94.543 kb on - strand at 94.550 kb on + strand at 94.550 kb on + strand at 94.558 kb on - strand at 94.558 kb on - strand at 94.564 kb on - strand at 94.565 kb on + strand at 94.566 kb on - strand at 94.571 kb on + strand at 94.581 kb on + strand at 94.589 kb on - strand at 94.589 kb on - strand at 94.589 kb on - strand at 94.599 kb on - strand at 94.687 kb on + strand, within MIT1002_04226 at 94.687 kb on + strand, within MIT1002_04226 at 94.687 kb on + strand, within MIT1002_04226 at 94.687 kb on + strand, within MIT1002_04226 at 94.687 kb on + strand, within MIT1002_04226 at 94.695 kb on - strand, within MIT1002_04226 at 94.695 kb on - strand, within MIT1002_04226 at 94.695 kb on - strand, within MIT1002_04226 at 94.695 kb on - strand, within MIT1002_04226 at 94.707 kb on - strand, within MIT1002_04226 at 94.723 kb on + strand, within MIT1002_04226 at 94.731 kb on - strand, within MIT1002_04226 at 94.733 kb on + strand, within MIT1002_04226 at 94.746 kb on - strand, within MIT1002_04226 at 94.752 kb on - strand, within MIT1002_04226 at 94.801 kb on + strand, within MIT1002_04226 at 94.801 kb on + strand, within MIT1002_04226 at 94.872 kb on + strand, within MIT1002_04226 at 94.872 kb on + strand, within MIT1002_04226 at 94.872 kb on + strand, within MIT1002_04226 at 94.874 kb on - strand, within MIT1002_04226 at 94.880 kb on - strand, within MIT1002_04226 at 94.935 kb on - strand, within MIT1002_04226 at 94.943 kb on - strand, within MIT1002_04226 at 94.943 kb on - strand, within MIT1002_04226 at 94.945 kb on + strand, within MIT1002_04226 at 94.981 kb on + strand at 94.981 kb on - strand at 94.986 kb on + strand at 94.989 kb on - strand at 94.994 kb on - strand at 94.996 kb on + strand at 95.024 kb on + strand at 95.024 kb on + strand at 95.024 kb on - strand at 95.032 kb on - strand at 95.043 kb on - strand at 95.057 kb on + strand at 95.190 kb on + strand at 95.190 kb on + strand at 95.193 kb on - strand at 95.198 kb on - strand at 95.198 kb on - strand at 95.198 kb on - strand at 95.198 kb on - strand at 95.198 kb on - strand at 95.235 kb on + strand at 95.243 kb on - strand at 95.252 kb on - strand at 95.271 kb on + strand at 95.271 kb on + strand at 95.271 kb on + strand at 95.271 kb on + strand at 95.272 kb on - strand at 95.277 kb on - strand at 95.279 kb on - strand at 95.279 kb on - strand at 95.344 kb on - strand at 95.346 kb on - strand at 95.372 kb on + strand at 95.406 kb on - strand at 95.448 kb on - strand at 95.472 kb on + strand at 95.472 kb on + strand at 95.472 kb on + strand at 95.472 kb on + strand at 95.479 kb on + strand at 95.479 kb on - strand at 95.532 kb on - strand, within MIT1002_04227 at 95.541 kb on + strand, within MIT1002_04227 at 95.545 kb on + strand, within MIT1002_04227 at 95.549 kb on - strand, within MIT1002_04227 at 95.583 kb on - strand, within MIT1002_04227 at 95.583 kb on - strand, within MIT1002_04227 at 95.588 kb on + strand, within MIT1002_04227 at 95.588 kb on + strand, within MIT1002_04227 at 95.588 kb on + strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.596 kb on - strand, within MIT1002_04227 at 95.613 kb on + strand, within MIT1002_04227 at 95.621 kb on + strand, within MIT1002_04227 at 95.637 kb on - strand, within MIT1002_04227 at 95.647 kb on - strand, within MIT1002_04227 at 95.648 kb on - strand, within MIT1002_04227 at 95.668 kb on + strand, within MIT1002_04227 at 95.676 kb on + strand, within MIT1002_04227 at 95.682 kb on - strand, within MIT1002_04227 at 95.682 kb on - strand, within MIT1002_04227 at 95.682 kb on - strand, within MIT1002_04227 at 95.684 kb on - strand, within MIT1002_04227 at 95.684 kb on - strand, within MIT1002_04227 at 95.708 kb on - strand, within MIT1002_04227 at 95.710 kb on - strand, within MIT1002_04227 at 95.710 kb on - strand, within MIT1002_04227 at 95.788 kb on + strand, within MIT1002_04227 at 95.788 kb on + strand, within MIT1002_04227 at 95.788 kb on + strand, within MIT1002_04227 at 95.788 kb on - strand, within MIT1002_04227 at 95.851 kb on + strand, within MIT1002_04227 at 95.884 kb on + strand, within MIT1002_04227 at 95.975 kb on - strand, within MIT1002_04227 at 96.040 kb on + strand, within MIT1002_04227 at 96.040 kb on + strand, within MIT1002_04227 at 96.048 kb on - strand, within MIT1002_04227 at 96.055 kb on + strand, within MIT1002_04227 at 96.061 kb on - strand, within MIT1002_04227 at 96.114 kb on - strand at 96.151 kb on - strand at 96.229 kb on - strand at 96.327 kb on + strand at 96.327 kb on + strand at 96.327 kb on + strand at 96.340 kb on + strand, within MIT1002_04228 at 96.356 kb on + strand, within MIT1002_04228 at 96.390 kb on + strand, within MIT1002_04228 at 96.398 kb on - strand, within MIT1002_04228 at 96.399 kb on + strand, within MIT1002_04228 at 96.437 kb on + strand, within MIT1002_04228 at 96.437 kb on + strand, within MIT1002_04228 at 96.437 kb on + strand, within MIT1002_04228 at 96.437 kb on + strand, within MIT1002_04228 at 96.437 kb on + strand, within MIT1002_04228 at 96.445 kb on - strand, within MIT1002_04228 at 96.445 kb on - strand, within MIT1002_04228 at 96.445 kb on - strand, within MIT1002_04228 at 96.445 kb on - strand, within MIT1002_04228 at 96.445 kb on - strand, within MIT1002_04228 at 96.447 kb on - strand, within MIT1002_04228 at 96.447 kb on - strand, within MIT1002_04228 at 96.473 kb on - strand, within MIT1002_04228 at 96.478 kb on + strand, within MIT1002_04228 at 96.479 kb on + strand, within MIT1002_04228 at 96.479 kb on + strand, within MIT1002_04228 at 96.497 kb on - strand, within MIT1002_04228 at 96.497 kb on - strand, within MIT1002_04228 at 96.554 kb on - strand, within MIT1002_04228 at 96.558 kb on + strand, within MIT1002_04228 at 96.566 kb on - strand, within MIT1002_04228 at 96.620 kb on - strand, within MIT1002_04228 at 96.623 kb on + strand, within MIT1002_04228 at 96.623 kb on + strand, within MIT1002_04228 at 96.623 kb on + strand, within MIT1002_04228 at 96.631 kb on - strand, within MIT1002_04228 at 96.648 kb on + strand, within MIT1002_04228 at 96.660 kb on + strand, within MIT1002_04228 at 96.660 kb on + strand, within MIT1002_04228 at 96.701 kb on - strand, within MIT1002_04228 at 96.701 kb on - strand, within MIT1002_04228 at 96.712 kb on - strand, within MIT1002_04228 at 96.712 kb on - strand, within MIT1002_04228 at 96.719 kb on + strand, within MIT1002_04228 at 96.722 kb on - strand, within MIT1002_04228 at 96.722 kb on - strand, within MIT1002_04228 at 96.728 kb on - strand, within MIT1002_04228 at 96.773 kb on + strand, within MIT1002_04228 at 96.781 kb on - strand, within MIT1002_04228 at 96.781 kb on - strand, within MIT1002_04228 at 96.783 kb on + strand, within MIT1002_04228 at 96.807 kb on - strand, within MIT1002_04228 at 96.816 kb on + strand, within MIT1002_04228 at 96.855 kb on - strand, within MIT1002_04228 at 96.857 kb on + strand, within MIT1002_04228 at 96.859 kb on - strand, within MIT1002_04228 at 96.882 kb on - strand, within MIT1002_04228 at 96.892 kb on + strand, within MIT1002_04228 at 96.906 kb on + strand, within MIT1002_04228 at 96.907 kb on + strand, within MIT1002_04228 at 96.907 kb on + strand, within MIT1002_04228 at 96.912 kb on + strand, within MIT1002_04228 at 96.915 kb on - strand at 96.917 kb on + strand at 96.922 kb on + strand at 96.930 kb on - strand at 96.930 kb on - strand at 96.989 kb on + strand at 96.989 kb on + strand at 96.989 kb on + strand at 96.994 kb on + strand at 96.997 kb on - strand at 96.997 kb on - strand at 96.997 kb on - strand at 97.003 kb on + strand at 97.028 kb on - strand at 97.028 kb on - strand at 97.048 kb on - strand at 97.114 kb on + strand, within MIT1002_04229 at 97.134 kb on + strand, within MIT1002_04229 at 97.146 kb on + strand, within MIT1002_04229 at 97.165 kb on - strand, within MIT1002_04229 at 97.167 kb on - strand, within MIT1002_04229 at 97.169 kb on + strand, within MIT1002_04229
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 94,473 + -0.7 94,481 - -1.9 94,534 + -0.7 94,543 - -0.1 94,550 + -0.1 94,550 + +0.3 94,558 - +1.2 94,558 - -2.1 94,564 - +0.2 94,565 + -2.8 94,566 - +0.8 94,571 + +0.3 94,581 + -0.0 94,589 - -1.2 94,589 - -0.9 94,589 - +0.9 94,599 - +1.4 94,687 + MIT1002_04226 0.22 +0.7 94,687 + MIT1002_04226 0.22 -0.3 94,687 + MIT1002_04226 0.22 -0.6 94,687 + MIT1002_04226 0.22 -0.8 94,687 + MIT1002_04226 0.22 +0.2 94,695 - MIT1002_04226 0.24 +1.0 94,695 - MIT1002_04226 0.24 +0.4 94,695 - MIT1002_04226 0.24 +0.3 94,695 - MIT1002_04226 0.24 -0.5 94,707 - MIT1002_04226 0.27 +0.6 94,723 + MIT1002_04226 0.31 -0.4 94,731 - MIT1002_04226 0.33 +0.5 94,733 + MIT1002_04226 0.33 -0.6 94,746 - MIT1002_04226 0.36 +0.6 94,752 - MIT1002_04226 0.38 +0.2 94,801 + MIT1002_04226 0.50 -1.3 94,801 + MIT1002_04226 0.50 +1.0 94,872 + MIT1002_04226 0.67 -0.7 94,872 + MIT1002_04226 0.67 -0.5 94,872 + MIT1002_04226 0.67 -0.8 94,874 - MIT1002_04226 0.67 -1.6 94,880 - MIT1002_04226 0.69 -0.6 94,935 - MIT1002_04226 0.82 -0.4 94,943 - MIT1002_04226 0.84 -0.3 94,943 - MIT1002_04226 0.84 -0.7 94,945 + MIT1002_04226 0.85 +0.8 94,981 + -0.5 94,981 - -1.2 94,986 + +0.4 94,989 - +0.8 94,994 - -0.0 94,996 + +0.2 95,024 + -0.6 95,024 + +1.8 95,024 - -0.1 95,032 - +0.0 95,043 - +0.3 95,057 + -0.1 95,190 + -1.5 95,190 + +0.1 95,193 - -0.5 95,198 - +0.8 95,198 - +1.3 95,198 - -0.5 95,198 - -0.8 95,198 - +0.9 95,235 + -0.4 95,243 - +0.7 95,252 - +0.9 95,271 + -0.4 95,271 + +0.9 95,271 + -1.2 95,271 + +0.3 95,272 - -0.6 95,277 - -0.7 95,279 - -1.2 95,279 - -0.7 95,344 - -0.4 95,346 - -0.5 95,372 + -0.4 95,406 - +1.3 95,448 - +0.9 95,472 + +1.5 95,472 + +0.7 95,472 + -0.7 95,472 + +0.9 95,479 + +0.8 95,479 - +0.0 95,532 - MIT1002_04227 0.17 +0.3 95,541 + MIT1002_04227 0.18 -0.5 95,545 + MIT1002_04227 0.18 -1.6 95,549 - MIT1002_04227 0.19 +1.7 95,583 - MIT1002_04227 0.23 +1.9 95,583 - MIT1002_04227 0.23 -3.7 95,588 + MIT1002_04227 0.24 +0.8 95,588 + MIT1002_04227 0.24 -0.3 95,588 + MIT1002_04227 0.24 +0.3 95,596 - MIT1002_04227 0.25 -1.0 95,596 - MIT1002_04227 0.25 -0.8 95,596 - MIT1002_04227 0.25 +1.6 95,596 - MIT1002_04227 0.25 -0.6 95,596 - MIT1002_04227 0.25 +0.3 95,596 - MIT1002_04227 0.25 +0.6 95,596 - MIT1002_04227 0.25 +0.5 95,596 - MIT1002_04227 0.25 +0.9 95,596 - MIT1002_04227 0.25 +0.3 95,613 + MIT1002_04227 0.27 +0.5 95,621 + MIT1002_04227 0.28 -2.2 95,637 - MIT1002_04227 0.30 +0.7 95,647 - MIT1002_04227 0.32 -0.3 95,648 - MIT1002_04227 0.32 +0.0 95,668 + MIT1002_04227 0.34 +1.9 95,676 + MIT1002_04227 0.35 +0.4 95,682 - MIT1002_04227 0.36 +1.2 95,682 - MIT1002_04227 0.36 -1.1 95,682 - MIT1002_04227 0.36 +0.0 95,684 - MIT1002_04227 0.36 -0.7 95,684 - MIT1002_04227 0.36 +0.8 95,708 - MIT1002_04227 0.39 -1.0 95,710 - MIT1002_04227 0.40 -0.6 95,710 - MIT1002_04227 0.40 +0.1 95,788 + MIT1002_04227 0.50 -0.5 95,788 + MIT1002_04227 0.50 -0.7 95,788 + MIT1002_04227 0.50 +0.6 95,788 - MIT1002_04227 0.50 -0.7 95,851 + MIT1002_04227 0.58 +1.2 95,884 + MIT1002_04227 0.62 -1.7 95,975 - MIT1002_04227 0.74 +0.3 96,040 + MIT1002_04227 0.83 +0.4 96,040 + MIT1002_04227 0.83 -1.1 96,048 - MIT1002_04227 0.84 -0.1 96,055 + MIT1002_04227 0.85 +1.4 96,061 - MIT1002_04227 0.85 -1.7 96,114 - -1.9 96,151 - -0.4 96,229 - -0.2 96,327 + +0.6 96,327 + +0.1 96,327 + +1.2 96,340 + MIT1002_04228 0.11 -0.7 96,356 + MIT1002_04228 0.14 -0.0 96,390 + MIT1002_04228 0.18 -0.9 96,398 - MIT1002_04228 0.19 +1.3 96,399 + MIT1002_04228 0.20 +1.0 96,437 + MIT1002_04228 0.25 +1.9 96,437 + MIT1002_04228 0.25 +1.9 96,437 + MIT1002_04228 0.25 -1.3 96,437 + MIT1002_04228 0.25 -0.9 96,437 + MIT1002_04228 0.25 -1.1 96,445 - MIT1002_04228 0.26 -0.2 96,445 - MIT1002_04228 0.26 -0.2 96,445 - MIT1002_04228 0.26 +0.1 96,445 - MIT1002_04228 0.26 +0.6 96,445 - MIT1002_04228 0.26 -1.5 96,447 - MIT1002_04228 0.26 -0.8 96,447 - MIT1002_04228 0.26 -0.9 96,473 - MIT1002_04228 0.30 -2.2 96,478 + MIT1002_04228 0.30 +0.3 96,479 + MIT1002_04228 0.30 -0.0 96,479 + MIT1002_04228 0.30 -1.4 96,497 - MIT1002_04228 0.33 +0.1 96,497 - MIT1002_04228 0.33 -0.7 96,554 - MIT1002_04228 0.41 -2.0 96,558 + MIT1002_04228 0.41 +0.5 96,566 - MIT1002_04228 0.42 +0.1 96,620 - MIT1002_04228 0.50 -0.5 96,623 + MIT1002_04228 0.50 +1.2 96,623 + MIT1002_04228 0.50 +1.7 96,623 + MIT1002_04228 0.50 -0.3 96,631 - MIT1002_04228 0.51 -0.7 96,648 + MIT1002_04228 0.54 -0.7 96,660 + MIT1002_04228 0.55 -0.2 96,660 + MIT1002_04228 0.55 +0.7 96,701 - MIT1002_04228 0.61 -0.2 96,701 - MIT1002_04228 0.61 -1.6 96,712 - MIT1002_04228 0.62 -1.9 96,712 - MIT1002_04228 0.62 -1.8 96,719 + MIT1002_04228 0.63 -2.2 96,722 - MIT1002_04228 0.64 -0.2 96,722 - MIT1002_04228 0.64 +0.9 96,728 - MIT1002_04228 0.64 +1.2 96,773 + MIT1002_04228 0.71 -0.1 96,781 - MIT1002_04228 0.72 -0.7 96,781 - MIT1002_04228 0.72 -0.9 96,783 + MIT1002_04228 0.72 -0.4 96,807 - MIT1002_04228 0.75 -2.9 96,816 + MIT1002_04228 0.77 +1.1 96,855 - MIT1002_04228 0.82 +0.3 96,857 + MIT1002_04228 0.82 +0.2 96,859 - MIT1002_04228 0.82 +0.6 96,882 - MIT1002_04228 0.86 -1.3 96,892 + MIT1002_04228 0.87 -1.0 96,906 + MIT1002_04228 0.89 +0.3 96,907 + MIT1002_04228 0.89 -0.9 96,907 + MIT1002_04228 0.89 -0.5 96,912 + MIT1002_04228 0.90 -0.5 96,915 - -0.9 96,917 + +0.9 96,922 + -0.5 96,930 - +1.1 96,930 - -1.2 96,989 + -0.1 96,989 + -1.6 96,989 + -0.6 96,994 + -2.2 96,997 - +0.6 96,997 - +0.1 96,997 - +0.4 97,003 + -0.3 97,028 - -0.6 97,028 - -0.1 97,048 - +0.6 97,114 + MIT1002_04229 0.10 -0.7 97,134 + MIT1002_04229 0.13 +0.1 97,146 + MIT1002_04229 0.15 -0.3 97,165 - MIT1002_04229 0.18 +1.3 97,167 - MIT1002_04229 0.18 +0.6 97,169 + MIT1002_04229 0.18 +1.1
Or see this region's nucleotide sequence