Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00036 and MIT1002_00037 are separated by 140 nucleotides MIT1002_00037 and MIT1002_00038 are separated by 136 nucleotides MIT1002_00038 and MIT1002_00039 overlap by 20 nucleotides MIT1002_00039 and MIT1002_00040 are separated by 142 nucleotides
MIT1002_00036: MIT1002_00036 - Transposase, at 40,196 to 40,711
_00036
MIT1002_00037: MIT1002_00037 - Lipid A 3-O-deacylase (PagL), at 40,852 to 41,382
_00037
MIT1002_00038: MIT1002_00038 - hypothetical protein, at 41,519 to 42,349
_00038
MIT1002_00039: MIT1002_00039 - hypothetical protein, at 42,330 to 43,121
_00039
MIT1002_00040: MIT1002_00040 - Error-prone DNA polymerase, at 43,264 to 46,353
_00040
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 40.802 kb on + strand at 40.810 kb on - strand at 40.865 kb on + strand at 40.873 kb on - strand at 40.873 kb on - strand at 40.884 kb on + strand at 40.977 kb on + strand, within MIT1002_00037 at 41.002 kb on + strand, within MIT1002_00037 at 41.051 kb on + strand, within MIT1002_00037 at 41.104 kb on - strand, within MIT1002_00037 at 41.147 kb on - strand, within MIT1002_00037 at 41.210 kb on - strand, within MIT1002_00037 at 41.233 kb on - strand, within MIT1002_00037 at 41.481 kb on + strand at 41.509 kb on + strand at 41.570 kb on - strand at 41.652 kb on - strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.714 kb on + strand, within MIT1002_00038 at 41.722 kb on + strand, within MIT1002_00038 at 41.723 kb on + strand, within MIT1002_00038 at 41.723 kb on + strand, within MIT1002_00038 at 41.731 kb on - strand, within MIT1002_00038 at 41.731 kb on - strand, within MIT1002_00038 at 41.747 kb on + strand, within MIT1002_00038 at 41.767 kb on - strand, within MIT1002_00038 at 41.781 kb on + strand, within MIT1002_00038 at 41.802 kb on - strand, within MIT1002_00038 at 41.827 kb on + strand, within MIT1002_00038 at 41.870 kb on - strand, within MIT1002_00038 at 42.017 kb on - strand, within MIT1002_00038 at 42.026 kb on - strand, within MIT1002_00038 at 42.040 kb on + strand, within MIT1002_00038 at 42.099 kb on + strand, within MIT1002_00038 at 42.107 kb on + strand, within MIT1002_00038 at 42.174 kb on + strand, within MIT1002_00038 at 42.174 kb on + strand, within MIT1002_00038 at 42.177 kb on - strand, within MIT1002_00038 at 42.187 kb on - strand, within MIT1002_00038 at 42.187 kb on - strand, within MIT1002_00038 at 42.187 kb on - strand, within MIT1002_00038 at 42.249 kb on - strand, within MIT1002_00038 at 42.254 kb on + strand, within MIT1002_00038 at 42.299 kb on + strand at 42.367 kb on + strand at 42.392 kb on + strand at 42.397 kb on - strand at 42.561 kb on + strand, within MIT1002_00039 at 42.574 kb on - strand, within MIT1002_00039 at 42.586 kb on + strand, within MIT1002_00039 at 42.608 kb on + strand, within MIT1002_00039 at 42.609 kb on + strand, within MIT1002_00039 at 42.633 kb on + strand, within MIT1002_00039 at 42.633 kb on + strand, within MIT1002_00039 at 42.633 kb on + strand, within MIT1002_00039 at 42.647 kb on - strand, within MIT1002_00039 at 42.718 kb on - strand, within MIT1002_00039 at 42.725 kb on - strand, within MIT1002_00039 at 42.773 kb on - strand, within MIT1002_00039 at 42.791 kb on + strand, within MIT1002_00039 at 42.801 kb on + strand, within MIT1002_00039 at 42.801 kb on + strand, within MIT1002_00039 at 42.801 kb on + strand, within MIT1002_00039 at 42.801 kb on + strand, within MIT1002_00039 at 42.801 kb on + strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.809 kb on - strand, within MIT1002_00039 at 42.846 kb on + strand, within MIT1002_00039 at 42.889 kb on + strand, within MIT1002_00039 at 42.943 kb on - strand, within MIT1002_00039 at 43.103 kb on + strand at 43.103 kb on + strand at 43.103 kb on + strand at 43.111 kb on - strand at 43.111 kb on - strand at 43.120 kb on + strand at 43.128 kb on - strand at 43.128 kb on - strand at 43.222 kb on + strand at 43.230 kb on - strand at 43.255 kb on + strand at 43.308 kb on - strand at 43.309 kb on - strand at 43.316 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 40,802 + -0.3 40,810 - +0.9 40,865 + -0.2 40,873 - -1.3 40,873 - +1.4 40,884 + +1.4 40,977 + MIT1002_00037 0.24 +0.9 41,002 + MIT1002_00037 0.28 -1.0 41,051 + MIT1002_00037 0.37 +1.5 41,104 - MIT1002_00037 0.47 +0.8 41,147 - MIT1002_00037 0.56 -1.1 41,210 - MIT1002_00037 0.67 -0.1 41,233 - MIT1002_00037 0.72 +0.8 41,481 + -0.4 41,509 + -0.4 41,570 - +1.1 41,652 - MIT1002_00038 0.16 +0.6 41,680 + MIT1002_00038 0.19 +0.1 41,680 + MIT1002_00038 0.19 -0.2 41,680 + MIT1002_00038 0.19 +1.0 41,680 + MIT1002_00038 0.19 -0.4 41,680 + MIT1002_00038 0.19 +0.3 41,680 + MIT1002_00038 0.19 +0.0 41,680 + MIT1002_00038 0.19 +0.9 41,680 + MIT1002_00038 0.19 -0.6 41,680 + MIT1002_00038 0.19 +2.2 41,680 + MIT1002_00038 0.19 -0.6 41,688 - MIT1002_00038 0.20 -2.2 41,688 - MIT1002_00038 0.20 -1.0 41,688 - MIT1002_00038 0.20 -0.9 41,688 - MIT1002_00038 0.20 -1.1 41,688 - MIT1002_00038 0.20 -0.4 41,688 - MIT1002_00038 0.20 -0.8 41,714 + MIT1002_00038 0.23 +1.1 41,722 + MIT1002_00038 0.24 -0.3 41,723 + MIT1002_00038 0.25 -0.3 41,723 + MIT1002_00038 0.25 -0.1 41,731 - MIT1002_00038 0.26 +0.1 41,731 - MIT1002_00038 0.26 -2.9 41,747 + MIT1002_00038 0.27 -0.7 41,767 - MIT1002_00038 0.30 -1.4 41,781 + MIT1002_00038 0.32 -2.0 41,802 - MIT1002_00038 0.34 +1.3 41,827 + MIT1002_00038 0.37 +0.0 41,870 - MIT1002_00038 0.42 +0.7 42,017 - MIT1002_00038 0.60 -0.3 42,026 - MIT1002_00038 0.61 +1.4 42,040 + MIT1002_00038 0.63 -1.1 42,099 + MIT1002_00038 0.70 -0.9 42,107 + MIT1002_00038 0.71 +0.1 42,174 + MIT1002_00038 0.79 +1.6 42,174 + MIT1002_00038 0.79 -0.6 42,177 - MIT1002_00038 0.79 +0.5 42,187 - MIT1002_00038 0.80 +0.2 42,187 - MIT1002_00038 0.80 -0.8 42,187 - MIT1002_00038 0.80 -0.6 42,249 - MIT1002_00038 0.88 +0.2 42,254 + MIT1002_00038 0.88 -1.6 42,299 + -1.1 42,367 + +1.7 42,392 + +0.7 42,397 - -0.5 42,561 + MIT1002_00039 0.29 -0.2 42,574 - MIT1002_00039 0.31 +0.3 42,586 + MIT1002_00039 0.32 -1.3 42,608 + MIT1002_00039 0.35 -0.1 42,609 + MIT1002_00039 0.35 +0.8 42,633 + MIT1002_00039 0.38 -0.1 42,633 + MIT1002_00039 0.38 +0.1 42,633 + MIT1002_00039 0.38 +1.1 42,647 - MIT1002_00039 0.40 -0.0 42,718 - MIT1002_00039 0.49 +1.0 42,725 - MIT1002_00039 0.50 -0.2 42,773 - MIT1002_00039 0.56 -0.0 42,791 + MIT1002_00039 0.58 +2.7 42,801 + MIT1002_00039 0.59 +1.4 42,801 + MIT1002_00039 0.59 -0.6 42,801 + MIT1002_00039 0.59 -0.0 42,801 + MIT1002_00039 0.59 +1.6 42,801 + MIT1002_00039 0.59 -0.2 42,809 - MIT1002_00039 0.60 -1.0 42,809 - MIT1002_00039 0.60 +1.1 42,809 - MIT1002_00039 0.60 +0.1 42,809 - MIT1002_00039 0.60 +1.6 42,809 - MIT1002_00039 0.60 -0.8 42,809 - MIT1002_00039 0.60 -0.9 42,809 - MIT1002_00039 0.60 +1.2 42,846 + MIT1002_00039 0.65 +0.2 42,889 + MIT1002_00039 0.71 +0.7 42,943 - MIT1002_00039 0.77 -1.5 43,103 + +0.0 43,103 + -1.8 43,103 + +1.4 43,111 - +0.3 43,111 - +0.2 43,120 + +0.2 43,128 - +1.4 43,128 - -0.5 43,222 + -2.0 43,230 - -0.2 43,255 + +0.4 43,308 - +1.0 43,309 - -0.5 43,316 + -1.8
Or see this region's nucleotide sequence