Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03116 and MIT1002_03117 are separated by 178 nucleotides MIT1002_03117 and MIT1002_03118 are separated by 23 nucleotides MIT1002_03118 and MIT1002_03119 are separated by 40 nucleotides
MIT1002_03116: MIT1002_03116 - Spore coat protein SA, at 3,470,624 to 3,471,712
_03116
MIT1002_03117: MIT1002_03117 - DnaA initiator-associating protein DiaA, at 3,471,891 to 3,472,487
_03117
MIT1002_03118: MIT1002_03118 - Osmotically-inducible protein Y precursor, at 3,472,511 to 3,473,086
_03118
MIT1002_03119: MIT1002_03119 - High-copy suppressor of rspA, at 3,473,127 to 3,474,488
_03119
Position (kb)
3472
3473
3474 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3471.525 kb on - strand, within MIT1002_03116 at 3471.584 kb on + strand, within MIT1002_03116 at 3471.617 kb on + strand at 3471.622 kb on + strand at 3471.704 kb on + strand at 3471.710 kb on - strand at 3471.778 kb on + strand at 3471.778 kb on + strand at 3471.786 kb on - strand at 3471.786 kb on - strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.834 kb on - strand at 3471.834 kb on - strand at 3471.834 kb on - strand at 3471.900 kb on - strand at 3471.972 kb on - strand, within MIT1002_03117 at 3471.991 kb on + strand, within MIT1002_03117 at 3471.999 kb on + strand, within MIT1002_03117 at 3472.020 kb on + strand, within MIT1002_03117 at 3472.138 kb on + strand, within MIT1002_03117 at 3472.143 kb on + strand, within MIT1002_03117 at 3472.143 kb on + strand, within MIT1002_03117 at 3472.146 kb on - strand, within MIT1002_03117 at 3472.229 kb on - strand, within MIT1002_03117 at 3472.262 kb on + strand, within MIT1002_03117 at 3472.270 kb on - strand, within MIT1002_03117 at 3472.566 kb on + strand at 3472.566 kb on + strand at 3472.566 kb on + strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.623 kb on + strand, within MIT1002_03118 at 3472.631 kb on - strand, within MIT1002_03118 at 3472.724 kb on - strand, within MIT1002_03118 at 3472.730 kb on + strand, within MIT1002_03118 at 3472.740 kb on + strand, within MIT1002_03118 at 3472.748 kb on - strand, within MIT1002_03118 at 3472.753 kb on - strand, within MIT1002_03118 at 3472.775 kb on + strand, within MIT1002_03118 at 3472.790 kb on + strand, within MIT1002_03118 at 3472.811 kb on - strand, within MIT1002_03118 at 3472.863 kb on + strand, within MIT1002_03118 at 3472.863 kb on + strand, within MIT1002_03118 at 3472.893 kb on - strand, within MIT1002_03118 at 3472.948 kb on + strand, within MIT1002_03118 at 3472.948 kb on + strand, within MIT1002_03118 at 3472.948 kb on + strand, within MIT1002_03118 at 3473.068 kb on - strand at 3473.088 kb on - strand at 3473.098 kb on - strand at 3473.098 kb on - strand at 3473.197 kb on + strand at 3473.334 kb on + strand, within MIT1002_03119 at 3473.350 kb on + strand, within MIT1002_03119 at 3473.399 kb on - strand, within MIT1002_03119 at 3473.399 kb on - strand, within MIT1002_03119 at 3473.433 kb on - strand, within MIT1002_03119 at 3473.483 kb on + strand, within MIT1002_03119 at 3473.501 kb on - strand, within MIT1002_03119 at 3473.501 kb on - strand, within MIT1002_03119 at 3473.516 kb on - strand, within MIT1002_03119 at 3473.523 kb on - strand, within MIT1002_03119 at 3473.528 kb on - strand, within MIT1002_03119 at 3473.528 kb on - strand, within MIT1002_03119 at 3473.585 kb on + strand, within MIT1002_03119 at 3473.593 kb on - strand, within MIT1002_03119 at 3473.593 kb on - strand, within MIT1002_03119 at 3473.600 kb on + strand, within MIT1002_03119 at 3473.618 kb on - strand, within MIT1002_03119 at 3473.775 kb on - strand, within MIT1002_03119 at 3473.857 kb on + strand, within MIT1002_03119 at 3473.857 kb on + strand, within MIT1002_03119 at 3473.865 kb on - strand, within MIT1002_03119 at 3473.865 kb on - strand, within MIT1002_03119 at 3473.895 kb on - strand, within MIT1002_03119 at 3473.933 kb on + strand, within MIT1002_03119 at 3473.955 kb on + strand, within MIT1002_03119 at 3473.970 kb on + strand, within MIT1002_03119 at 3473.978 kb on - strand, within MIT1002_03119 at 3474.072 kb on + strand, within MIT1002_03119
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 3,471,525 - MIT1002_03116 0.83 +1.8 3,471,584 + MIT1002_03116 0.88 +4.0 3,471,617 + +2.9 3,471,622 + +3.2 3,471,704 + -0.9 3,471,710 - +0.3 3,471,778 + -0.5 3,471,778 + +0.8 3,471,786 - -0.4 3,471,786 - +0.6 3,471,826 + -1.5 3,471,826 + +0.5 3,471,826 + -0.1 3,471,826 + +0.9 3,471,826 + -0.8 3,471,834 - -0.3 3,471,834 - -1.0 3,471,834 - +0.2 3,471,900 - +0.5 3,471,972 - MIT1002_03117 0.14 -0.9 3,471,991 + MIT1002_03117 0.17 -0.9 3,471,999 + MIT1002_03117 0.18 -1.9 3,472,020 + MIT1002_03117 0.22 -0.9 3,472,138 + MIT1002_03117 0.41 -3.3 3,472,143 + MIT1002_03117 0.42 -0.5 3,472,143 + MIT1002_03117 0.42 +0.2 3,472,146 - MIT1002_03117 0.43 -0.7 3,472,229 - MIT1002_03117 0.57 -1.7 3,472,262 + MIT1002_03117 0.62 -1.2 3,472,270 - MIT1002_03117 0.63 -1.2 3,472,566 + -1.3 3,472,566 + -1.1 3,472,566 + -0.9 3,472,567 - -0.4 3,472,567 - -0.5 3,472,567 - -0.4 3,472,567 - +0.0 3,472,567 - -1.5 3,472,623 + MIT1002_03118 0.19 +0.8 3,472,631 - MIT1002_03118 0.21 -0.4 3,472,724 - MIT1002_03118 0.37 +0.2 3,472,730 + MIT1002_03118 0.38 -1.0 3,472,740 + MIT1002_03118 0.40 -1.8 3,472,748 - MIT1002_03118 0.41 -0.8 3,472,753 - MIT1002_03118 0.42 -1.3 3,472,775 + MIT1002_03118 0.46 -0.9 3,472,790 + MIT1002_03118 0.48 -1.6 3,472,811 - MIT1002_03118 0.52 -1.1 3,472,863 + MIT1002_03118 0.61 +0.4 3,472,863 + MIT1002_03118 0.61 -0.7 3,472,893 - MIT1002_03118 0.66 -0.9 3,472,948 + MIT1002_03118 0.76 -1.4 3,472,948 + MIT1002_03118 0.76 +0.3 3,472,948 + MIT1002_03118 0.76 -0.2 3,473,068 - -0.2 3,473,088 - -0.6 3,473,098 - -0.8 3,473,098 - -2.6 3,473,197 + -0.7 3,473,334 + MIT1002_03119 0.15 -1.0 3,473,350 + MIT1002_03119 0.16 +0.5 3,473,399 - MIT1002_03119 0.20 +3.8 3,473,399 - MIT1002_03119 0.20 +0.1 3,473,433 - MIT1002_03119 0.22 -0.5 3,473,483 + MIT1002_03119 0.26 +1.5 3,473,501 - MIT1002_03119 0.27 +0.9 3,473,501 - MIT1002_03119 0.27 +0.9 3,473,516 - MIT1002_03119 0.29 +1.4 3,473,523 - MIT1002_03119 0.29 -0.2 3,473,528 - MIT1002_03119 0.29 +0.4 3,473,528 - MIT1002_03119 0.29 +0.5 3,473,585 + MIT1002_03119 0.34 -2.9 3,473,593 - MIT1002_03119 0.34 -1.7 3,473,593 - MIT1002_03119 0.34 -1.2 3,473,600 + MIT1002_03119 0.35 +1.8 3,473,618 - MIT1002_03119 0.36 +1.1 3,473,775 - MIT1002_03119 0.48 -0.1 3,473,857 + MIT1002_03119 0.54 +1.7 3,473,857 + MIT1002_03119 0.54 +3.6 3,473,865 - MIT1002_03119 0.54 -0.2 3,473,865 - MIT1002_03119 0.54 +0.1 3,473,895 - MIT1002_03119 0.56 -0.6 3,473,933 + MIT1002_03119 0.59 -0.7 3,473,955 + MIT1002_03119 0.61 -0.1 3,473,970 + MIT1002_03119 0.62 -0.8 3,473,978 - MIT1002_03119 0.62 +0.5 3,474,072 + MIT1002_03119 0.69 -0.2
Or see this region's nucleotide sequence