Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02529 and MIT1002_02530 are separated by 204 nucleotides MIT1002_02530 and MIT1002_02531 are separated by 518 nucleotides
MIT1002_02529: MIT1002_02529 - tRNA pseudouridine synthase A, at 2,819,210 to 2,820,001
_02529
MIT1002_02530: MIT1002_02530 - hypothetical protein, at 2,820,206 to 2,825,128
_02530
MIT1002_02531: MIT1002_02531 - Aspartate-semialdehyde dehydrogenase 2, at 2,825,647 to 2,826,663
_02531
Position (kb)
2820
2821
2822
2823
2824
2825
2826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 2819.276 kb on - strand at 2819.276 kb on - strand at 2819.296 kb on - strand, within MIT1002_02529 at 2819.327 kb on - strand, within MIT1002_02529 at 2819.387 kb on - strand, within MIT1002_02529 at 2819.539 kb on + strand, within MIT1002_02529 at 2819.554 kb on - strand, within MIT1002_02529 at 2819.572 kb on - strand, within MIT1002_02529 at 2819.614 kb on + strand, within MIT1002_02529 at 2819.732 kb on + strand, within MIT1002_02529 at 2819.737 kb on + strand, within MIT1002_02529 at 2819.745 kb on + strand, within MIT1002_02529 at 2819.745 kb on - strand, within MIT1002_02529 at 2819.745 kb on - strand, within MIT1002_02529 at 2819.781 kb on - strand, within MIT1002_02529 at 2819.797 kb on + strand, within MIT1002_02529 at 2819.837 kb on - strand, within MIT1002_02529 at 2819.841 kb on - strand, within MIT1002_02529 at 2819.882 kb on + strand, within MIT1002_02529 at 2819.959 kb on - strand at 2819.962 kb on - strand at 2819.962 kb on - strand at 2819.967 kb on - strand at 2819.981 kb on + strand at 2819.981 kb on + strand at 2820.006 kb on + strand at 2820.014 kb on - strand at 2820.014 kb on - strand at 2820.035 kb on + strand at 2820.043 kb on - strand at 2820.177 kb on + strand at 2820.252 kb on + strand at 2820.294 kb on - strand at 2820.295 kb on + strand at 2820.345 kb on - strand at 2820.352 kb on - strand at 2820.397 kb on - strand at 2820.404 kb on - strand at 2820.416 kb on - strand at 2820.453 kb on - strand at 2820.617 kb on + strand at 2820.617 kb on - strand at 2820.665 kb on + strand at 2820.730 kb on + strand, within MIT1002_02530 at 2820.795 kb on - strand, within MIT1002_02530 at 2820.950 kb on + strand, within MIT1002_02530 at 2821.119 kb on - strand, within MIT1002_02530 at 2821.157 kb on + strand, within MIT1002_02530 at 2821.157 kb on - strand, within MIT1002_02530 at 2821.186 kb on - strand, within MIT1002_02530 at 2821.261 kb on + strand, within MIT1002_02530 at 2821.261 kb on + strand, within MIT1002_02530 at 2821.269 kb on - strand, within MIT1002_02530 at 2821.315 kb on - strand, within MIT1002_02530 at 2821.315 kb on - strand, within MIT1002_02530 at 2821.317 kb on + strand, within MIT1002_02530 at 2821.317 kb on + strand, within MIT1002_02530 at 2821.317 kb on + strand, within MIT1002_02530 at 2821.349 kb on + strand, within MIT1002_02530 at 2821.415 kb on + strand, within MIT1002_02530 at 2821.415 kb on - strand, within MIT1002_02530 at 2821.538 kb on - strand, within MIT1002_02530 at 2821.538 kb on - strand, within MIT1002_02530 at 2821.554 kb on + strand, within MIT1002_02530 at 2821.575 kb on - strand, within MIT1002_02530 at 2821.596 kb on + strand, within MIT1002_02530 at 2821.614 kb on + strand, within MIT1002_02530 at 2821.614 kb on + strand, within MIT1002_02530 at 2821.700 kb on + strand, within MIT1002_02530 at 2821.730 kb on - strand, within MIT1002_02530 at 2822.446 kb on - strand, within MIT1002_02530 at 2822.446 kb on - strand, within MIT1002_02530 at 2822.446 kb on - strand, within MIT1002_02530 at 2822.446 kb on - strand, within MIT1002_02530 at 2822.472 kb on + strand, within MIT1002_02530 at 2822.558 kb on - strand, within MIT1002_02530 at 2822.582 kb on - strand, within MIT1002_02530 at 2822.594 kb on - strand, within MIT1002_02530 at 2822.642 kb on - strand, within MIT1002_02530 at 2822.885 kb on + strand, within MIT1002_02530 at 2822.886 kb on + strand, within MIT1002_02530 at 2822.941 kb on - strand, within MIT1002_02530 at 2822.966 kb on - strand, within MIT1002_02530 at 2823.165 kb on + strand, within MIT1002_02530 at 2823.239 kb on + strand, within MIT1002_02530 at 2823.455 kb on + strand, within MIT1002_02530 at 2823.475 kb on - strand, within MIT1002_02530 at 2823.531 kb on + strand, within MIT1002_02530 at 2823.660 kb on + strand, within MIT1002_02530 at 2823.668 kb on - strand, within MIT1002_02530 at 2823.688 kb on + strand, within MIT1002_02530 at 2823.706 kb on + strand, within MIT1002_02530 at 2823.867 kb on + strand, within MIT1002_02530 at 2823.877 kb on + strand, within MIT1002_02530 at 2823.877 kb on + strand, within MIT1002_02530 at 2823.939 kb on - strand, within MIT1002_02530 at 2824.013 kb on - strand, within MIT1002_02530 at 2824.049 kb on + strand, within MIT1002_02530 at 2824.057 kb on - strand, within MIT1002_02530 at 2824.121 kb on + strand, within MIT1002_02530 at 2824.127 kb on + strand, within MIT1002_02530 at 2824.174 kb on - strand, within MIT1002_02530 at 2824.176 kb on + strand, within MIT1002_02530 at 2824.289 kb on + strand, within MIT1002_02530 at 2824.340 kb on - strand, within MIT1002_02530 at 2824.350 kb on + strand, within MIT1002_02530 at 2824.410 kb on + strand, within MIT1002_02530 at 2824.418 kb on - strand, within MIT1002_02530 at 2824.475 kb on + strand, within MIT1002_02530 at 2824.476 kb on + strand, within MIT1002_02530 at 2824.493 kb on - strand, within MIT1002_02530 at 2824.493 kb on - strand, within MIT1002_02530 at 2824.669 kb on + strand at 2824.763 kb on + strand at 2824.763 kb on + strand at 2824.778 kb on + strand at 2824.781 kb on - strand at 2824.786 kb on - strand at 2824.879 kb on + strand at 2824.899 kb on - strand at 2825.023 kb on + strand at 2825.141 kb on - strand at 2825.152 kb on + strand at 2825.274 kb on + strand at 2825.285 kb on - strand at 2825.338 kb on - strand at 2825.373 kb on - strand at 2825.378 kb on - strand at 2825.387 kb on + strand at 2825.387 kb on + strand at 2825.395 kb on - strand at 2825.395 kb on - strand at 2825.431 kb on - strand at 2825.550 kb on + strand at 2825.550 kb on + strand at 2825.550 kb on + strand at 2825.551 kb on + strand at 2825.689 kb on + strand at 2825.724 kb on - strand at 2825.741 kb on + strand at 2825.741 kb on + strand at 2825.812 kb on - strand, within MIT1002_02531 at 2825.846 kb on + strand, within MIT1002_02531 at 2825.872 kb on - strand, within MIT1002_02531 at 2825.957 kb on + strand, within MIT1002_02531
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 2,819,276 - -0.2 2,819,276 - -1.2 2,819,296 - MIT1002_02529 0.11 -1.8 2,819,327 - MIT1002_02529 0.15 -0.5 2,819,387 - MIT1002_02529 0.22 -3.2 2,819,539 + MIT1002_02529 0.42 +8.0 2,819,554 - MIT1002_02529 0.43 -0.8 2,819,572 - MIT1002_02529 0.46 -0.8 2,819,614 + MIT1002_02529 0.51 -0.5 2,819,732 + MIT1002_02529 0.66 +0.9 2,819,737 + MIT1002_02529 0.67 -1.2 2,819,745 + MIT1002_02529 0.68 -1.1 2,819,745 - MIT1002_02529 0.68 -2.4 2,819,745 - MIT1002_02529 0.68 -1.3 2,819,781 - MIT1002_02529 0.72 -2.7 2,819,797 + MIT1002_02529 0.74 +1.7 2,819,837 - MIT1002_02529 0.79 -2.2 2,819,841 - MIT1002_02529 0.80 -0.9 2,819,882 + MIT1002_02529 0.85 -0.2 2,819,959 - -2.4 2,819,962 - -2.8 2,819,962 - -1.8 2,819,967 - +0.1 2,819,981 + -0.9 2,819,981 + -1.4 2,820,006 + -0.8 2,820,014 - -0.6 2,820,014 - -0.3 2,820,035 + -0.2 2,820,043 - -1.4 2,820,177 + +0.6 2,820,252 + +0.7 2,820,294 - +0.7 2,820,295 + -2.6 2,820,345 - +0.0 2,820,352 - -0.3 2,820,397 - -0.1 2,820,404 - +0.5 2,820,416 - +0.0 2,820,453 - -0.5 2,820,617 + -2.2 2,820,617 - -2.2 2,820,665 + -0.3 2,820,730 + MIT1002_02530 0.11 -1.9 2,820,795 - MIT1002_02530 0.12 -1.0 2,820,950 + MIT1002_02530 0.15 +0.6 2,821,119 - MIT1002_02530 0.19 +0.3 2,821,157 + MIT1002_02530 0.19 -0.6 2,821,157 - MIT1002_02530 0.19 -1.1 2,821,186 - MIT1002_02530 0.20 -1.0 2,821,261 + MIT1002_02530 0.21 +1.0 2,821,261 + MIT1002_02530 0.21 +0.3 2,821,269 - MIT1002_02530 0.22 -0.4 2,821,315 - MIT1002_02530 0.23 -1.1 2,821,315 - MIT1002_02530 0.23 -0.3 2,821,317 + MIT1002_02530 0.23 -0.5 2,821,317 + MIT1002_02530 0.23 -0.8 2,821,317 + MIT1002_02530 0.23 +1.4 2,821,349 + MIT1002_02530 0.23 -1.0 2,821,415 + MIT1002_02530 0.25 +0.9 2,821,415 - MIT1002_02530 0.25 +0.3 2,821,538 - MIT1002_02530 0.27 -1.6 2,821,538 - MIT1002_02530 0.27 -0.5 2,821,554 + MIT1002_02530 0.27 -2.4 2,821,575 - MIT1002_02530 0.28 -0.2 2,821,596 + MIT1002_02530 0.28 +0.4 2,821,614 + MIT1002_02530 0.29 -0.1 2,821,614 + MIT1002_02530 0.29 -0.1 2,821,700 + MIT1002_02530 0.30 -0.2 2,821,730 - MIT1002_02530 0.31 -0.2 2,822,446 - MIT1002_02530 0.46 -1.0 2,822,446 - MIT1002_02530 0.46 -1.7 2,822,446 - MIT1002_02530 0.46 +0.1 2,822,446 - MIT1002_02530 0.46 +0.5 2,822,472 + MIT1002_02530 0.46 -1.0 2,822,558 - MIT1002_02530 0.48 -0.3 2,822,582 - MIT1002_02530 0.48 +0.6 2,822,594 - MIT1002_02530 0.49 -0.7 2,822,642 - MIT1002_02530 0.49 -0.5 2,822,885 + MIT1002_02530 0.54 -1.5 2,822,886 + MIT1002_02530 0.54 -0.9 2,822,941 - MIT1002_02530 0.56 +0.1 2,822,966 - MIT1002_02530 0.56 +0.5 2,823,165 + MIT1002_02530 0.60 -1.4 2,823,239 + MIT1002_02530 0.62 -2.8 2,823,455 + MIT1002_02530 0.66 -0.6 2,823,475 - MIT1002_02530 0.66 -2.1 2,823,531 + MIT1002_02530 0.68 +0.4 2,823,660 + MIT1002_02530 0.70 -0.8 2,823,668 - MIT1002_02530 0.70 -0.9 2,823,688 + MIT1002_02530 0.71 +1.6 2,823,706 + MIT1002_02530 0.71 -1.2 2,823,867 + MIT1002_02530 0.74 +0.5 2,823,877 + MIT1002_02530 0.75 -1.5 2,823,877 + MIT1002_02530 0.75 -2.5 2,823,939 - MIT1002_02530 0.76 -0.9 2,824,013 - MIT1002_02530 0.77 -0.3 2,824,049 + MIT1002_02530 0.78 -0.4 2,824,057 - MIT1002_02530 0.78 -0.1 2,824,121 + MIT1002_02530 0.80 -2.6 2,824,127 + MIT1002_02530 0.80 -1.9 2,824,174 - MIT1002_02530 0.81 -0.4 2,824,176 + MIT1002_02530 0.81 -1.1 2,824,289 + MIT1002_02530 0.83 -0.3 2,824,340 - MIT1002_02530 0.84 -1.8 2,824,350 + MIT1002_02530 0.84 -0.9 2,824,410 + MIT1002_02530 0.85 -0.8 2,824,418 - MIT1002_02530 0.86 +0.5 2,824,475 + MIT1002_02530 0.87 -0.8 2,824,476 + MIT1002_02530 0.87 -3.1 2,824,493 - MIT1002_02530 0.87 -0.8 2,824,493 - MIT1002_02530 0.87 -0.2 2,824,669 + +0.2 2,824,763 + +0.1 2,824,763 + +0.2 2,824,778 + -0.6 2,824,781 - -0.5 2,824,786 - -0.9 2,824,879 + -0.3 2,824,899 - -0.8 2,825,023 + -1.5 2,825,141 - -2.2 2,825,152 + -0.8 2,825,274 + -0.5 2,825,285 - +0.7 2,825,338 - -0.1 2,825,373 - -0.2 2,825,378 - +2.7 2,825,387 + +0.8 2,825,387 + +0.8 2,825,395 - +0.3 2,825,395 - -0.3 2,825,431 - +1.3 2,825,550 + +1.0 2,825,550 + -0.8 2,825,550 + +0.6 2,825,551 + +0.6 2,825,689 + +1.1 2,825,724 - -0.5 2,825,741 + -0.1 2,825,741 + -0.1 2,825,812 - MIT1002_02531 0.16 -1.2 2,825,846 + MIT1002_02531 0.20 -0.6 2,825,872 - MIT1002_02531 0.22 -1.0 2,825,957 + MIT1002_02531 0.30 -1.7
Or see this region's nucleotide sequence