Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02259 and MIT1002_02260 are separated by 14 nucleotides MIT1002_02260 and MIT1002_02261 are separated by 114 nucleotides MIT1002_02261 and MIT1002_02262 are separated by 205 nucleotides
MIT1002_02259: MIT1002_02259 - Gamma-glutamyltranspeptidase precursor, at 2,512,864 to 2,514,621
_02259
MIT1002_02260: MIT1002_02260 - 2,3-dehydroadipyl-CoA hydratase, at 2,514,636 to 2,515,400
_02260
MIT1002_02261: MIT1002_02261 - rhombosortase, at 2,515,515 to 2,516,096
_02261
MIT1002_02262: MIT1002_02262 - Prolyl tripeptidyl peptidase precursor, at 2,516,302 to 2,518,797
_02262
Position (kb)
2515
2516
2517 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2514.566 kb on + strand at 2514.576 kb on + strand at 2514.584 kb on - strand at 2514.772 kb on + strand, within MIT1002_02260 at 2514.772 kb on + strand, within MIT1002_02260 at 2514.780 kb on - strand, within MIT1002_02260 at 2514.805 kb on + strand, within MIT1002_02260 at 2514.831 kb on - strand, within MIT1002_02260 at 2514.912 kb on + strand, within MIT1002_02260 at 2514.912 kb on + strand, within MIT1002_02260 at 2514.912 kb on + strand, within MIT1002_02260 at 2514.912 kb on + strand, within MIT1002_02260 at 2514.918 kb on - strand, within MIT1002_02260 at 2514.920 kb on - strand, within MIT1002_02260 at 2514.920 kb on - strand, within MIT1002_02260 at 2514.920 kb on - strand, within MIT1002_02260 at 2514.920 kb on - strand, within MIT1002_02260 at 2514.920 kb on - strand, within MIT1002_02260 at 2514.920 kb on - strand, within MIT1002_02260 at 2514.929 kb on + strand, within MIT1002_02260 at 2514.929 kb on + strand, within MIT1002_02260 at 2514.929 kb on + strand, within MIT1002_02260 at 2514.929 kb on + strand, within MIT1002_02260 at 2514.937 kb on - strand, within MIT1002_02260 at 2514.937 kb on - strand, within MIT1002_02260 at 2514.937 kb on - strand, within MIT1002_02260 at 2514.957 kb on + strand, within MIT1002_02260 at 2515.094 kb on + strand, within MIT1002_02260 at 2515.110 kb on - strand, within MIT1002_02260 at 2515.157 kb on - strand, within MIT1002_02260 at 2515.347 kb on + strand at 2515.352 kb on + strand at 2515.354 kb on - strand at 2515.359 kb on + strand at 2515.360 kb on - strand at 2515.360 kb on - strand at 2515.373 kb on + strand at 2515.509 kb on + strand at 2515.519 kb on - strand at 2515.524 kb on - strand at 2515.538 kb on + strand at 2515.538 kb on + strand at 2515.538 kb on + strand at 2515.727 kb on + strand, within MIT1002_02261 at 2515.727 kb on + strand, within MIT1002_02261 at 2515.734 kb on + strand, within MIT1002_02261 at 2515.735 kb on - strand, within MIT1002_02261 at 2515.735 kb on - strand, within MIT1002_02261 at 2515.787 kb on + strand, within MIT1002_02261 at 2515.848 kb on + strand, within MIT1002_02261 at 2515.856 kb on - strand, within MIT1002_02261 at 2515.856 kb on - strand, within MIT1002_02261 at 2515.938 kb on + strand, within MIT1002_02261 at 2515.946 kb on - strand, within MIT1002_02261 at 2515.960 kb on - strand, within MIT1002_02261 at 2516.042 kb on + strand at 2516.250 kb on + strand at 2516.253 kb on + strand at 2516.261 kb on - strand at 2516.346 kb on - strand at 2516.354 kb on + strand at 2516.364 kb on + strand at 2516.372 kb on - strand at 2516.631 kb on + strand, within MIT1002_02262 at 2516.639 kb on - strand, within MIT1002_02262 at 2516.720 kb on + strand, within MIT1002_02262 at 2516.781 kb on + strand, within MIT1002_02262 at 2516.789 kb on - strand, within MIT1002_02262 at 2516.868 kb on - strand, within MIT1002_02262 at 2516.919 kb on - strand, within MIT1002_02262 at 2516.957 kb on - strand, within MIT1002_02262 at 2516.957 kb on - strand, within MIT1002_02262 at 2516.957 kb on - strand, within MIT1002_02262 at 2517.073 kb on - strand, within MIT1002_02262
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 2,514,566 + -0.5 2,514,576 + -0.7 2,514,584 - -1.2 2,514,772 + MIT1002_02260 0.18 +0.3 2,514,772 + MIT1002_02260 0.18 -1.3 2,514,780 - MIT1002_02260 0.19 -1.2 2,514,805 + MIT1002_02260 0.22 +1.0 2,514,831 - MIT1002_02260 0.25 +0.6 2,514,912 + MIT1002_02260 0.36 -0.2 2,514,912 + MIT1002_02260 0.36 -0.1 2,514,912 + MIT1002_02260 0.36 +0.9 2,514,912 + MIT1002_02260 0.36 +0.0 2,514,918 - MIT1002_02260 0.37 +0.6 2,514,920 - MIT1002_02260 0.37 +0.3 2,514,920 - MIT1002_02260 0.37 +1.0 2,514,920 - MIT1002_02260 0.37 -1.3 2,514,920 - MIT1002_02260 0.37 -0.5 2,514,920 - MIT1002_02260 0.37 +1.1 2,514,920 - MIT1002_02260 0.37 +0.6 2,514,929 + MIT1002_02260 0.38 +0.2 2,514,929 + MIT1002_02260 0.38 -0.7 2,514,929 + MIT1002_02260 0.38 -0.4 2,514,929 + MIT1002_02260 0.38 +1.5 2,514,937 - MIT1002_02260 0.39 -0.5 2,514,937 - MIT1002_02260 0.39 +0.5 2,514,937 - MIT1002_02260 0.39 +0.1 2,514,957 + MIT1002_02260 0.42 -0.5 2,515,094 + MIT1002_02260 0.60 -1.1 2,515,110 - MIT1002_02260 0.62 +0.5 2,515,157 - MIT1002_02260 0.68 +0.7 2,515,347 + +0.5 2,515,352 + +0.0 2,515,354 - -1.3 2,515,359 + -0.1 2,515,360 - +0.6 2,515,360 - -0.1 2,515,373 + +1.0 2,515,509 + -1.1 2,515,519 - -1.7 2,515,524 - -2.2 2,515,538 + -0.9 2,515,538 + -1.8 2,515,538 + -0.1 2,515,727 + MIT1002_02261 0.36 -2.6 2,515,727 + MIT1002_02261 0.36 -0.5 2,515,734 + MIT1002_02261 0.38 -2.6 2,515,735 - MIT1002_02261 0.38 -2.6 2,515,735 - MIT1002_02261 0.38 -1.8 2,515,787 + MIT1002_02261 0.47 -1.8 2,515,848 + MIT1002_02261 0.57 -1.3 2,515,856 - MIT1002_02261 0.59 -1.9 2,515,856 - MIT1002_02261 0.59 -2.3 2,515,938 + MIT1002_02261 0.73 -1.8 2,515,946 - MIT1002_02261 0.74 -3.6 2,515,960 - MIT1002_02261 0.76 +0.3 2,516,042 + -1.3 2,516,250 + -1.7 2,516,253 + -0.2 2,516,261 - -0.3 2,516,346 - -1.3 2,516,354 + -1.4 2,516,364 + -1.2 2,516,372 - -0.3 2,516,631 + MIT1002_02262 0.13 +1.2 2,516,639 - MIT1002_02262 0.14 -0.7 2,516,720 + MIT1002_02262 0.17 -1.0 2,516,781 + MIT1002_02262 0.19 -0.6 2,516,789 - MIT1002_02262 0.20 -0.1 2,516,868 - MIT1002_02262 0.23 -0.2 2,516,919 - MIT1002_02262 0.25 -0.8 2,516,957 - MIT1002_02262 0.26 -1.1 2,516,957 - MIT1002_02262 0.26 +0.8 2,516,957 - MIT1002_02262 0.26 -0.5 2,517,073 - MIT1002_02262 0.31 +0.6
Or see this region's nucleotide sequence