Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02103 and MIT1002_02104 overlap by 4 nucleotides MIT1002_02104 and MIT1002_02105 overlap by 4 nucleotides MIT1002_02105 and MIT1002_02106 are separated by 353 nucleotides MIT1002_02106 and MIT1002_02107 overlap by 22 nucleotides
MIT1002_02103: MIT1002_02103 - Transposase, at 2,363,655 to 2,363,948
_02103
MIT1002_02104: MIT1002_02104 - putative transposase OrfB, at 2,363,945 to 2,364,823
_02104
MIT1002_02105: MIT1002_02105 - Tetrachloro-P-hydroquinone reductive dehalogenase, at 2,364,820 to 2,365,035
_02105
MIT1002_02106: MIT1002_02106 - hypothetical protein, at 2,365,389 to 2,365,892
_02106
MIT1002_02107: MIT1002_02107 - thiamine biosynthesis protein ThiF, at 2,365,871 to 2,367,625
_02107
Position (kb)
2364
2365
2366 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2363.836 kb on - strand, within MIT1002_02103 at 2363.847 kb on - strand, within MIT1002_02103 at 2363.977 kb on + strand at 2364.029 kb on - strand at 2364.058 kb on + strand, within MIT1002_02104 at 2364.103 kb on + strand, within MIT1002_02104 at 2364.106 kb on - strand, within MIT1002_02104 at 2364.106 kb on - strand, within MIT1002_02104 at 2364.106 kb on - strand, within MIT1002_02104 at 2364.106 kb on - strand, within MIT1002_02104 at 2364.122 kb on + strand, within MIT1002_02104 at 2364.131 kb on - strand, within MIT1002_02104 at 2364.177 kb on - strand, within MIT1002_02104 at 2364.198 kb on - strand, within MIT1002_02104 at 2364.243 kb on - strand, within MIT1002_02104 at 2364.251 kb on + strand, within MIT1002_02104 at 2364.281 kb on + strand, within MIT1002_02104 at 2364.289 kb on - strand, within MIT1002_02104 at 2364.306 kb on + strand, within MIT1002_02104 at 2364.306 kb on + strand, within MIT1002_02104 at 2364.314 kb on - strand, within MIT1002_02104 at 2364.314 kb on - strand, within MIT1002_02104 at 2364.321 kb on + strand, within MIT1002_02104 at 2364.358 kb on - strand, within MIT1002_02104 at 2364.371 kb on + strand, within MIT1002_02104 at 2364.371 kb on + strand, within MIT1002_02104 at 2364.435 kb on - strand, within MIT1002_02104 at 2364.462 kb on - strand, within MIT1002_02104 at 2364.479 kb on + strand, within MIT1002_02104 at 2364.501 kb on + strand, within MIT1002_02104 at 2364.502 kb on + strand, within MIT1002_02104 at 2364.577 kb on - strand, within MIT1002_02104 at 2364.582 kb on - strand, within MIT1002_02104 at 2364.582 kb on - strand, within MIT1002_02104 at 2364.582 kb on - strand, within MIT1002_02104 at 2364.637 kb on - strand, within MIT1002_02104 at 2364.656 kb on - strand, within MIT1002_02104 at 2364.665 kb on + strand, within MIT1002_02104 at 2364.675 kb on + strand, within MIT1002_02104 at 2364.703 kb on + strand, within MIT1002_02104 at 2364.754 kb on + strand at 2364.821 kb on + strand at 2364.857 kb on - strand, within MIT1002_02105 at 2364.858 kb on - strand, within MIT1002_02105 at 2364.862 kb on - strand, within MIT1002_02105 at 2364.864 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.001 kb on + strand, within MIT1002_02105 at 2365.009 kb on - strand, within MIT1002_02105 at 2365.009 kb on - strand, within MIT1002_02105 at 2365.009 kb on - strand, within MIT1002_02105 at 2365.011 kb on - strand, within MIT1002_02105 at 2365.019 kb on + strand at 2365.022 kb on - strand at 2365.022 kb on - strand at 2365.024 kb on + strand at 2365.027 kb on - strand at 2365.027 kb on - strand at 2365.027 kb on - strand at 2365.034 kb on - strand at 2365.041 kb on + strand at 2365.049 kb on + strand at 2365.057 kb on - strand at 2365.170 kb on + strand at 2365.259 kb on + strand at 2365.259 kb on + strand at 2365.259 kb on + strand at 2365.267 kb on - strand at 2365.267 kb on - strand at 2365.372 kb on + strand at 2365.372 kb on + strand at 2365.380 kb on - strand at 2365.394 kb on - strand at 2365.394 kb on - strand at 2365.458 kb on - strand, within MIT1002_02106 at 2365.522 kb on + strand, within MIT1002_02106 at 2365.523 kb on + strand, within MIT1002_02106 at 2365.523 kb on + strand, within MIT1002_02106 at 2365.523 kb on + strand, within MIT1002_02106 at 2365.523 kb on + strand, within MIT1002_02106 at 2365.531 kb on - strand, within MIT1002_02106 at 2365.578 kb on - strand, within MIT1002_02106 at 2365.583 kb on - strand, within MIT1002_02106 at 2365.628 kb on + strand, within MIT1002_02106 at 2365.683 kb on - strand, within MIT1002_02106 at 2365.685 kb on + strand, within MIT1002_02106 at 2365.693 kb on - strand, within MIT1002_02106 at 2365.697 kb on + strand, within MIT1002_02106 at 2365.749 kb on - strand, within MIT1002_02106 at 2365.758 kb on + strand, within MIT1002_02106 at 2365.763 kb on + strand, within MIT1002_02106 at 2365.868 kb on + strand at 2365.869 kb on + strand at 2365.876 kb on - strand at 2365.909 kb on + strand at 2365.944 kb on + strand at 2365.944 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 2,363,836 - MIT1002_02103 0.62 -0.2 2,363,847 - MIT1002_02103 0.65 -0.3 2,363,977 + -0.2 2,364,029 - +0.6 2,364,058 + MIT1002_02104 0.13 -1.1 2,364,103 + MIT1002_02104 0.18 +0.7 2,364,106 - MIT1002_02104 0.18 -1.0 2,364,106 - MIT1002_02104 0.18 +0.5 2,364,106 - MIT1002_02104 0.18 +0.0 2,364,106 - MIT1002_02104 0.18 -0.1 2,364,122 + MIT1002_02104 0.20 -0.0 2,364,131 - MIT1002_02104 0.21 +0.9 2,364,177 - MIT1002_02104 0.26 -1.0 2,364,198 - MIT1002_02104 0.29 -1.5 2,364,243 - MIT1002_02104 0.34 -0.2 2,364,251 + MIT1002_02104 0.35 +0.3 2,364,281 + MIT1002_02104 0.38 -0.1 2,364,289 - MIT1002_02104 0.39 +1.0 2,364,306 + MIT1002_02104 0.41 -3.4 2,364,306 + MIT1002_02104 0.41 -0.2 2,364,314 - MIT1002_02104 0.42 -0.5 2,364,314 - MIT1002_02104 0.42 -0.3 2,364,321 + MIT1002_02104 0.43 -0.9 2,364,358 - MIT1002_02104 0.47 +2.0 2,364,371 + MIT1002_02104 0.48 +0.3 2,364,371 + MIT1002_02104 0.48 -0.7 2,364,435 - MIT1002_02104 0.56 +0.0 2,364,462 - MIT1002_02104 0.59 +0.2 2,364,479 + MIT1002_02104 0.61 +1.8 2,364,501 + MIT1002_02104 0.63 +0.6 2,364,502 + MIT1002_02104 0.63 +0.0 2,364,577 - MIT1002_02104 0.72 +1.7 2,364,582 - MIT1002_02104 0.72 -0.4 2,364,582 - MIT1002_02104 0.72 +0.5 2,364,582 - MIT1002_02104 0.72 +0.2 2,364,637 - MIT1002_02104 0.79 +0.1 2,364,656 - MIT1002_02104 0.81 -0.2 2,364,665 + MIT1002_02104 0.82 -0.3 2,364,675 + MIT1002_02104 0.83 +3.5 2,364,703 + MIT1002_02104 0.86 +2.2 2,364,754 + +0.0 2,364,821 + -1.3 2,364,857 - MIT1002_02105 0.17 +1.2 2,364,858 - MIT1002_02105 0.18 -0.1 2,364,862 - MIT1002_02105 0.19 +0.1 2,364,864 + MIT1002_02105 0.20 -2.5 2,365,001 + MIT1002_02105 0.84 -1.0 2,365,001 + MIT1002_02105 0.84 -0.2 2,365,001 + MIT1002_02105 0.84 +3.5 2,365,001 + MIT1002_02105 0.84 -0.1 2,365,001 + MIT1002_02105 0.84 +1.9 2,365,001 + MIT1002_02105 0.84 -2.4 2,365,001 + MIT1002_02105 0.84 +0.0 2,365,001 + MIT1002_02105 0.84 +0.0 2,365,009 - MIT1002_02105 0.88 +0.4 2,365,009 - MIT1002_02105 0.88 +0.9 2,365,009 - MIT1002_02105 0.88 -0.6 2,365,011 - MIT1002_02105 0.88 -0.9 2,365,019 + -0.8 2,365,022 - -1.4 2,365,022 - -1.5 2,365,024 + -0.1 2,365,027 - +0.3 2,365,027 - +0.8 2,365,027 - +0.3 2,365,034 - -0.7 2,365,041 + -0.3 2,365,049 + +0.8 2,365,057 - +0.2 2,365,170 + -0.2 2,365,259 + +0.1 2,365,259 + -0.5 2,365,259 + -1.8 2,365,267 - -0.0 2,365,267 - -0.1 2,365,372 + +0.6 2,365,372 + -0.8 2,365,380 - -0.6 2,365,394 - -0.4 2,365,394 - +0.0 2,365,458 - MIT1002_02106 0.14 -1.8 2,365,522 + MIT1002_02106 0.26 +0.7 2,365,523 + MIT1002_02106 0.27 +0.0 2,365,523 + MIT1002_02106 0.27 +0.9 2,365,523 + MIT1002_02106 0.27 +0.1 2,365,523 + MIT1002_02106 0.27 -0.9 2,365,531 - MIT1002_02106 0.28 -0.2 2,365,578 - MIT1002_02106 0.38 +0.2 2,365,583 - MIT1002_02106 0.38 +1.3 2,365,628 + MIT1002_02106 0.47 +1.2 2,365,683 - MIT1002_02106 0.58 +2.0 2,365,685 + MIT1002_02106 0.59 +0.2 2,365,693 - MIT1002_02106 0.60 +0.4 2,365,697 + MIT1002_02106 0.61 +0.9 2,365,749 - MIT1002_02106 0.71 -0.1 2,365,758 + MIT1002_02106 0.73 +0.4 2,365,763 + MIT1002_02106 0.74 +0.2 2,365,868 + +0.6 2,365,869 + -0.5 2,365,876 - +0.0 2,365,909 + +0.2 2,365,944 + -2.2 2,365,944 + +0.3
Or see this region's nucleotide sequence