Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01408 and MIT1002_01409 are separated by 59 nucleotides MIT1002_01409 and MIT1002_01410 overlap by 4 nucleotides MIT1002_01410 and MIT1002_01411 are separated by 293 nucleotides MIT1002_01411 and MIT1002_01412 are separated by 29 nucleotides
MIT1002_01408: MIT1002_01408 - Zinc carboxypeptidase, at 1,553,723 to 1,554,628
_01408
MIT1002_01409: MIT1002_01409 - hypothetical protein, at 1,554,688 to 1,554,909
_01409
MIT1002_01410: MIT1002_01410 - putative Fe-S protein, at 1,554,906 to 1,555,094
_01410
MIT1002_01411: MIT1002_01411 - putative tRNA-dihydrouridine synthase, at 1,555,388 to 1,556,326
_01411
MIT1002_01412: MIT1002_01412 - Transposase, at 1,556,356 to 1,557,330
_01412
Position (kb)
1555
1556
1557 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1554.433 kb on + strand, within MIT1002_01408 at 1554.441 kb on + strand, within MIT1002_01408 at 1554.441 kb on + strand, within MIT1002_01408 at 1554.441 kb on - strand, within MIT1002_01408 at 1554.454 kb on - strand, within MIT1002_01408 at 1554.510 kb on - strand, within MIT1002_01408 at 1554.511 kb on - strand, within MIT1002_01408 at 1554.531 kb on + strand, within MIT1002_01408 at 1554.531 kb on + strand, within MIT1002_01408 at 1554.538 kb on + strand at 1554.538 kb on + strand at 1554.571 kb on - strand at 1554.590 kb on - strand at 1554.590 kb on - strand at 1554.739 kb on + strand, within MIT1002_01409 at 1554.739 kb on + strand, within MIT1002_01409 at 1554.741 kb on + strand, within MIT1002_01409 at 1554.747 kb on - strand, within MIT1002_01409 at 1554.748 kb on - strand, within MIT1002_01409 at 1554.749 kb on - strand, within MIT1002_01409 at 1554.761 kb on - strand, within MIT1002_01409 at 1554.888 kb on + strand at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.925 kb on + strand, within MIT1002_01410 at 1554.933 kb on - strand, within MIT1002_01410 at 1554.933 kb on - strand, within MIT1002_01410 at 1554.933 kb on - strand, within MIT1002_01410 at 1554.933 kb on - strand, within MIT1002_01410 at 1554.933 kb on - strand, within MIT1002_01410 at 1554.933 kb on - strand, within MIT1002_01410 at 1554.956 kb on + strand, within MIT1002_01410 at 1554.968 kb on + strand, within MIT1002_01410 at 1555.075 kb on + strand, within MIT1002_01410 at 1555.084 kb on - strand at 1555.084 kb on - strand at 1555.092 kb on - strand at 1555.102 kb on - strand at 1555.164 kb on + strand at 1555.172 kb on + strand at 1555.276 kb on + strand at 1555.292 kb on - strand at 1555.404 kb on + strand at 1555.429 kb on - strand at 1555.450 kb on - strand at 1555.469 kb on - strand at 1555.536 kb on + strand, within MIT1002_01411 at 1555.579 kb on - strand, within MIT1002_01411 at 1555.593 kb on + strand, within MIT1002_01411 at 1555.612 kb on - strand, within MIT1002_01411 at 1555.612 kb on - strand, within MIT1002_01411 at 1555.651 kb on - strand, within MIT1002_01411 at 1555.667 kb on - strand, within MIT1002_01411 at 1555.694 kb on - strand, within MIT1002_01411 at 1555.702 kb on + strand, within MIT1002_01411 at 1555.742 kb on + strand, within MIT1002_01411 at 1555.742 kb on - strand, within MIT1002_01411 at 1555.760 kb on + strand, within MIT1002_01411 at 1555.823 kb on - strand, within MIT1002_01411 at 1555.872 kb on + strand, within MIT1002_01411 at 1555.872 kb on + strand, within MIT1002_01411 at 1555.920 kb on - strand, within MIT1002_01411 at 1555.933 kb on + strand, within MIT1002_01411 at 1555.946 kb on - strand, within MIT1002_01411 at 1556.091 kb on + strand, within MIT1002_01411 at 1556.091 kb on + strand, within MIT1002_01411 at 1556.091 kb on + strand, within MIT1002_01411 at 1556.091 kb on + strand, within MIT1002_01411 at 1556.091 kb on + strand, within MIT1002_01411 at 1556.099 kb on - strand, within MIT1002_01411 at 1556.111 kb on - strand, within MIT1002_01411 at 1556.121 kb on + strand, within MIT1002_01411 at 1556.129 kb on - strand, within MIT1002_01411 at 1556.129 kb on - strand, within MIT1002_01411 at 1556.192 kb on - strand, within MIT1002_01411 at 1556.257 kb on - strand at 1556.294 kb on + strand at 1556.345 kb on + strand at 1556.362 kb on - strand at 1556.376 kb on - strand at 1556.385 kb on + strand at 1556.394 kb on + strand at 1556.453 kb on - strand at 1556.539 kb on + strand, within MIT1002_01412 at 1556.539 kb on + strand, within MIT1002_01412 at 1556.559 kb on + strand, within MIT1002_01412 at 1556.567 kb on - strand, within MIT1002_01412 at 1556.577 kb on - strand, within MIT1002_01412 at 1556.633 kb on + strand, within MIT1002_01412 at 1556.644 kb on - strand, within MIT1002_01412 at 1556.734 kb on + strand, within MIT1002_01412 at 1556.777 kb on + strand, within MIT1002_01412 at 1556.784 kb on + strand, within MIT1002_01412 at 1556.784 kb on + strand, within MIT1002_01412 at 1556.792 kb on - strand, within MIT1002_01412 at 1556.792 kb on - strand, within MIT1002_01412 at 1556.794 kb on + strand, within MIT1002_01412 at 1556.990 kb on + strand, within MIT1002_01412 at 1557.015 kb on + strand, within MIT1002_01412 at 1557.084 kb on - strand, within MIT1002_01412 at 1557.084 kb on - strand, within MIT1002_01412 at 1557.108 kb on - strand, within MIT1002_01412 at 1557.124 kb on + strand, within MIT1002_01412 at 1557.132 kb on - strand, within MIT1002_01412 at 1557.132 kb on - strand, within MIT1002_01412 at 1557.132 kb on - strand, within MIT1002_01412 at 1557.170 kb on + strand, within MIT1002_01412 at 1557.214 kb on + strand, within MIT1002_01412 at 1557.246 kb on + strand at 1557.287 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 1,554,433 + MIT1002_01408 0.78 -1.6 1,554,441 + MIT1002_01408 0.79 -0.4 1,554,441 + MIT1002_01408 0.79 +0.1 1,554,441 - MIT1002_01408 0.79 -1.0 1,554,454 - MIT1002_01408 0.81 +0.9 1,554,510 - MIT1002_01408 0.87 -1.4 1,554,511 - MIT1002_01408 0.87 -1.2 1,554,531 + MIT1002_01408 0.89 -0.5 1,554,531 + MIT1002_01408 0.89 -0.1 1,554,538 + +0.7 1,554,538 + -0.6 1,554,571 - -1.2 1,554,590 - +0.1 1,554,590 - -0.4 1,554,739 + MIT1002_01409 0.23 +0.5 1,554,739 + MIT1002_01409 0.23 -0.0 1,554,741 + MIT1002_01409 0.24 +0.6 1,554,747 - MIT1002_01409 0.27 +0.3 1,554,748 - MIT1002_01409 0.27 +1.5 1,554,749 - MIT1002_01409 0.27 -0.1 1,554,761 - MIT1002_01409 0.33 -2.4 1,554,888 + +1.0 1,554,925 + MIT1002_01410 0.10 +1.7 1,554,925 + MIT1002_01410 0.10 -0.1 1,554,925 + MIT1002_01410 0.10 +0.6 1,554,925 + MIT1002_01410 0.10 +1.1 1,554,925 + MIT1002_01410 0.10 +2.8 1,554,925 + MIT1002_01410 0.10 +1.0 1,554,925 + MIT1002_01410 0.10 -0.4 1,554,925 + MIT1002_01410 0.10 +0.5 1,554,933 - MIT1002_01410 0.14 -0.9 1,554,933 - MIT1002_01410 0.14 -0.2 1,554,933 - MIT1002_01410 0.14 -0.3 1,554,933 - MIT1002_01410 0.14 -0.2 1,554,933 - MIT1002_01410 0.14 -1.2 1,554,933 - MIT1002_01410 0.14 +2.3 1,554,956 + MIT1002_01410 0.26 -0.6 1,554,968 + MIT1002_01410 0.33 -0.4 1,555,075 + MIT1002_01410 0.89 +0.1 1,555,084 - -0.5 1,555,084 - +1.4 1,555,092 - +0.2 1,555,102 - -0.3 1,555,164 + +0.7 1,555,172 + +1.8 1,555,276 + -1.2 1,555,292 - +0.8 1,555,404 + +0.5 1,555,429 - -1.8 1,555,450 - +0.1 1,555,469 - +0.2 1,555,536 + MIT1002_01411 0.16 -0.7 1,555,579 - MIT1002_01411 0.20 +0.6 1,555,593 + MIT1002_01411 0.22 -1.4 1,555,612 - MIT1002_01411 0.24 -0.9 1,555,612 - MIT1002_01411 0.24 -1.9 1,555,651 - MIT1002_01411 0.28 +0.6 1,555,667 - MIT1002_01411 0.30 -2.4 1,555,694 - MIT1002_01411 0.33 -0.7 1,555,702 + MIT1002_01411 0.33 -1.4 1,555,742 + MIT1002_01411 0.38 +0.2 1,555,742 - MIT1002_01411 0.38 +1.0 1,555,760 + MIT1002_01411 0.40 +0.5 1,555,823 - MIT1002_01411 0.46 -0.0 1,555,872 + MIT1002_01411 0.52 -0.4 1,555,872 + MIT1002_01411 0.52 +0.8 1,555,920 - MIT1002_01411 0.57 -2.0 1,555,933 + MIT1002_01411 0.58 +0.3 1,555,946 - MIT1002_01411 0.59 -1.6 1,556,091 + MIT1002_01411 0.75 +2.2 1,556,091 + MIT1002_01411 0.75 -0.5 1,556,091 + MIT1002_01411 0.75 +0.6 1,556,091 + MIT1002_01411 0.75 +0.1 1,556,091 + MIT1002_01411 0.75 +0.1 1,556,099 - MIT1002_01411 0.76 -0.3 1,556,111 - MIT1002_01411 0.77 -0.2 1,556,121 + MIT1002_01411 0.78 +1.1 1,556,129 - MIT1002_01411 0.79 +0.2 1,556,129 - MIT1002_01411 0.79 -0.7 1,556,192 - MIT1002_01411 0.86 -1.7 1,556,257 - +0.6 1,556,294 + -0.6 1,556,345 + +0.2 1,556,362 - +0.4 1,556,376 - +0.5 1,556,385 + +0.2 1,556,394 + +0.3 1,556,453 - -1.5 1,556,539 + MIT1002_01412 0.19 +0.3 1,556,539 + MIT1002_01412 0.19 +0.1 1,556,559 + MIT1002_01412 0.21 -0.0 1,556,567 - MIT1002_01412 0.22 -0.3 1,556,577 - MIT1002_01412 0.23 +0.6 1,556,633 + MIT1002_01412 0.28 +0.1 1,556,644 - MIT1002_01412 0.30 +0.5 1,556,734 + MIT1002_01412 0.39 -0.3 1,556,777 + MIT1002_01412 0.43 -1.0 1,556,784 + MIT1002_01412 0.44 -0.0 1,556,784 + MIT1002_01412 0.44 -0.9 1,556,792 - MIT1002_01412 0.45 +1.6 1,556,792 - MIT1002_01412 0.45 +0.6 1,556,794 + MIT1002_01412 0.45 -1.0 1,556,990 + MIT1002_01412 0.65 -0.3 1,557,015 + MIT1002_01412 0.68 -1.4 1,557,084 - MIT1002_01412 0.75 +0.7 1,557,084 - MIT1002_01412 0.75 +0.1 1,557,108 - MIT1002_01412 0.77 +2.2 1,557,124 + MIT1002_01412 0.79 +0.6 1,557,132 - MIT1002_01412 0.80 -1.6 1,557,132 - MIT1002_01412 0.80 -1.2 1,557,132 - MIT1002_01412 0.80 +1.5 1,557,170 + MIT1002_01412 0.83 -0.7 1,557,214 + MIT1002_01412 0.88 -1.2 1,557,246 + -0.6 1,557,287 + +1.5
Or see this region's nucleotide sequence