Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01395 and MIT1002_01396 are separated by 128 nucleotides MIT1002_01396 and MIT1002_01397 are separated by 45 nucleotides MIT1002_01397 and MIT1002_01398 are separated by 246 nucleotides MIT1002_01398 and MIT1002_01399 are separated by 37 nucleotides MIT1002_01399 and MIT1002_01400 are separated by 37 nucleotides MIT1002_01400 and MIT1002_01401 are separated by 38 nucleotides
MIT1002_01395: MIT1002_01395 - Cyclic pyranopterin monophosphate synthase, at 1,546,078 to 1,547,046
_01395
MIT1002_01396: MIT1002_01396 - anhydro-N-acetylmuramic acid kinase, at 1,547,175 to 1,547,702
_01396
MIT1002_01397: MIT1002_01397 - DNA-binding transcriptional regulator BasR, at 1,547,748 to 1,549,370
_01397
MIT1002_01398: MIT1002_01398 - tRNA-Val, at 1,549,617 to 1,549,692
_01398
MIT1002_01399: MIT1002_01399 - tRNA-Val, at 1,549,730 to 1,549,805
_01399
MIT1002_01400: MIT1002_01400 - tRNA-Val, at 1,549,843 to 1,549,918
_01400
MIT1002_01401: MIT1002_01401 - tRNA-Val, at 1,549,957 to 1,550,032
_01401
Position (kb)
1547
1548
1549
1550 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1546.774 kb on - strand, within MIT1002_01395 at 1546.844 kb on + strand, within MIT1002_01395 at 1547.034 kb on + strand at 1547.054 kb on + strand at 1547.181 kb on + strand at 1547.286 kb on + strand, within MIT1002_01396 at 1547.340 kb on + strand, within MIT1002_01396 at 1547.341 kb on - strand, within MIT1002_01396 at 1547.355 kb on + strand, within MIT1002_01396 at 1547.355 kb on + strand, within MIT1002_01396 at 1547.355 kb on + strand, within MIT1002_01396 at 1547.362 kb on + strand, within MIT1002_01396 at 1547.363 kb on - strand, within MIT1002_01396 at 1547.380 kb on + strand, within MIT1002_01396 at 1547.421 kb on + strand, within MIT1002_01396 at 1547.450 kb on + strand, within MIT1002_01396 at 1547.450 kb on - strand, within MIT1002_01396 at 1547.501 kb on + strand, within MIT1002_01396 at 1547.501 kb on + strand, within MIT1002_01396 at 1547.507 kb on + strand, within MIT1002_01396 at 1547.509 kb on - strand, within MIT1002_01396 at 1547.515 kb on + strand, within MIT1002_01396 at 1547.515 kb on - strand, within MIT1002_01396 at 1547.515 kb on - strand, within MIT1002_01396 at 1547.557 kb on - strand, within MIT1002_01396 at 1547.557 kb on - strand, within MIT1002_01396 at 1547.661 kb on + strand at 1547.700 kb on - strand at 1547.778 kb on + strand at 1547.864 kb on + strand at 1547.872 kb on - strand at 1547.872 kb on - strand at 1547.965 kb on + strand, within MIT1002_01397 at 1547.965 kb on + strand, within MIT1002_01397 at 1547.965 kb on + strand, within MIT1002_01397 at 1547.973 kb on - strand, within MIT1002_01397 at 1547.973 kb on - strand, within MIT1002_01397 at 1547.989 kb on + strand, within MIT1002_01397 at 1547.989 kb on + strand, within MIT1002_01397 at 1547.989 kb on + strand, within MIT1002_01397 at 1547.989 kb on + strand, within MIT1002_01397 at 1547.997 kb on - strand, within MIT1002_01397 at 1547.997 kb on - strand, within MIT1002_01397 at 1547.997 kb on - strand, within MIT1002_01397 at 1547.997 kb on - strand, within MIT1002_01397 at 1548.007 kb on - strand, within MIT1002_01397 at 1548.073 kb on - strand, within MIT1002_01397 at 1548.121 kb on - strand, within MIT1002_01397 at 1548.199 kb on + strand, within MIT1002_01397 at 1548.262 kb on - strand, within MIT1002_01397 at 1548.320 kb on - strand, within MIT1002_01397 at 1548.322 kb on + strand, within MIT1002_01397 at 1548.389 kb on - strand, within MIT1002_01397 at 1548.509 kb on - strand, within MIT1002_01397 at 1548.509 kb on - strand, within MIT1002_01397 at 1548.567 kb on + strand, within MIT1002_01397 at 1548.593 kb on + strand, within MIT1002_01397 at 1548.601 kb on - strand, within MIT1002_01397 at 1548.603 kb on + strand, within MIT1002_01397 at 1548.604 kb on - strand, within MIT1002_01397 at 1548.606 kb on - strand, within MIT1002_01397 at 1548.606 kb on - strand, within MIT1002_01397 at 1548.606 kb on - strand, within MIT1002_01397 at 1548.611 kb on - strand, within MIT1002_01397 at 1548.623 kb on - strand, within MIT1002_01397 at 1548.662 kb on - strand, within MIT1002_01397 at 1548.683 kb on - strand, within MIT1002_01397 at 1548.812 kb on - strand, within MIT1002_01397 at 1548.814 kb on + strand, within MIT1002_01397 at 1548.815 kb on + strand, within MIT1002_01397 at 1548.855 kb on - strand, within MIT1002_01397 at 1548.892 kb on - strand, within MIT1002_01397 at 1548.923 kb on - strand, within MIT1002_01397 at 1549.012 kb on - strand, within MIT1002_01397 at 1549.064 kb on - strand, within MIT1002_01397 at 1549.087 kb on - strand, within MIT1002_01397 at 1549.124 kb on - strand, within MIT1002_01397 at 1549.228 kb on + strand at 1549.236 kb on - strand at 1549.236 kb on - strand at 1549.306 kb on - strand at 1549.390 kb on + strand at 1549.422 kb on + strand at 1549.433 kb on - strand at 1549.445 kb on + strand at 1549.466 kb on + strand at 1549.526 kb on + strand at 1549.545 kb on + strand at 1549.634 kb on - strand, within MIT1002_01398 at 1549.718 kb on - strand at 1549.721 kb on + strand at 1549.754 kb on - strand, within MIT1002_01399 at 1550.146 kb on + strand at 1550.252 kb on - strand at 1550.266 kb on - strand at 1550.361 kb on + strand at 1550.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 1,546,774 - MIT1002_01395 0.72 -0.6 1,546,844 + MIT1002_01395 0.79 -0.6 1,547,034 + -0.9 1,547,054 + +2.3 1,547,181 + +0.1 1,547,286 + MIT1002_01396 0.21 -0.8 1,547,340 + MIT1002_01396 0.31 +0.3 1,547,341 - MIT1002_01396 0.31 +1.1 1,547,355 + MIT1002_01396 0.34 +1.4 1,547,355 + MIT1002_01396 0.34 +0.7 1,547,355 + MIT1002_01396 0.34 +1.9 1,547,362 + MIT1002_01396 0.35 +0.1 1,547,363 - MIT1002_01396 0.36 -0.2 1,547,380 + MIT1002_01396 0.39 -0.5 1,547,421 + MIT1002_01396 0.47 -0.5 1,547,450 + MIT1002_01396 0.52 -0.6 1,547,450 - MIT1002_01396 0.52 +1.1 1,547,501 + MIT1002_01396 0.62 -0.4 1,547,501 + MIT1002_01396 0.62 -0.0 1,547,507 + MIT1002_01396 0.63 +0.7 1,547,509 - MIT1002_01396 0.63 +1.6 1,547,515 + MIT1002_01396 0.64 +0.3 1,547,515 - MIT1002_01396 0.64 +0.9 1,547,515 - MIT1002_01396 0.64 +0.2 1,547,557 - MIT1002_01396 0.72 +0.7 1,547,557 - MIT1002_01396 0.72 +0.5 1,547,661 + +0.9 1,547,700 - +0.8 1,547,778 + -1.4 1,547,864 + -0.9 1,547,872 - +0.6 1,547,872 - -2.2 1,547,965 + MIT1002_01397 0.13 -1.4 1,547,965 + MIT1002_01397 0.13 -0.7 1,547,965 + MIT1002_01397 0.13 -0.8 1,547,973 - MIT1002_01397 0.14 +0.3 1,547,973 - MIT1002_01397 0.14 +0.8 1,547,989 + MIT1002_01397 0.15 +0.6 1,547,989 + MIT1002_01397 0.15 +1.6 1,547,989 + MIT1002_01397 0.15 -0.5 1,547,989 + MIT1002_01397 0.15 -6.9 1,547,997 - MIT1002_01397 0.15 -0.8 1,547,997 - MIT1002_01397 0.15 +0.5 1,547,997 - MIT1002_01397 0.15 -0.3 1,547,997 - MIT1002_01397 0.15 -1.5 1,548,007 - MIT1002_01397 0.16 -0.5 1,548,073 - MIT1002_01397 0.20 +0.4 1,548,121 - MIT1002_01397 0.23 -0.8 1,548,199 + MIT1002_01397 0.28 -0.4 1,548,262 - MIT1002_01397 0.32 +0.1 1,548,320 - MIT1002_01397 0.35 -0.6 1,548,322 + MIT1002_01397 0.35 +0.1 1,548,389 - MIT1002_01397 0.39 +1.4 1,548,509 - MIT1002_01397 0.47 -0.3 1,548,509 - MIT1002_01397 0.47 +1.0 1,548,567 + MIT1002_01397 0.50 -1.1 1,548,593 + MIT1002_01397 0.52 -0.5 1,548,601 - MIT1002_01397 0.53 +2.3 1,548,603 + MIT1002_01397 0.53 -1.4 1,548,604 - MIT1002_01397 0.53 +0.2 1,548,606 - MIT1002_01397 0.53 -0.8 1,548,606 - MIT1002_01397 0.53 -0.8 1,548,606 - MIT1002_01397 0.53 -1.9 1,548,611 - MIT1002_01397 0.53 -0.2 1,548,623 - MIT1002_01397 0.54 +0.3 1,548,662 - MIT1002_01397 0.56 -0.3 1,548,683 - MIT1002_01397 0.58 -1.1 1,548,812 - MIT1002_01397 0.66 -0.4 1,548,814 + MIT1002_01397 0.66 -0.4 1,548,815 + MIT1002_01397 0.66 +0.9 1,548,855 - MIT1002_01397 0.68 -1.9 1,548,892 - MIT1002_01397 0.70 -1.4 1,548,923 - MIT1002_01397 0.72 +0.5 1,549,012 - MIT1002_01397 0.78 +0.9 1,549,064 - MIT1002_01397 0.81 +0.2 1,549,087 - MIT1002_01397 0.83 +0.6 1,549,124 - MIT1002_01397 0.85 -0.0 1,549,228 + -0.8 1,549,236 - -0.8 1,549,236 - -0.7 1,549,306 - -0.7 1,549,390 + -0.5 1,549,422 + -1.0 1,549,433 - +0.4 1,549,445 + +0.3 1,549,466 + +0.7 1,549,526 + -0.7 1,549,545 + +1.0 1,549,634 - MIT1002_01398 0.22 +0.7 1,549,718 - +0.2 1,549,721 + +1.0 1,549,754 - MIT1002_01399 0.32 -0.2 1,550,146 + -1.3 1,550,252 - -0.3 1,550,266 - -1.6 1,550,361 + -0.4 1,550,369 - -0.3
Or see this region's nucleotide sequence