Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01125 and MIT1002_01126 overlap by 11 nucleotides MIT1002_01126 and MIT1002_01127 are separated by 118 nucleotides MIT1002_01127 and MIT1002_01128 are separated by 297 nucleotides
MIT1002_01125: MIT1002_01125 - Neutral endopeptidase, at 1,236,900 to 1,238,975
_01125
MIT1002_01126: MIT1002_01126 - hypothetical protein, at 1,238,965 to 1,240,290
_01126
MIT1002_01127: MIT1002_01127 - Regulatory protein RecX, at 1,240,409 to 1,240,867
_01127
MIT1002_01128: MIT1002_01128 - Alanine--tRNA ligase, at 1,241,165 to 1,243,762
_01128
Position (kb)
1238
1239
1240
1241 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1237.970 kb on - strand, within MIT1002_01125 at 1238.036 kb on + strand, within MIT1002_01125 at 1238.074 kb on + strand, within MIT1002_01125 at 1238.074 kb on + strand, within MIT1002_01125 at 1238.074 kb on + strand, within MIT1002_01125 at 1238.082 kb on - strand, within MIT1002_01125 at 1238.082 kb on - strand, within MIT1002_01125 at 1238.092 kb on - strand, within MIT1002_01125 at 1238.092 kb on - strand, within MIT1002_01125 at 1238.105 kb on + strand, within MIT1002_01125 at 1238.113 kb on - strand, within MIT1002_01125 at 1238.114 kb on - strand, within MIT1002_01125 at 1238.139 kb on + strand, within MIT1002_01125 at 1238.182 kb on - strand, within MIT1002_01125 at 1238.191 kb on - strand, within MIT1002_01125 at 1238.197 kb on - strand, within MIT1002_01125 at 1238.320 kb on - strand, within MIT1002_01125 at 1238.334 kb on - strand, within MIT1002_01125 at 1238.391 kb on + strand, within MIT1002_01125 at 1238.398 kb on - strand, within MIT1002_01125 at 1238.413 kb on - strand, within MIT1002_01125 at 1238.445 kb on - strand, within MIT1002_01125 at 1238.448 kb on - strand, within MIT1002_01125 at 1238.485 kb on + strand, within MIT1002_01125 at 1238.593 kb on + strand, within MIT1002_01125 at 1238.617 kb on - strand, within MIT1002_01125 at 1238.633 kb on + strand, within MIT1002_01125 at 1238.652 kb on + strand, within MIT1002_01125 at 1238.652 kb on - strand, within MIT1002_01125 at 1238.762 kb on + strand, within MIT1002_01125 at 1238.770 kb on - strand at 1238.823 kb on + strand at 1238.849 kb on + strand at 1238.876 kb on + strand at 1238.938 kb on - strand at 1239.009 kb on - strand at 1239.063 kb on + strand at 1239.320 kb on + strand, within MIT1002_01126 at 1239.334 kb on + strand, within MIT1002_01126 at 1239.342 kb on - strand, within MIT1002_01126 at 1239.370 kb on + strand, within MIT1002_01126 at 1239.402 kb on + strand, within MIT1002_01126 at 1239.412 kb on - strand, within MIT1002_01126 at 1239.497 kb on + strand, within MIT1002_01126 at 1239.497 kb on + strand, within MIT1002_01126 at 1239.621 kb on + strand, within MIT1002_01126 at 1239.634 kb on + strand, within MIT1002_01126 at 1239.676 kb on + strand, within MIT1002_01126 at 1239.699 kb on + strand, within MIT1002_01126 at 1239.706 kb on - strand, within MIT1002_01126 at 1239.768 kb on + strand, within MIT1002_01126 at 1239.785 kb on - strand, within MIT1002_01126 at 1239.842 kb on + strand, within MIT1002_01126 at 1239.844 kb on - strand, within MIT1002_01126 at 1239.927 kb on - strand, within MIT1002_01126 at 1239.976 kb on - strand, within MIT1002_01126 at 1240.005 kb on - strand, within MIT1002_01126 at 1240.013 kb on + strand, within MIT1002_01126 at 1240.013 kb on + strand, within MIT1002_01126 at 1240.013 kb on + strand, within MIT1002_01126 at 1240.013 kb on + strand, within MIT1002_01126 at 1240.021 kb on - strand, within MIT1002_01126 at 1240.021 kb on - strand, within MIT1002_01126 at 1240.021 kb on - strand, within MIT1002_01126 at 1240.021 kb on - strand, within MIT1002_01126 at 1240.021 kb on - strand, within MIT1002_01126 at 1240.038 kb on + strand, within MIT1002_01126 at 1240.091 kb on + strand, within MIT1002_01126 at 1240.103 kb on - strand, within MIT1002_01126 at 1240.164 kb on + strand at 1240.172 kb on - strand at 1240.223 kb on + strand at 1240.233 kb on + strand at 1240.233 kb on + strand at 1240.233 kb on + strand at 1240.241 kb on - strand at 1240.286 kb on + strand at 1240.286 kb on + strand at 1240.294 kb on - strand at 1240.318 kb on - strand at 1240.326 kb on + strand at 1240.333 kb on - strand at 1240.469 kb on + strand, within MIT1002_01127 at 1240.476 kb on + strand, within MIT1002_01127 at 1240.477 kb on - strand, within MIT1002_01127 at 1240.493 kb on - strand, within MIT1002_01127 at 1240.660 kb on - strand, within MIT1002_01127 at 1240.660 kb on - strand, within MIT1002_01127 at 1240.665 kb on - strand, within MIT1002_01127 at 1240.667 kb on + strand, within MIT1002_01127 at 1240.675 kb on - strand, within MIT1002_01127 at 1240.677 kb on + strand, within MIT1002_01127 at 1240.685 kb on - strand, within MIT1002_01127 at 1240.707 kb on - strand, within MIT1002_01127 at 1240.747 kb on - strand, within MIT1002_01127 at 1240.810 kb on - strand, within MIT1002_01127 at 1240.865 kb on - strand at 1240.875 kb on + strand at 1240.875 kb on + strand at 1240.934 kb on + strand at 1240.979 kb on + strand at 1240.979 kb on + strand at 1240.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 1,237,970 - MIT1002_01125 0.52 -0.1 1,238,036 + MIT1002_01125 0.55 +0.1 1,238,074 + MIT1002_01125 0.57 -1.7 1,238,074 + MIT1002_01125 0.57 -0.3 1,238,074 + MIT1002_01125 0.57 -0.7 1,238,082 - MIT1002_01125 0.57 +1.0 1,238,082 - MIT1002_01125 0.57 +2.0 1,238,092 - MIT1002_01125 0.57 -0.3 1,238,092 - MIT1002_01125 0.57 +0.8 1,238,105 + MIT1002_01125 0.58 +1.3 1,238,113 - MIT1002_01125 0.58 -0.2 1,238,114 - MIT1002_01125 0.58 -0.0 1,238,139 + MIT1002_01125 0.60 +0.5 1,238,182 - MIT1002_01125 0.62 -0.5 1,238,191 - MIT1002_01125 0.62 -1.4 1,238,197 - MIT1002_01125 0.62 +1.6 1,238,320 - MIT1002_01125 0.68 -0.8 1,238,334 - MIT1002_01125 0.69 -1.3 1,238,391 + MIT1002_01125 0.72 -0.6 1,238,398 - MIT1002_01125 0.72 -1.9 1,238,413 - MIT1002_01125 0.73 +0.1 1,238,445 - MIT1002_01125 0.74 -2.6 1,238,448 - MIT1002_01125 0.75 -0.3 1,238,485 + MIT1002_01125 0.76 +0.4 1,238,593 + MIT1002_01125 0.82 -1.9 1,238,617 - MIT1002_01125 0.83 +0.8 1,238,633 + MIT1002_01125 0.83 +0.4 1,238,652 + MIT1002_01125 0.84 +1.2 1,238,652 - MIT1002_01125 0.84 -0.0 1,238,762 + MIT1002_01125 0.90 -0.2 1,238,770 - -0.1 1,238,823 + -1.1 1,238,849 + +1.0 1,238,876 + -0.6 1,238,938 - +0.9 1,239,009 - -0.5 1,239,063 + +0.0 1,239,320 + MIT1002_01126 0.27 -0.1 1,239,334 + MIT1002_01126 0.28 -0.0 1,239,342 - MIT1002_01126 0.28 -1.2 1,239,370 + MIT1002_01126 0.31 -0.5 1,239,402 + MIT1002_01126 0.33 +0.3 1,239,412 - MIT1002_01126 0.34 +1.6 1,239,497 + MIT1002_01126 0.40 +0.2 1,239,497 + MIT1002_01126 0.40 -0.7 1,239,621 + MIT1002_01126 0.49 +0.7 1,239,634 + MIT1002_01126 0.50 -0.2 1,239,676 + MIT1002_01126 0.54 +0.2 1,239,699 + MIT1002_01126 0.55 -0.8 1,239,706 - MIT1002_01126 0.56 +2.4 1,239,768 + MIT1002_01126 0.61 -2.3 1,239,785 - MIT1002_01126 0.62 +0.1 1,239,842 + MIT1002_01126 0.66 +0.1 1,239,844 - MIT1002_01126 0.66 +0.9 1,239,927 - MIT1002_01126 0.73 +0.7 1,239,976 - MIT1002_01126 0.76 +2.3 1,240,005 - MIT1002_01126 0.78 +0.5 1,240,013 + MIT1002_01126 0.79 -1.1 1,240,013 + MIT1002_01126 0.79 +0.3 1,240,013 + MIT1002_01126 0.79 +3.4 1,240,013 + MIT1002_01126 0.79 +1.0 1,240,021 - MIT1002_01126 0.80 +0.2 1,240,021 - MIT1002_01126 0.80 +0.3 1,240,021 - MIT1002_01126 0.80 -0.1 1,240,021 - MIT1002_01126 0.80 -0.1 1,240,021 - MIT1002_01126 0.80 -1.7 1,240,038 + MIT1002_01126 0.81 +0.7 1,240,091 + MIT1002_01126 0.85 +0.5 1,240,103 - MIT1002_01126 0.86 -2.5 1,240,164 + +0.6 1,240,172 - -1.2 1,240,223 + +0.4 1,240,233 + +1.2 1,240,233 + +0.5 1,240,233 + -0.1 1,240,241 - -0.2 1,240,286 + +0.9 1,240,286 + +0.9 1,240,294 - -0.7 1,240,318 - -0.1 1,240,326 + -0.1 1,240,333 - -1.3 1,240,469 + MIT1002_01127 0.13 -0.4 1,240,476 + MIT1002_01127 0.15 +0.6 1,240,477 - MIT1002_01127 0.15 -0.7 1,240,493 - MIT1002_01127 0.18 +1.4 1,240,660 - MIT1002_01127 0.55 -0.4 1,240,660 - MIT1002_01127 0.55 -0.5 1,240,665 - MIT1002_01127 0.56 +0.1 1,240,667 + MIT1002_01127 0.56 +0.8 1,240,675 - MIT1002_01127 0.58 -0.1 1,240,677 + MIT1002_01127 0.58 -0.5 1,240,685 - MIT1002_01127 0.60 +1.4 1,240,707 - MIT1002_01127 0.65 -0.3 1,240,747 - MIT1002_01127 0.74 -1.2 1,240,810 - MIT1002_01127 0.87 -0.6 1,240,865 - -0.2 1,240,875 + -1.4 1,240,875 + +0.1 1,240,934 + +2.6 1,240,979 + +0.9 1,240,979 + -1.0 1,240,987 - +2.9
Or see this region's nucleotide sequence