Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00997 and MIT1002_00998 are separated by 16 nucleotides MIT1002_00998 and MIT1002_00999 are separated by 66 nucleotides MIT1002_00999 and MIT1002_01000 are separated by 130 nucleotides MIT1002_01000 and MIT1002_01001 overlap by 4 nucleotides
MIT1002_00997: MIT1002_00997 - hypothetical protein, at 1,104,656 to 1,105,000
_00997
MIT1002_00998: MIT1002_00998 - tRNA pseudouridine synthase C, at 1,105,017 to 1,105,832
_00998
MIT1002_00999: MIT1002_00999 - Sulfite reductase [NADPH] flavoprotein alpha-component, at 1,105,899 to 1,106,348
_00999
MIT1002_01000: MIT1002_01000 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,106,479 to 1,107,303
_01000
MIT1002_01001: MIT1002_01001 - PII uridylyl-transferase, at 1,107,300 to 1,109,951
_01001
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1105.012 kb on + strand at 1105.017 kb on + strand at 1105.017 kb on + strand at 1105.025 kb on - strand at 1105.108 kb on - strand, within MIT1002_00998 at 1105.122 kb on + strand, within MIT1002_00998 at 1105.134 kb on + strand, within MIT1002_00998 at 1105.135 kb on - strand, within MIT1002_00998 at 1105.168 kb on + strand, within MIT1002_00998 at 1105.176 kb on + strand, within MIT1002_00998 at 1105.181 kb on - strand, within MIT1002_00998 at 1105.218 kb on + strand, within MIT1002_00998 at 1105.218 kb on + strand, within MIT1002_00998 at 1105.225 kb on + strand, within MIT1002_00998 at 1105.226 kb on - strand, within MIT1002_00998 at 1105.250 kb on + strand, within MIT1002_00998 at 1105.250 kb on + strand, within MIT1002_00998 at 1105.250 kb on + strand, within MIT1002_00998 at 1105.250 kb on + strand, within MIT1002_00998 at 1105.250 kb on - strand, within MIT1002_00998 at 1105.282 kb on + strand, within MIT1002_00998 at 1105.395 kb on - strand, within MIT1002_00998 at 1105.404 kb on + strand, within MIT1002_00998 at 1105.404 kb on + strand, within MIT1002_00998 at 1105.404 kb on + strand, within MIT1002_00998 at 1105.407 kb on - strand, within MIT1002_00998 at 1105.428 kb on - strand, within MIT1002_00998 at 1105.531 kb on - strand, within MIT1002_00998 at 1105.531 kb on - strand, within MIT1002_00998 at 1105.556 kb on + strand, within MIT1002_00998 at 1105.556 kb on + strand, within MIT1002_00998 at 1105.590 kb on - strand, within MIT1002_00998 at 1105.710 kb on + strand, within MIT1002_00998 at 1105.929 kb on + strand at 1105.929 kb on + strand at 1105.954 kb on - strand, within MIT1002_00999 at 1105.959 kb on - strand, within MIT1002_00999 at 1105.975 kb on + strand, within MIT1002_00999 at 1105.985 kb on - strand, within MIT1002_00999 at 1106.009 kb on - strand, within MIT1002_00999 at 1106.046 kb on + strand, within MIT1002_00999 at 1106.068 kb on - strand, within MIT1002_00999 at 1106.070 kb on + strand, within MIT1002_00999 at 1106.070 kb on + strand, within MIT1002_00999 at 1106.133 kb on + strand, within MIT1002_00999 at 1106.163 kb on + strand, within MIT1002_00999 at 1106.163 kb on + strand, within MIT1002_00999 at 1106.183 kb on + strand, within MIT1002_00999 at 1106.242 kb on + strand, within MIT1002_00999 at 1106.267 kb on - strand, within MIT1002_00999 at 1106.284 kb on + strand, within MIT1002_00999 at 1106.315 kb on + strand at 1106.391 kb on - strand at 1106.489 kb on - strand at 1106.512 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 1,105,012 + +0.7 1,105,017 + -0.1 1,105,017 + -0.1 1,105,025 - -0.0 1,105,108 - MIT1002_00998 0.11 -0.4 1,105,122 + MIT1002_00998 0.13 +0.7 1,105,134 + MIT1002_00998 0.14 -0.4 1,105,135 - MIT1002_00998 0.14 +0.3 1,105,168 + MIT1002_00998 0.19 +0.1 1,105,176 + MIT1002_00998 0.19 +0.3 1,105,181 - MIT1002_00998 0.20 +3.4 1,105,218 + MIT1002_00998 0.25 -0.6 1,105,218 + MIT1002_00998 0.25 +1.3 1,105,225 + MIT1002_00998 0.25 -0.5 1,105,226 - MIT1002_00998 0.26 +0.5 1,105,250 + MIT1002_00998 0.29 -0.9 1,105,250 + MIT1002_00998 0.29 +0.4 1,105,250 + MIT1002_00998 0.29 -0.2 1,105,250 + MIT1002_00998 0.29 -0.5 1,105,250 - MIT1002_00998 0.29 +0.3 1,105,282 + MIT1002_00998 0.32 -0.3 1,105,395 - MIT1002_00998 0.46 +0.3 1,105,404 + MIT1002_00998 0.47 -0.1 1,105,404 + MIT1002_00998 0.47 -0.7 1,105,404 + MIT1002_00998 0.47 +1.3 1,105,407 - MIT1002_00998 0.48 +0.2 1,105,428 - MIT1002_00998 0.50 -0.2 1,105,531 - MIT1002_00998 0.63 -0.3 1,105,531 - MIT1002_00998 0.63 -0.9 1,105,556 + MIT1002_00998 0.66 -1.3 1,105,556 + MIT1002_00998 0.66 +0.4 1,105,590 - MIT1002_00998 0.70 +0.5 1,105,710 + MIT1002_00998 0.85 +0.0 1,105,929 + -0.5 1,105,929 + -1.1 1,105,954 - MIT1002_00999 0.12 -0.6 1,105,959 - MIT1002_00999 0.13 -0.4 1,105,975 + MIT1002_00999 0.17 +0.3 1,105,985 - MIT1002_00999 0.19 -0.3 1,106,009 - MIT1002_00999 0.24 -0.8 1,106,046 + MIT1002_00999 0.33 -1.2 1,106,068 - MIT1002_00999 0.38 +0.5 1,106,070 + MIT1002_00999 0.38 +0.7 1,106,070 + MIT1002_00999 0.38 -0.5 1,106,133 + MIT1002_00999 0.52 +0.1 1,106,163 + MIT1002_00999 0.59 -1.2 1,106,163 + MIT1002_00999 0.59 -1.3 1,106,183 + MIT1002_00999 0.63 -0.2 1,106,242 + MIT1002_00999 0.76 -0.6 1,106,267 - MIT1002_00999 0.82 -0.6 1,106,284 + MIT1002_00999 0.86 +1.1 1,106,315 + +0.2 1,106,391 - +1.1 1,106,489 - +0.7 1,106,512 - -0.3
Or see this region's nucleotide sequence