Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00729 and MIT1002_00730 are separated by 530 nucleotides MIT1002_00730 and MIT1002_00731 are separated by 34 nucleotides MIT1002_00731 and MIT1002_00732 are separated by 143 nucleotides
MIT1002_00729: MIT1002_00729 - putative efflux pump membrane transporter TtgB, at 797,233 to 800,340
_00729
MIT1002_00730: MIT1002_00730 - phosphate binding protein, at 800,871 to 801,275
_00730
MIT1002_00731: MIT1002_00731 - hypothetical protein, at 801,310 to 802,509
_00731
MIT1002_00732: MIT1002_00732 - Methyl-accepting chemotaxis protein 4, at 802,653 to 804,272
_00732
Position (kb) 801
802
803 Strain fitness (log2 ratio) -2
-1
0
1
2 at 800.310 kb on + strand at 800.350 kb on + strand at 800.358 kb on - strand at 800.365 kb on - strand at 800.424 kb on + strand at 800.618 kb on + strand at 800.626 kb on - strand at 800.626 kb on - strand at 800.626 kb on - strand at 800.629 kb on + strand at 800.629 kb on + strand at 800.637 kb on - strand at 800.637 kb on - strand at 800.637 kb on - strand at 800.732 kb on - strand at 800.851 kb on - strand at 800.863 kb on - strand at 800.865 kb on - strand at 800.903 kb on - strand at 801.045 kb on + strand, within MIT1002_00730 at 801.092 kb on - strand, within MIT1002_00730 at 801.092 kb on - strand, within MIT1002_00730 at 801.156 kb on - strand, within MIT1002_00730 at 801.165 kb on - strand, within MIT1002_00730 at 801.175 kb on + strand, within MIT1002_00730 at 801.233 kb on - strand, within MIT1002_00730 at 801.240 kb on + strand at 801.240 kb on + strand at 801.246 kb on - strand at 801.248 kb on - strand at 801.248 kb on - strand at 801.250 kb on + strand at 801.348 kb on + strand at 801.356 kb on + strand at 801.404 kb on - strand at 801.404 kb on - strand at 801.465 kb on - strand, within MIT1002_00731 at 801.595 kb on - strand, within MIT1002_00731 at 801.595 kb on - strand, within MIT1002_00731 at 801.686 kb on + strand, within MIT1002_00731 at 801.704 kb on - strand, within MIT1002_00731 at 801.737 kb on - strand, within MIT1002_00731 at 801.737 kb on - strand, within MIT1002_00731 at 801.759 kb on + strand, within MIT1002_00731 at 801.767 kb on - strand, within MIT1002_00731 at 801.769 kb on - strand, within MIT1002_00731 at 801.787 kb on - strand, within MIT1002_00731 at 801.789 kb on + strand, within MIT1002_00731 at 801.789 kb on + strand, within MIT1002_00731 at 801.789 kb on + strand, within MIT1002_00731 at 801.810 kb on + strand, within MIT1002_00731 at 801.968 kb on + strand, within MIT1002_00731 at 802.020 kb on + strand, within MIT1002_00731 at 802.094 kb on + strand, within MIT1002_00731 at 802.147 kb on + strand, within MIT1002_00731 at 802.147 kb on + strand, within MIT1002_00731 at 802.182 kb on + strand, within MIT1002_00731 at 802.361 kb on + strand, within MIT1002_00731 at 802.376 kb on + strand, within MIT1002_00731 at 802.432 kb on - strand at 802.469 kb on + strand at 802.477 kb on - strand at 802.479 kb on + strand at 802.479 kb on + strand at 802.511 kb on - strand at 802.768 kb on - strand at 802.848 kb on - strand, within MIT1002_00732 at 802.892 kb on - strand, within MIT1002_00732 at 802.960 kb on + strand, within MIT1002_00732 at 803.022 kb on + strand, within MIT1002_00732 at 803.029 kb on + strand, within MIT1002_00732 at 803.038 kb on + strand, within MIT1002_00732 at 803.039 kb on - strand, within MIT1002_00732 at 803.084 kb on - strand, within MIT1002_00732 at 803.102 kb on + strand, within MIT1002_00732 at 803.112 kb on + strand, within MIT1002_00732 at 803.153 kb on - strand, within MIT1002_00732 at 803.156 kb on - strand, within MIT1002_00732 at 803.197 kb on - strand, within MIT1002_00732 at 803.429 kb on - strand, within MIT1002_00732 at 803.473 kb on - strand, within MIT1002_00732 at 803.499 kb on + strand, within MIT1002_00732 at 803.499 kb on - strand, within MIT1002_00732
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 800,310 + -0.4 800,350 + -0.2 800,358 - -0.1 800,365 - -0.2 800,424 + -0.8 800,618 + +0.5 800,626 - -1.9 800,626 - -0.3 800,626 - -1.1 800,629 + -1.4 800,629 + -1.0 800,637 - -0.1 800,637 - +0.5 800,637 - +0.1 800,732 - +0.5 800,851 - +0.7 800,863 - -0.2 800,865 - -1.5 800,903 - +0.4 801,045 + MIT1002_00730 0.43 -0.1 801,092 - MIT1002_00730 0.55 +0.6 801,092 - MIT1002_00730 0.55 +0.7 801,156 - MIT1002_00730 0.70 -2.4 801,165 - MIT1002_00730 0.73 -0.3 801,175 + MIT1002_00730 0.75 -0.3 801,233 - MIT1002_00730 0.89 -0.2 801,240 + -1.0 801,240 + -0.9 801,246 - -1.2 801,248 - +1.5 801,248 - +0.4 801,250 + -0.5 801,348 + +0.6 801,356 + +0.0 801,404 - -0.5 801,404 - +0.1 801,465 - MIT1002_00731 0.13 -0.7 801,595 - MIT1002_00731 0.24 -0.5 801,595 - MIT1002_00731 0.24 +0.6 801,686 + MIT1002_00731 0.31 -0.0 801,704 - MIT1002_00731 0.33 +0.4 801,737 - MIT1002_00731 0.36 +0.6 801,737 - MIT1002_00731 0.36 +1.4 801,759 + MIT1002_00731 0.37 -0.2 801,767 - MIT1002_00731 0.38 -0.7 801,769 - MIT1002_00731 0.38 +0.4 801,787 - MIT1002_00731 0.40 +2.6 801,789 + MIT1002_00731 0.40 -1.2 801,789 + MIT1002_00731 0.40 -1.7 801,789 + MIT1002_00731 0.40 +0.2 801,810 + MIT1002_00731 0.42 +1.1 801,968 + MIT1002_00731 0.55 -1.4 802,020 + MIT1002_00731 0.59 -0.5 802,094 + MIT1002_00731 0.65 -1.0 802,147 + MIT1002_00731 0.70 +0.1 802,147 + MIT1002_00731 0.70 +0.4 802,182 + MIT1002_00731 0.73 -0.2 802,361 + MIT1002_00731 0.88 -2.5 802,376 + MIT1002_00731 0.89 +1.0 802,432 - -0.5 802,469 + -1.1 802,477 - -0.3 802,479 + -0.2 802,479 + +0.2 802,511 - +0.4 802,768 - -0.4 802,848 - MIT1002_00732 0.12 -0.1 802,892 - MIT1002_00732 0.15 -1.7 802,960 + MIT1002_00732 0.19 -0.5 803,022 + MIT1002_00732 0.23 -0.8 803,029 + MIT1002_00732 0.23 +1.3 803,038 + MIT1002_00732 0.24 +0.0 803,039 - MIT1002_00732 0.24 -0.2 803,084 - MIT1002_00732 0.27 -1.0 803,102 + MIT1002_00732 0.28 -0.4 803,112 + MIT1002_00732 0.28 +0.8 803,153 - MIT1002_00732 0.31 +1.8 803,156 - MIT1002_00732 0.31 +0.9 803,197 - MIT1002_00732 0.34 +1.0 803,429 - MIT1002_00732 0.48 -0.0 803,473 - MIT1002_00732 0.51 -0.4 803,499 + MIT1002_00732 0.52 -0.3 803,499 - MIT1002_00732 0.52 -0.4
Or see this region's nucleotide sequence