Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00625 and MIT1002_00626 are separated by 72 nucleotides MIT1002_00626 and MIT1002_00627 are separated by 1 nucleotides MIT1002_00627 and MIT1002_00628 overlap by 4 nucleotides
MIT1002_00625: MIT1002_00625 - RNA polymerase sigma factor RpoS, at 673,830 to 674,822
_00625
MIT1002_00626: MIT1002_00626 - Murein hydrolase activator NlpD precursor, at 674,895 to 675,764
_00626
MIT1002_00627: MIT1002_00627 - Inner membrane protein YqaA, at 675,766 to 676,344
_00627
MIT1002_00628: MIT1002_00628 - Protein-L-isoaspartate O-methyltransferase, at 676,341 to 676,976
_00628
Position (kb) 674
675
676 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 673.976 kb on + strand, within MIT1002_00625 at 673.977 kb on - strand, within MIT1002_00625 at 673.985 kb on + strand, within MIT1002_00625 at 673.999 kb on - strand, within MIT1002_00625 at 674.021 kb on + strand, within MIT1002_00625 at 674.034 kb on - strand, within MIT1002_00625 at 674.054 kb on - strand, within MIT1002_00625 at 674.083 kb on - strand, within MIT1002_00625 at 674.085 kb on - strand, within MIT1002_00625 at 674.087 kb on + strand, within MIT1002_00625 at 674.087 kb on + strand, within MIT1002_00625 at 674.090 kb on - strand, within MIT1002_00625 at 674.094 kb on + strand, within MIT1002_00625 at 674.114 kb on + strand, within MIT1002_00625 at 674.121 kb on + strand, within MIT1002_00625 at 674.309 kb on - strand, within MIT1002_00625 at 674.340 kb on - strand, within MIT1002_00625 at 674.349 kb on + strand, within MIT1002_00625 at 674.357 kb on - strand, within MIT1002_00625 at 674.438 kb on - strand, within MIT1002_00625 at 674.522 kb on + strand, within MIT1002_00625 at 674.523 kb on - strand, within MIT1002_00625 at 674.552 kb on + strand, within MIT1002_00625 at 674.644 kb on + strand, within MIT1002_00625 at 674.652 kb on - strand, within MIT1002_00625 at 674.652 kb on - strand, within MIT1002_00625 at 674.659 kb on + strand, within MIT1002_00625 at 674.667 kb on + strand, within MIT1002_00625 at 674.719 kb on + strand, within MIT1002_00625 at 674.789 kb on - strand at 674.789 kb on - strand at 674.803 kb on + strand at 674.946 kb on - strand at 674.993 kb on - strand, within MIT1002_00626 at 675.013 kb on + strand, within MIT1002_00626 at 675.021 kb on - strand, within MIT1002_00626 at 675.025 kb on - strand, within MIT1002_00626 at 675.032 kb on - strand, within MIT1002_00626 at 675.173 kb on + strand, within MIT1002_00626 at 675.235 kb on + strand, within MIT1002_00626 at 675.258 kb on - strand, within MIT1002_00626 at 675.258 kb on - strand, within MIT1002_00626 at 675.258 kb on - strand, within MIT1002_00626 at 675.261 kb on + strand, within MIT1002_00626 at 675.261 kb on + strand, within MIT1002_00626 at 675.269 kb on - strand, within MIT1002_00626 at 675.450 kb on - strand, within MIT1002_00626 at 675.450 kb on - strand, within MIT1002_00626 at 675.584 kb on - strand, within MIT1002_00626 at 675.586 kb on + strand, within MIT1002_00626 at 675.600 kb on + strand, within MIT1002_00626 at 675.754 kb on + strand at 675.830 kb on - strand, within MIT1002_00627 at 675.832 kb on - strand, within MIT1002_00627 at 676.029 kb on + strand, within MIT1002_00627 at 676.029 kb on + strand, within MIT1002_00627 at 676.314 kb on - strand at 676.314 kb on - strand at 676.352 kb on - strand at 676.502 kb on + strand, within MIT1002_00628 at 676.655 kb on + strand, within MIT1002_00628 at 676.655 kb on + strand, within MIT1002_00628 at 676.663 kb on - strand, within MIT1002_00628 at 676.701 kb on + strand, within MIT1002_00628 at 676.715 kb on - strand, within MIT1002_00628
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 673,976 + MIT1002_00625 0.15 -2.3 673,977 - MIT1002_00625 0.15 -1.5 673,985 + MIT1002_00625 0.16 -1.0 673,999 - MIT1002_00625 0.17 -2.2 674,021 + MIT1002_00625 0.19 -2.4 674,034 - MIT1002_00625 0.21 -0.3 674,054 - MIT1002_00625 0.23 -0.9 674,083 - MIT1002_00625 0.25 -0.8 674,085 - MIT1002_00625 0.26 -1.1 674,087 + MIT1002_00625 0.26 -1.9 674,087 + MIT1002_00625 0.26 -1.4 674,090 - MIT1002_00625 0.26 -0.2 674,094 + MIT1002_00625 0.27 -1.4 674,114 + MIT1002_00625 0.29 -1.8 674,121 + MIT1002_00625 0.29 -1.2 674,309 - MIT1002_00625 0.48 -1.7 674,340 - MIT1002_00625 0.51 -2.0 674,349 + MIT1002_00625 0.52 +1.9 674,357 - MIT1002_00625 0.53 +1.4 674,438 - MIT1002_00625 0.61 +0.1 674,522 + MIT1002_00625 0.70 -0.9 674,523 - MIT1002_00625 0.70 -0.5 674,552 + MIT1002_00625 0.73 +1.1 674,644 + MIT1002_00625 0.82 +0.0 674,652 - MIT1002_00625 0.83 -0.8 674,652 - MIT1002_00625 0.83 -1.2 674,659 + MIT1002_00625 0.83 -1.3 674,667 + MIT1002_00625 0.84 -0.1 674,719 + MIT1002_00625 0.90 -1.7 674,789 - -0.8 674,789 - +0.5 674,803 + -1.7 674,946 - -2.5 674,993 - MIT1002_00626 0.11 -0.0 675,013 + MIT1002_00626 0.14 -1.4 675,021 - MIT1002_00626 0.14 -3.0 675,025 - MIT1002_00626 0.15 -1.9 675,032 - MIT1002_00626 0.16 -1.3 675,173 + MIT1002_00626 0.32 -2.3 675,235 + MIT1002_00626 0.39 -2.0 675,258 - MIT1002_00626 0.42 -1.8 675,258 - MIT1002_00626 0.42 -1.4 675,258 - MIT1002_00626 0.42 -1.6 675,261 + MIT1002_00626 0.42 -1.3 675,261 + MIT1002_00626 0.42 -2.2 675,269 - MIT1002_00626 0.43 -0.8 675,450 - MIT1002_00626 0.64 +0.2 675,450 - MIT1002_00626 0.64 -1.5 675,584 - MIT1002_00626 0.79 -0.9 675,586 + MIT1002_00626 0.79 -0.2 675,600 + MIT1002_00626 0.81 -0.5 675,754 + -1.2 675,830 - MIT1002_00627 0.11 -0.7 675,832 - MIT1002_00627 0.11 +0.0 676,029 + MIT1002_00627 0.45 -1.5 676,029 + MIT1002_00627 0.45 -2.3 676,314 - +1.9 676,314 - +0.5 676,352 - -0.1 676,502 + MIT1002_00628 0.25 -0.9 676,655 + MIT1002_00628 0.49 -1.2 676,655 + MIT1002_00628 0.49 -0.5 676,663 - MIT1002_00628 0.51 +0.8 676,701 + MIT1002_00628 0.57 -0.1 676,715 - MIT1002_00628 0.59 -1.2
Or see this region's nucleotide sequence