Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00397 and MIT1002_00398 are separated by 241 nucleotides MIT1002_00398 and MIT1002_00399 are separated by 21 nucleotides MIT1002_00399 and MIT1002_00400 are separated by 60 nucleotides MIT1002_00400 and MIT1002_00401 are separated by 408 nucleotides
MIT1002_00397: MIT1002_00397 - hypothetical protein, at 426,034 to 426,885
_00397
MIT1002_00398: MIT1002_00398 - hypothetical protein, at 427,127 to 427,384
_00398
MIT1002_00399: MIT1002_00399 - hypothetical protein, at 427,406 to 427,714
_00399
MIT1002_00400: MIT1002_00400 - hypothetical protein, at 427,775 to 429,685
_00400
MIT1002_00401: MIT1002_00401 - Peptidyl-dipeptidase dcp, at 430,094 to 432,283
_00401
Position (kb)
427
428
429
430 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 426.836 kb on + strand at 426.844 kb on - strand at 426.895 kb on + strand at 426.948 kb on + strand at 426.981 kb on + strand at 427.002 kb on + strand at 427.043 kb on + strand at 427.065 kb on - strand at 427.069 kb on + strand at 427.092 kb on + strand at 427.228 kb on - strand, within MIT1002_00398 at 427.365 kb on + strand at 427.439 kb on + strand, within MIT1002_00399 at 427.475 kb on + strand, within MIT1002_00399 at 427.520 kb on - strand, within MIT1002_00399 at 427.633 kb on + strand, within MIT1002_00399 at 427.672 kb on - strand, within MIT1002_00399 at 427.673 kb on - strand, within MIT1002_00399 at 427.757 kb on + strand at 427.780 kb on + strand at 427.780 kb on + strand at 427.788 kb on - strand at 427.795 kb on + strand at 427.803 kb on - strand at 427.968 kb on + strand, within MIT1002_00400 at 427.976 kb on - strand, within MIT1002_00400 at 427.976 kb on - strand, within MIT1002_00400 at 428.090 kb on - strand, within MIT1002_00400 at 428.111 kb on + strand, within MIT1002_00400 at 428.119 kb on + strand, within MIT1002_00400 at 428.128 kb on - strand, within MIT1002_00400 at 428.149 kb on + strand, within MIT1002_00400 at 428.157 kb on - strand, within MIT1002_00400 at 428.162 kb on + strand, within MIT1002_00400 at 428.298 kb on - strand, within MIT1002_00400 at 428.300 kb on - strand, within MIT1002_00400 at 428.308 kb on - strand, within MIT1002_00400 at 428.347 kb on - strand, within MIT1002_00400 at 428.444 kb on - strand, within MIT1002_00400 at 428.448 kb on - strand, within MIT1002_00400 at 428.509 kb on - strand, within MIT1002_00400 at 428.515 kb on - strand, within MIT1002_00400 at 428.576 kb on + strand, within MIT1002_00400 at 428.708 kb on + strand, within MIT1002_00400 at 428.716 kb on - strand, within MIT1002_00400 at 428.757 kb on + strand, within MIT1002_00400 at 428.844 kb on + strand, within MIT1002_00400 at 428.859 kb on + strand, within MIT1002_00400 at 428.867 kb on - strand, within MIT1002_00400 at 428.872 kb on - strand, within MIT1002_00400 at 428.980 kb on - strand, within MIT1002_00400 at 428.989 kb on - strand, within MIT1002_00400 at 428.992 kb on + strand, within MIT1002_00400 at 428.992 kb on + strand, within MIT1002_00400 at 429.048 kb on + strand, within MIT1002_00400 at 429.048 kb on + strand, within MIT1002_00400 at 429.056 kb on - strand, within MIT1002_00400 at 429.056 kb on - strand, within MIT1002_00400 at 429.137 kb on - strand, within MIT1002_00400 at 429.205 kb on + strand, within MIT1002_00400 at 429.213 kb on + strand, within MIT1002_00400 at 429.221 kb on - strand, within MIT1002_00400 at 429.221 kb on - strand, within MIT1002_00400 at 429.243 kb on - strand, within MIT1002_00400 at 429.416 kb on + strand, within MIT1002_00400 at 429.424 kb on - strand, within MIT1002_00400 at 429.480 kb on - strand, within MIT1002_00400 at 429.489 kb on + strand, within MIT1002_00400 at 429.496 kb on + strand at 429.496 kb on + strand at 429.504 kb on - strand at 429.510 kb on + strand at 429.510 kb on + strand at 429.513 kb on - strand at 429.532 kb on - strand at 429.727 kb on + strand at 429.768 kb on + strand at 429.776 kb on - strand at 429.776 kb on - strand at 429.776 kb on - strand at 429.776 kb on - strand at 429.778 kb on + strand at 429.778 kb on + strand at 429.778 kb on + strand at 429.778 kb on + strand at 429.778 kb on + strand at 429.778 kb on + strand at 429.783 kb on + strand at 429.783 kb on + strand at 429.786 kb on - strand at 429.791 kb on - strand at 429.791 kb on - strand at 429.868 kb on - strand at 429.887 kb on - strand at 430.026 kb on + strand at 430.054 kb on - strand at 430.078 kb on + strand at 430.078 kb on + strand at 430.086 kb on - strand at 430.086 kb on - strand at 430.091 kb on + strand at 430.190 kb on - strand at 430.280 kb on + strand at 430.280 kb on + strand at 430.280 kb on + strand at 430.280 kb on + strand at 430.288 kb on - strand at 430.314 kb on - strand, within MIT1002_00401 at 430.316 kb on + strand, within MIT1002_00401 at 430.316 kb on + strand, within MIT1002_00401 at 430.333 kb on - strand, within MIT1002_00401 at 430.338 kb on + strand, within MIT1002_00401 at 430.338 kb on + strand, within MIT1002_00401 at 430.338 kb on + strand, within MIT1002_00401 at 430.343 kb on + strand, within MIT1002_00401 at 430.346 kb on - strand, within MIT1002_00401 at 430.346 kb on - strand, within MIT1002_00401 at 430.447 kb on + strand, within MIT1002_00401 at 430.508 kb on - strand, within MIT1002_00401 at 430.543 kb on - strand, within MIT1002_00401 at 430.553 kb on - strand, within MIT1002_00401 at 430.618 kb on + strand, within MIT1002_00401 at 430.653 kb on + strand, within MIT1002_00401 at 430.653 kb on + strand, within MIT1002_00401 at 430.653 kb on + strand, within MIT1002_00401 at 430.661 kb on - strand, within MIT1002_00401 at 430.665 kb on + strand, within MIT1002_00401 at 430.673 kb on + strand, within MIT1002_00401 at 430.673 kb on - strand, within MIT1002_00401 at 430.673 kb on - strand, within MIT1002_00401
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 426,836 + -1.5 426,844 - +1.2 426,895 + +0.9 426,948 + -0.0 426,981 + -0.2 427,002 + -0.1 427,043 + -0.2 427,065 - -0.9 427,069 + -1.1 427,092 + -0.8 427,228 - MIT1002_00398 0.39 -0.7 427,365 + -0.2 427,439 + MIT1002_00399 0.11 -0.8 427,475 + MIT1002_00399 0.22 +0.2 427,520 - MIT1002_00399 0.37 -0.1 427,633 + MIT1002_00399 0.73 +0.1 427,672 - MIT1002_00399 0.86 +0.1 427,673 - MIT1002_00399 0.86 -0.5 427,757 + -0.0 427,780 + -0.6 427,780 + -2.1 427,788 - -1.0 427,795 + -0.4 427,803 - -0.2 427,968 + MIT1002_00400 0.10 +1.4 427,976 - MIT1002_00400 0.11 -2.6 427,976 - MIT1002_00400 0.11 -1.4 428,090 - MIT1002_00400 0.16 +0.2 428,111 + MIT1002_00400 0.18 -0.4 428,119 + MIT1002_00400 0.18 -0.2 428,128 - MIT1002_00400 0.18 +1.9 428,149 + MIT1002_00400 0.20 -0.9 428,157 - MIT1002_00400 0.20 -0.5 428,162 + MIT1002_00400 0.20 +0.2 428,298 - MIT1002_00400 0.27 +1.2 428,300 - MIT1002_00400 0.27 -0.9 428,308 - MIT1002_00400 0.28 +0.2 428,347 - MIT1002_00400 0.30 +0.3 428,444 - MIT1002_00400 0.35 -0.2 428,448 - MIT1002_00400 0.35 -0.7 428,509 - MIT1002_00400 0.38 +1.2 428,515 - MIT1002_00400 0.39 -0.6 428,576 + MIT1002_00400 0.42 -1.6 428,708 + MIT1002_00400 0.49 +0.1 428,716 - MIT1002_00400 0.49 +1.3 428,757 + MIT1002_00400 0.51 -1.3 428,844 + MIT1002_00400 0.56 -0.9 428,859 + MIT1002_00400 0.57 -0.5 428,867 - MIT1002_00400 0.57 -0.6 428,872 - MIT1002_00400 0.57 -1.8 428,980 - MIT1002_00400 0.63 +0.8 428,989 - MIT1002_00400 0.64 +0.7 428,992 + MIT1002_00400 0.64 +0.3 428,992 + MIT1002_00400 0.64 -0.6 429,048 + MIT1002_00400 0.67 -0.6 429,048 + MIT1002_00400 0.67 -0.5 429,056 - MIT1002_00400 0.67 -2.8 429,056 - MIT1002_00400 0.67 -0.1 429,137 - MIT1002_00400 0.71 +0.4 429,205 + MIT1002_00400 0.75 -0.5 429,213 + MIT1002_00400 0.75 -0.3 429,221 - MIT1002_00400 0.76 +0.1 429,221 - MIT1002_00400 0.76 -0.6 429,243 - MIT1002_00400 0.77 -0.4 429,416 + MIT1002_00400 0.86 +0.6 429,424 - MIT1002_00400 0.86 -0.7 429,480 - MIT1002_00400 0.89 +0.1 429,489 + MIT1002_00400 0.90 -1.8 429,496 + +0.0 429,496 + -0.1 429,504 - +0.4 429,510 + +0.1 429,510 + +0.0 429,513 - -0.4 429,532 - +1.5 429,727 + -0.2 429,768 + +1.1 429,776 - -0.8 429,776 - +0.7 429,776 - -0.0 429,776 - -1.5 429,778 + -1.4 429,778 + -0.1 429,778 + -0.2 429,778 + -0.7 429,778 + +0.1 429,778 + +0.6 429,783 + -0.2 429,783 + +0.9 429,786 - -0.2 429,791 - +0.2 429,791 - -0.3 429,868 - -0.1 429,887 - -3.2 430,026 + -1.8 430,054 - +0.2 430,078 + -0.7 430,078 + -0.2 430,086 - -0.2 430,086 - +1.2 430,091 + +0.3 430,190 - -0.3 430,280 + +2.1 430,280 + +0.3 430,280 + +0.2 430,280 + +2.2 430,288 - +0.4 430,314 - MIT1002_00401 0.10 -1.4 430,316 + MIT1002_00401 0.10 -0.0 430,316 + MIT1002_00401 0.10 +1.1 430,333 - MIT1002_00401 0.11 +1.0 430,338 + MIT1002_00401 0.11 -0.1 430,338 + MIT1002_00401 0.11 -1.9 430,338 + MIT1002_00401 0.11 -0.5 430,343 + MIT1002_00401 0.11 +0.3 430,346 - MIT1002_00401 0.12 -0.8 430,346 - MIT1002_00401 0.12 +1.4 430,447 + MIT1002_00401 0.16 -0.9 430,508 - MIT1002_00401 0.19 -1.5 430,543 - MIT1002_00401 0.21 -0.4 430,553 - MIT1002_00401 0.21 -0.1 430,618 + MIT1002_00401 0.24 -0.2 430,653 + MIT1002_00401 0.26 -0.8 430,653 + MIT1002_00401 0.26 +0.4 430,653 + MIT1002_00401 0.26 -1.2 430,661 - MIT1002_00401 0.26 -1.8 430,665 + MIT1002_00401 0.26 -0.6 430,673 + MIT1002_00401 0.26 +0.1 430,673 - MIT1002_00401 0.26 -0.9 430,673 - MIT1002_00401 0.26 +1.6
Or see this region's nucleotide sequence