Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00170 and MIT1002_00171 are separated by 141 nucleotides MIT1002_00171 and MIT1002_00172 are separated by 27 nucleotides MIT1002_00172 and MIT1002_00173 overlap by 4 nucleotides MIT1002_00173 and MIT1002_00174 are separated by 22 nucleotides
MIT1002_00170: MIT1002_00170 - Inorganic pyrophosphatase, at 186,104 to 186,634
_00170
MIT1002_00171: MIT1002_00171 - hypothetical protein, at 186,776 to 187,261
_00171
MIT1002_00172: MIT1002_00172 - 4-hydroxy-tetrahydrodipicolinate synthase, at 187,289 to 188,236
_00172
MIT1002_00173: MIT1002_00173 - hypothetical protein, at 188,233 to 188,931
_00173
MIT1002_00174: MIT1002_00174 - acyl-CoA thioester hydrolase, YbgC/YbaW family, at 188,954 to 189,373
_00174
Position (kb) 187
188
189 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 186.603 kb on + strand at 186.643 kb on + strand at 186.651 kb on - strand at 186.651 kb on - strand at 186.651 kb on - strand at 186.670 kb on + strand at 186.670 kb on + strand at 186.764 kb on + strand at 186.769 kb on + strand at 186.772 kb on - strand at 186.782 kb on - strand at 186.859 kb on - strand, within MIT1002_00171 at 186.905 kb on - strand, within MIT1002_00171 at 186.905 kb on - strand, within MIT1002_00171 at 186.905 kb on - strand, within MIT1002_00171 at 186.916 kb on + strand, within MIT1002_00171 at 186.923 kb on + strand, within MIT1002_00171 at 187.022 kb on + strand, within MIT1002_00171 at 187.044 kb on + strand, within MIT1002_00171 at 187.059 kb on - strand, within MIT1002_00171 at 187.059 kb on - strand, within MIT1002_00171 at 187.059 kb on - strand, within MIT1002_00171 at 187.184 kb on + strand, within MIT1002_00171 at 187.262 kb on + strand at 187.263 kb on + strand at 187.416 kb on + strand, within MIT1002_00172 at 187.416 kb on + strand, within MIT1002_00172 at 187.416 kb on + strand, within MIT1002_00172 at 187.419 kb on - strand, within MIT1002_00172 at 187.419 kb on - strand, within MIT1002_00172 at 187.423 kb on + strand, within MIT1002_00172 at 187.423 kb on + strand, within MIT1002_00172 at 187.423 kb on + strand, within MIT1002_00172 at 187.423 kb on + strand, within MIT1002_00172 at 187.423 kb on + strand, within MIT1002_00172 at 187.424 kb on - strand, within MIT1002_00172 at 187.424 kb on - strand, within MIT1002_00172 at 187.431 kb on - strand, within MIT1002_00172 at 187.431 kb on - strand, within MIT1002_00172 at 187.431 kb on - strand, within MIT1002_00172 at 187.431 kb on - strand, within MIT1002_00172 at 187.509 kb on + strand, within MIT1002_00172 at 187.535 kb on - strand, within MIT1002_00172 at 187.583 kb on - strand, within MIT1002_00172 at 187.585 kb on - strand, within MIT1002_00172 at 187.609 kb on + strand, within MIT1002_00172 at 187.664 kb on + strand, within MIT1002_00172 at 187.726 kb on - strand, within MIT1002_00172 at 187.792 kb on - strand, within MIT1002_00172 at 187.799 kb on - strand, within MIT1002_00172 at 187.827 kb on + strand, within MIT1002_00172 at 187.902 kb on - strand, within MIT1002_00172 at 187.906 kb on - strand, within MIT1002_00172 at 187.939 kb on - strand, within MIT1002_00172 at 187.950 kb on - strand, within MIT1002_00172 at 187.967 kb on + strand, within MIT1002_00172 at 187.975 kb on - strand, within MIT1002_00172 at 187.975 kb on - strand, within MIT1002_00172 at 187.992 kb on + strand, within MIT1002_00172 at 188.000 kb on - strand, within MIT1002_00172 at 188.020 kb on + strand, within MIT1002_00172 at 188.031 kb on - strand, within MIT1002_00172 at 188.110 kb on + strand, within MIT1002_00172 at 188.147 kb on - strand at 188.167 kb on + strand at 188.167 kb on + strand at 188.175 kb on - strand at 188.231 kb on + strand at 188.239 kb on - strand at 188.243 kb on + strand at 188.251 kb on - strand at 188.251 kb on - strand at 188.261 kb on - strand at 188.366 kb on + strand, within MIT1002_00173 at 188.366 kb on + strand, within MIT1002_00173 at 188.366 kb on + strand, within MIT1002_00173 at 188.374 kb on - strand, within MIT1002_00173 at 188.427 kb on + strand, within MIT1002_00173 at 188.466 kb on - strand, within MIT1002_00173 at 188.519 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.531 kb on + strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.539 kb on - strand, within MIT1002_00173 at 188.644 kb on + strand, within MIT1002_00173 at 188.690 kb on + strand, within MIT1002_00173 at 188.698 kb on - strand, within MIT1002_00173 at 188.713 kb on - strand, within MIT1002_00173 at 188.732 kb on - strand, within MIT1002_00173 at 188.844 kb on + strand, within MIT1002_00173 at 188.886 kb on + strand at 188.886 kb on + strand at 188.886 kb on + strand at 188.886 kb on + strand at 188.886 kb on + strand at 188.891 kb on + strand at 188.891 kb on + strand at 188.894 kb on - strand at 188.899 kb on - strand at 188.936 kb on + strand at 188.937 kb on - strand at 188.959 kb on - strand at 188.978 kb on - strand at 188.992 kb on - strand at 189.002 kb on - strand, within MIT1002_00174 at 189.005 kb on + strand, within MIT1002_00174 at 189.013 kb on - strand, within MIT1002_00174 at 189.016 kb on + strand, within MIT1002_00174 at 189.028 kb on + strand, within MIT1002_00174 at 189.049 kb on + strand, within MIT1002_00174 at 189.054 kb on + strand, within MIT1002_00174 at 189.069 kb on - strand, within MIT1002_00174 at 189.069 kb on - strand, within MIT1002_00174 at 189.227 kb on + strand, within MIT1002_00174 at 189.235 kb on - strand, within MIT1002_00174
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 186,603 + -0.0 186,643 + -0.7 186,651 - +0.1 186,651 - +0.8 186,651 - +0.1 186,670 + +0.1 186,670 + -0.5 186,764 + -0.0 186,769 + +0.1 186,772 - +0.6 186,782 - +2.4 186,859 - MIT1002_00171 0.17 -0.9 186,905 - MIT1002_00171 0.27 +0.1 186,905 - MIT1002_00171 0.27 +0.1 186,905 - MIT1002_00171 0.27 -4.1 186,916 + MIT1002_00171 0.29 -0.3 186,923 + MIT1002_00171 0.30 -0.4 187,022 + MIT1002_00171 0.51 -1.0 187,044 + MIT1002_00171 0.55 -1.0 187,059 - MIT1002_00171 0.58 +1.2 187,059 - MIT1002_00171 0.58 -1.7 187,059 - MIT1002_00171 0.58 -1.9 187,184 + MIT1002_00171 0.84 -0.3 187,262 + +1.0 187,263 + -0.3 187,416 + MIT1002_00172 0.13 -0.5 187,416 + MIT1002_00172 0.13 +1.9 187,416 + MIT1002_00172 0.13 +0.0 187,419 - MIT1002_00172 0.14 +0.3 187,419 - MIT1002_00172 0.14 -0.1 187,423 + MIT1002_00172 0.14 +0.9 187,423 + MIT1002_00172 0.14 -1.5 187,423 + MIT1002_00172 0.14 -0.5 187,423 + MIT1002_00172 0.14 -0.1 187,423 + MIT1002_00172 0.14 -0.7 187,424 - MIT1002_00172 0.14 +0.7 187,424 - MIT1002_00172 0.14 +0.2 187,431 - MIT1002_00172 0.15 -0.4 187,431 - MIT1002_00172 0.15 -1.4 187,431 - MIT1002_00172 0.15 -2.7 187,431 - MIT1002_00172 0.15 -0.8 187,509 + MIT1002_00172 0.23 +1.0 187,535 - MIT1002_00172 0.26 +1.4 187,583 - MIT1002_00172 0.31 +0.6 187,585 - MIT1002_00172 0.31 +1.9 187,609 + MIT1002_00172 0.34 +0.7 187,664 + MIT1002_00172 0.40 +0.1 187,726 - MIT1002_00172 0.46 -0.2 187,792 - MIT1002_00172 0.53 +0.2 187,799 - MIT1002_00172 0.54 +0.6 187,827 + MIT1002_00172 0.57 +0.6 187,902 - MIT1002_00172 0.65 -0.7 187,906 - MIT1002_00172 0.65 +0.1 187,939 - MIT1002_00172 0.69 +0.6 187,950 - MIT1002_00172 0.70 +0.1 187,967 + MIT1002_00172 0.72 -2.1 187,975 - MIT1002_00172 0.72 -0.3 187,975 - MIT1002_00172 0.72 +0.6 187,992 + MIT1002_00172 0.74 +0.8 188,000 - MIT1002_00172 0.75 -0.0 188,020 + MIT1002_00172 0.77 -0.1 188,031 - MIT1002_00172 0.78 -0.6 188,110 + MIT1002_00172 0.87 -0.2 188,147 - -2.7 188,167 + +0.2 188,167 + +1.1 188,175 - +0.2 188,231 + -0.8 188,239 - -0.6 188,243 + -0.4 188,251 - -0.9 188,251 - +1.1 188,261 - -0.4 188,366 + MIT1002_00173 0.19 -0.6 188,366 + MIT1002_00173 0.19 -0.3 188,366 + MIT1002_00173 0.19 -1.5 188,374 - MIT1002_00173 0.20 +1.7 188,427 + MIT1002_00173 0.28 -0.7 188,466 - MIT1002_00173 0.33 -0.2 188,519 + MIT1002_00173 0.41 -0.5 188,531 + MIT1002_00173 0.43 -1.5 188,531 + MIT1002_00173 0.43 -0.8 188,531 + MIT1002_00173 0.43 +0.1 188,531 + MIT1002_00173 0.43 +1.5 188,531 + MIT1002_00173 0.43 +1.6 188,531 + MIT1002_00173 0.43 -0.4 188,531 + MIT1002_00173 0.43 +0.6 188,531 + MIT1002_00173 0.43 +0.2 188,531 + MIT1002_00173 0.43 -0.2 188,531 + MIT1002_00173 0.43 +0.5 188,531 + MIT1002_00173 0.43 +0.8 188,531 + MIT1002_00173 0.43 +1.0 188,531 + MIT1002_00173 0.43 +1.4 188,531 + MIT1002_00173 0.43 -1.7 188,531 + MIT1002_00173 0.43 +0.3 188,531 + MIT1002_00173 0.43 -0.3 188,539 - MIT1002_00173 0.44 -0.6 188,539 - MIT1002_00173 0.44 +0.1 188,539 - MIT1002_00173 0.44 -0.6 188,539 - MIT1002_00173 0.44 +0.4 188,539 - MIT1002_00173 0.44 -0.1 188,539 - MIT1002_00173 0.44 +1.1 188,539 - MIT1002_00173 0.44 +1.2 188,539 - MIT1002_00173 0.44 +1.7 188,644 + MIT1002_00173 0.59 +0.8 188,690 + MIT1002_00173 0.65 +0.5 188,698 - MIT1002_00173 0.67 -0.2 188,713 - MIT1002_00173 0.69 +1.4 188,732 - MIT1002_00173 0.71 +0.8 188,844 + MIT1002_00173 0.87 +0.4 188,886 + -0.6 188,886 + +1.4 188,886 + -0.3 188,886 + +0.5 188,886 + +0.2 188,891 + +0.0 188,891 + +0.1 188,894 - +0.3 188,899 - +0.9 188,936 + +1.2 188,937 - +0.0 188,959 - -0.3 188,978 - +0.6 188,992 - -1.7 189,002 - MIT1002_00174 0.11 -0.5 189,005 + MIT1002_00174 0.12 +0.6 189,013 - MIT1002_00174 0.14 -1.8 189,016 + MIT1002_00174 0.15 +1.1 189,028 + MIT1002_00174 0.18 +0.2 189,049 + MIT1002_00174 0.23 +0.6 189,054 + MIT1002_00174 0.24 -0.4 189,069 - MIT1002_00174 0.27 +1.7 189,069 - MIT1002_00174 0.27 -0.1 189,227 + MIT1002_00174 0.65 -1.1 189,235 - MIT1002_00174 0.67 +1.2
Or see this region's nucleotide sequence