Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS29960 and QEN71_RS29965 are separated by 49 nucleotides QEN71_RS29965 and QEN71_RS29970 overlap by 4 nucleotides QEN71_RS29970 and QEN71_RS29975 are separated by 114 nucleotides
QEN71_RS29960: QEN71_RS29960 - sugar phosphate isomerase/epimerase, at 67,617 to 68,450
_RS29960
QEN71_RS29965: QEN71_RS29965 - NIPSNAP family protein, at 68,500 to 69,111
_RS29965
QEN71_RS29970: QEN71_RS29970 - FAD-dependent oxidoreductase, at 69,108 to 70,859
_RS29970
QEN71_RS29975: QEN71_RS29975 - NAD(P)-dependent oxidoreductase, at 70,974 to 71,879
_RS29975
Position (kb) 69
70
71 Strain fitness (log2 ratio) -2
-1
0
1 at 68.140 kb on + strand, within QEN71_RS29960 at 68.463 kb on - strand at 68.531 kb on + strand at 68.531 kb on + strand at 68.531 kb on + strand at 68.531 kb on + strand at 68.531 kb on + strand at 68.532 kb on - strand at 68.532 kb on - strand at 68.532 kb on - strand at 68.532 kb on - strand at 68.684 kb on + strand, within QEN71_RS29965 at 68.684 kb on + strand, within QEN71_RS29965 at 68.684 kb on + strand, within QEN71_RS29965 at 68.751 kb on + strand, within QEN71_RS29965 at 68.751 kb on + strand, within QEN71_RS29965 at 68.751 kb on + strand, within QEN71_RS29965 at 68.751 kb on + strand, within QEN71_RS29965 at 68.752 kb on - strand, within QEN71_RS29965 at 68.752 kb on - strand, within QEN71_RS29965 at 68.780 kb on + strand, within QEN71_RS29965 at 68.780 kb on + strand, within QEN71_RS29965 at 68.795 kb on + strand, within QEN71_RS29965 at 68.796 kb on - strand, within QEN71_RS29965 at 68.796 kb on - strand, within QEN71_RS29965 at 68.997 kb on - strand, within QEN71_RS29965 at 69.101 kb on + strand at 69.101 kb on + strand at 69.101 kb on + strand at 69.101 kb on + strand at 69.101 kb on + strand at 69.101 kb on + strand at 69.102 kb on - strand at 69.102 kb on - strand at 69.193 kb on + strand at 69.280 kb on + strand at 69.280 kb on + strand at 69.281 kb on - strand at 69.385 kb on + strand, within QEN71_RS29970 at 69.386 kb on - strand, within QEN71_RS29970 at 69.457 kb on + strand, within QEN71_RS29970 at 69.506 kb on - strand, within QEN71_RS29970 at 69.613 kb on + strand, within QEN71_RS29970 at 69.614 kb on - strand, within QEN71_RS29970 at 69.752 kb on - strand, within QEN71_RS29970 at 70.004 kb on + strand, within QEN71_RS29970 at 70.004 kb on + strand, within QEN71_RS29970 at 70.300 kb on + strand, within QEN71_RS29970 at 70.301 kb on - strand, within QEN71_RS29970 at 70.301 kb on - strand, within QEN71_RS29970 at 70.521 kb on - strand, within QEN71_RS29970 at 71.320 kb on - strand, within QEN71_RS29975 at 71.320 kb on - strand, within QEN71_RS29975 at 71.320 kb on - strand, within QEN71_RS29975 at 71.382 kb on + strand, within QEN71_RS29975 at 71.383 kb on - strand, within QEN71_RS29975 at 71.424 kb on + strand, within QEN71_RS29975 at 71.551 kb on - strand, within QEN71_RS29975 at 71.611 kb on - strand, within QEN71_RS29975
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 68,140 + QEN71_RS29960 0.63 +0.2 68,463 - -0.3 68,531 + -0.6 68,531 + +0.7 68,531 + -0.4 68,531 + +1.3 68,531 + -0.5 68,532 - +0.2 68,532 - +0.4 68,532 - -0.4 68,532 - -0.2 68,684 + QEN71_RS29965 0.30 -0.4 68,684 + QEN71_RS29965 0.30 -2.0 68,684 + QEN71_RS29965 0.30 +1.1 68,751 + QEN71_RS29965 0.41 -0.9 68,751 + QEN71_RS29965 0.41 +0.3 68,751 + QEN71_RS29965 0.41 +0.7 68,751 + QEN71_RS29965 0.41 -0.6 68,752 - QEN71_RS29965 0.41 +0.7 68,752 - QEN71_RS29965 0.41 +0.6 68,780 + QEN71_RS29965 0.46 -0.3 68,780 + QEN71_RS29965 0.46 -0.3 68,795 + QEN71_RS29965 0.48 +0.0 68,796 - QEN71_RS29965 0.48 +1.1 68,796 - QEN71_RS29965 0.48 -0.7 68,997 - QEN71_RS29965 0.81 +1.5 69,101 + -0.0 69,101 + +0.3 69,101 + +0.1 69,101 + +0.1 69,101 + -1.0 69,101 + -0.7 69,102 - -0.9 69,102 - +0.9 69,193 + +0.4 69,280 + -0.4 69,280 + +0.6 69,281 - +0.3 69,385 + QEN71_RS29970 0.16 +0.3 69,386 - QEN71_RS29970 0.16 +1.3 69,457 + QEN71_RS29970 0.20 -1.0 69,506 - QEN71_RS29970 0.23 +0.1 69,613 + QEN71_RS29970 0.29 -0.1 69,614 - QEN71_RS29970 0.29 -0.4 69,752 - QEN71_RS29970 0.37 +0.6 70,004 + QEN71_RS29970 0.51 -0.7 70,004 + QEN71_RS29970 0.51 -0.4 70,300 + QEN71_RS29970 0.68 -1.7 70,301 - QEN71_RS29970 0.68 +0.1 70,301 - QEN71_RS29970 0.68 -0.1 70,521 - QEN71_RS29970 0.81 +0.7 71,320 - QEN71_RS29975 0.38 -0.4 71,320 - QEN71_RS29975 0.38 +1.6 71,320 - QEN71_RS29975 0.38 +0.4 71,382 + QEN71_RS29975 0.45 -1.3 71,383 - QEN71_RS29975 0.45 -1.9 71,424 + QEN71_RS29975 0.50 -2.2 71,551 - QEN71_RS29975 0.64 +0.8 71,611 - QEN71_RS29975 0.70 -1.7
Or see this region's nucleotide sequence