Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_04144 and MIT1002_04145 are separated by 298 nucleotides MIT1002_04145 and MIT1002_04146 are separated by 175 nucleotides MIT1002_04146 and MIT1002_04147 are separated by 7 nucleotides MIT1002_04147 and MIT1002_04148 are separated by 84 nucleotides
MIT1002_04144: MIT1002_04144 - hypothetical protein, at 9,664 to 9,993
_04144
MIT1002_04145: MIT1002_04145 - hypothetical protein, at 10,292 to 10,396
_04145
MIT1002_04146: MIT1002_04146 - Putative transposon Tn552 DNA-invertase bin3, at 10,572 to 11,207
_04146
MIT1002_04147: MIT1002_04147 - hypothetical protein, at 11,215 to 11,580
_04147
MIT1002_04148: MIT1002_04148 - Transposase, TnpA family, at 11,665 to 14,403
_04148
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 9.627 kb on - strand at 9.642 kb on - strand at 9.689 kb on + strand at 9.729 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.790 kb on + strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.798 kb on - strand, within MIT1002_04144 at 9.808 kb on - strand, within MIT1002_04144 at 9.883 kb on - strand, within MIT1002_04144 at 9.911 kb on - strand, within MIT1002_04144 at 9.957 kb on + strand, within MIT1002_04144 at 9.967 kb on + strand at 10.076 kb on + strand at 10.084 kb on - strand at 10.247 kb on - strand at 10.266 kb on - strand at 10.281 kb on + strand at 10.308 kb on + strand, within MIT1002_04145 at 10.376 kb on - strand, within MIT1002_04145 at 10.385 kb on + strand, within MIT1002_04145 at 10.388 kb on - strand at 10.388 kb on - strand at 10.476 kb on + strand at 10.538 kb on + strand at 10.538 kb on + strand at 10.538 kb on + strand at 10.546 kb on - strand at 10.546 kb on - strand at 10.546 kb on - strand at 10.546 kb on - strand at 10.581 kb on + strand at 10.589 kb on + strand at 10.605 kb on + strand at 10.659 kb on + strand, within MIT1002_04146 at 10.667 kb on - strand, within MIT1002_04146 at 10.762 kb on - strand, within MIT1002_04146 at 10.789 kb on + strand, within MIT1002_04146 at 10.797 kb on - strand, within MIT1002_04146 at 10.797 kb on - strand, within MIT1002_04146 at 10.797 kb on - strand, within MIT1002_04146 at 10.797 kb on - strand, within MIT1002_04146 at 10.797 kb on - strand, within MIT1002_04146 at 10.882 kb on + strand, within MIT1002_04146 at 11.015 kb on - strand, within MIT1002_04146 at 11.042 kb on + strand, within MIT1002_04146 at 11.042 kb on + strand, within MIT1002_04146 at 11.042 kb on + strand, within MIT1002_04146 at 11.129 kb on + strand, within MIT1002_04146 at 11.140 kb on + strand, within MIT1002_04146 at 11.154 kb on + strand at 11.242 kb on - strand at 11.264 kb on - strand, within MIT1002_04147 at 11.277 kb on - strand, within MIT1002_04147 at 11.277 kb on - strand, within MIT1002_04147 at 11.386 kb on + strand, within MIT1002_04147 at 11.497 kb on + strand, within MIT1002_04147 at 11.529 kb on + strand, within MIT1002_04147 at 11.582 kb on - strand at 11.676 kb on + strand at 11.736 kb on - strand at 11.783 kb on - strand at 11.790 kb on - strand at 11.813 kb on + strand at 11.836 kb on + strand at 11.849 kb on - strand at 11.952 kb on + strand, within MIT1002_04148 at 11.971 kb on + strand, within MIT1002_04148 at 11.979 kb on - strand, within MIT1002_04148 at 11.988 kb on + strand, within MIT1002_04148 at 11.988 kb on + strand, within MIT1002_04148 at 11.997 kb on - strand, within MIT1002_04148 at 12.053 kb on + strand, within MIT1002_04148 at 12.073 kb on - strand, within MIT1002_04148
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 9,627 - -0.3 9,642 - -1.1 9,689 + -0.4 9,729 + MIT1002_04144 0.20 -0.2 9,790 + MIT1002_04144 0.38 -0.1 9,790 + MIT1002_04144 0.38 -0.1 9,790 + MIT1002_04144 0.38 -0.8 9,790 + MIT1002_04144 0.38 +0.6 9,790 + MIT1002_04144 0.38 -0.1 9,790 + MIT1002_04144 0.38 -0.4 9,790 + MIT1002_04144 0.38 -0.4 9,790 + MIT1002_04144 0.38 -2.2 9,790 + MIT1002_04144 0.38 -0.1 9,790 + MIT1002_04144 0.38 +1.5 9,790 + MIT1002_04144 0.38 +0.1 9,790 + MIT1002_04144 0.38 -1.4 9,790 + MIT1002_04144 0.38 -0.3 9,790 + MIT1002_04144 0.38 -0.6 9,790 + MIT1002_04144 0.38 +0.2 9,790 + MIT1002_04144 0.38 +0.8 9,790 + MIT1002_04144 0.38 +1.0 9,790 + MIT1002_04144 0.38 +0.6 9,790 + MIT1002_04144 0.38 +0.2 9,790 + MIT1002_04144 0.38 -1.0 9,790 + MIT1002_04144 0.38 +0.8 9,790 + MIT1002_04144 0.38 -1.1 9,790 + MIT1002_04144 0.38 +0.2 9,790 + MIT1002_04144 0.38 +0.9 9,790 + MIT1002_04144 0.38 -0.4 9,798 - MIT1002_04144 0.41 +0.1 9,798 - MIT1002_04144 0.41 -0.4 9,798 - MIT1002_04144 0.41 +0.4 9,798 - MIT1002_04144 0.41 -0.6 9,798 - MIT1002_04144 0.41 -1.8 9,798 - MIT1002_04144 0.41 +0.5 9,798 - MIT1002_04144 0.41 +0.5 9,798 - MIT1002_04144 0.41 -1.3 9,798 - MIT1002_04144 0.41 +0.5 9,798 - MIT1002_04144 0.41 -0.3 9,798 - MIT1002_04144 0.41 -0.4 9,798 - MIT1002_04144 0.41 +1.4 9,798 - MIT1002_04144 0.41 -0.4 9,798 - MIT1002_04144 0.41 +0.4 9,798 - MIT1002_04144 0.41 -0.8 9,798 - MIT1002_04144 0.41 +0.4 9,798 - MIT1002_04144 0.41 -0.6 9,798 - MIT1002_04144 0.41 -0.1 9,798 - MIT1002_04144 0.41 -2.2 9,808 - MIT1002_04144 0.44 +0.0 9,883 - MIT1002_04144 0.66 +0.4 9,911 - MIT1002_04144 0.75 +0.8 9,957 + MIT1002_04144 0.89 +0.2 9,967 + +0.4 10,076 + -0.1 10,084 - -0.7 10,247 - +2.3 10,266 - -0.4 10,281 + +0.5 10,308 + MIT1002_04145 0.15 +0.0 10,376 - MIT1002_04145 0.80 -2.0 10,385 + MIT1002_04145 0.89 -1.7 10,388 - +0.8 10,388 - +0.4 10,476 + +2.3 10,538 + +0.0 10,538 + -2.2 10,538 + +0.1 10,546 - -0.8 10,546 - +0.3 10,546 - +2.5 10,546 - +0.1 10,581 + -1.3 10,589 + +0.8 10,605 + -1.1 10,659 + MIT1002_04146 0.14 -1.4 10,667 - MIT1002_04146 0.15 -0.1 10,762 - MIT1002_04146 0.30 -1.1 10,789 + MIT1002_04146 0.34 -2.1 10,797 - MIT1002_04146 0.35 +1.1 10,797 - MIT1002_04146 0.35 -1.5 10,797 - MIT1002_04146 0.35 +0.2 10,797 - MIT1002_04146 0.35 +1.7 10,797 - MIT1002_04146 0.35 +1.4 10,882 + MIT1002_04146 0.49 -1.4 11,015 - MIT1002_04146 0.70 +0.1 11,042 + MIT1002_04146 0.74 -0.8 11,042 + MIT1002_04146 0.74 -0.7 11,042 + MIT1002_04146 0.74 -1.0 11,129 + MIT1002_04146 0.88 +0.3 11,140 + MIT1002_04146 0.89 -0.0 11,154 + -0.2 11,242 - -0.6 11,264 - MIT1002_04147 0.13 +0.7 11,277 - MIT1002_04147 0.17 +0.2 11,277 - MIT1002_04147 0.17 -0.6 11,386 + MIT1002_04147 0.47 +0.4 11,497 + MIT1002_04147 0.77 +0.3 11,529 + MIT1002_04147 0.86 -1.4 11,582 - -2.5 11,676 + +0.1 11,736 - +0.0 11,783 - -0.5 11,790 - -0.5 11,813 + +0.8 11,836 + +0.6 11,849 - -0.5 11,952 + MIT1002_04148 0.10 +0.5 11,971 + MIT1002_04148 0.11 -0.7 11,979 - MIT1002_04148 0.11 -0.5 11,988 + MIT1002_04148 0.12 -1.3 11,988 + MIT1002_04148 0.12 -0.6 11,997 - MIT1002_04148 0.12 -0.4 12,053 + MIT1002_04148 0.14 +1.1 12,073 - MIT1002_04148 0.15 +0.2
Or see this region's nucleotide sequence