Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03116 and MIT1002_03117 are separated by 178 nucleotides MIT1002_03117 and MIT1002_03118 are separated by 23 nucleotides MIT1002_03118 and MIT1002_03119 are separated by 40 nucleotides
MIT1002_03116: MIT1002_03116 - Spore coat protein SA, at 3,470,624 to 3,471,712
_03116
MIT1002_03117: MIT1002_03117 - DnaA initiator-associating protein DiaA, at 3,471,891 to 3,472,487
_03117
MIT1002_03118: MIT1002_03118 - Osmotically-inducible protein Y precursor, at 3,472,511 to 3,473,086
_03118
MIT1002_03119: MIT1002_03119 - High-copy suppressor of rspA, at 3,473,127 to 3,474,488
_03119
Position (kb)
3471
3472
3473 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3470.897 kb on - strand, within MIT1002_03116 at 3470.941 kb on + strand, within MIT1002_03116 at 3470.959 kb on - strand, within MIT1002_03116 at 3471.004 kb on - strand, within MIT1002_03116 at 3471.183 kb on + strand, within MIT1002_03116 at 3471.183 kb on + strand, within MIT1002_03116 at 3471.193 kb on + strand, within MIT1002_03116 at 3471.266 kb on - strand, within MIT1002_03116 at 3471.345 kb on + strand, within MIT1002_03116 at 3471.353 kb on - strand, within MIT1002_03116 at 3471.427 kb on - strand, within MIT1002_03116 at 3471.438 kb on + strand, within MIT1002_03116 at 3471.438 kb on + strand, within MIT1002_03116 at 3471.438 kb on + strand, within MIT1002_03116 at 3471.439 kb on - strand, within MIT1002_03116 at 3471.476 kb on - strand, within MIT1002_03116 at 3471.504 kb on + strand, within MIT1002_03116 at 3471.525 kb on - strand, within MIT1002_03116 at 3471.584 kb on + strand, within MIT1002_03116 at 3471.617 kb on + strand at 3471.622 kb on + strand at 3471.704 kb on + strand at 3471.710 kb on - strand at 3471.778 kb on + strand at 3471.778 kb on + strand at 3471.786 kb on - strand at 3471.786 kb on - strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.826 kb on + strand at 3471.834 kb on - strand at 3471.834 kb on - strand at 3471.834 kb on - strand at 3471.900 kb on - strand at 3471.972 kb on - strand, within MIT1002_03117 at 3471.991 kb on + strand, within MIT1002_03117 at 3471.999 kb on + strand, within MIT1002_03117 at 3472.020 kb on + strand, within MIT1002_03117 at 3472.138 kb on + strand, within MIT1002_03117 at 3472.143 kb on + strand, within MIT1002_03117 at 3472.143 kb on + strand, within MIT1002_03117 at 3472.146 kb on - strand, within MIT1002_03117 at 3472.229 kb on - strand, within MIT1002_03117 at 3472.262 kb on + strand, within MIT1002_03117 at 3472.270 kb on - strand, within MIT1002_03117 at 3472.566 kb on + strand at 3472.566 kb on + strand at 3472.566 kb on + strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.567 kb on - strand at 3472.623 kb on + strand, within MIT1002_03118 at 3472.631 kb on - strand, within MIT1002_03118 at 3472.724 kb on - strand, within MIT1002_03118 at 3472.730 kb on + strand, within MIT1002_03118 at 3472.740 kb on + strand, within MIT1002_03118 at 3472.748 kb on - strand, within MIT1002_03118 at 3472.753 kb on - strand, within MIT1002_03118 at 3472.775 kb on + strand, within MIT1002_03118 at 3472.790 kb on + strand, within MIT1002_03118 at 3472.811 kb on - strand, within MIT1002_03118 at 3472.863 kb on + strand, within MIT1002_03118 at 3472.863 kb on + strand, within MIT1002_03118 at 3472.893 kb on - strand, within MIT1002_03118 at 3472.948 kb on + strand, within MIT1002_03118 at 3472.948 kb on + strand, within MIT1002_03118 at 3472.948 kb on + strand, within MIT1002_03118 at 3473.068 kb on - strand at 3473.088 kb on - strand at 3473.098 kb on - strand at 3473.098 kb on - strand at 3473.197 kb on + strand at 3473.334 kb on + strand, within MIT1002_03119 at 3473.350 kb on + strand, within MIT1002_03119 at 3473.399 kb on - strand, within MIT1002_03119 at 3473.399 kb on - strand, within MIT1002_03119 at 3473.433 kb on - strand, within MIT1002_03119 at 3473.483 kb on + strand, within MIT1002_03119
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 3,470,897 - MIT1002_03116 0.25 -0.6 3,470,941 + MIT1002_03116 0.29 +1.8 3,470,959 - MIT1002_03116 0.31 +1.7 3,471,004 - MIT1002_03116 0.35 +0.8 3,471,183 + MIT1002_03116 0.51 +2.5 3,471,183 + MIT1002_03116 0.51 +1.8 3,471,193 + MIT1002_03116 0.52 +3.2 3,471,266 - MIT1002_03116 0.59 +1.6 3,471,345 + MIT1002_03116 0.66 +1.9 3,471,353 - MIT1002_03116 0.67 +0.0 3,471,427 - MIT1002_03116 0.74 +1.3 3,471,438 + MIT1002_03116 0.75 +2.0 3,471,438 + MIT1002_03116 0.75 +1.5 3,471,438 + MIT1002_03116 0.75 +2.8 3,471,439 - MIT1002_03116 0.75 +1.8 3,471,476 - MIT1002_03116 0.78 -0.7 3,471,504 + MIT1002_03116 0.81 -0.2 3,471,525 - MIT1002_03116 0.83 +0.7 3,471,584 + MIT1002_03116 0.88 +2.7 3,471,617 + +2.5 3,471,622 + +1.6 3,471,704 + +0.2 3,471,710 - +0.3 3,471,778 + -1.4 3,471,778 + +0.9 3,471,786 - -2.6 3,471,786 - -0.4 3,471,826 + -1.5 3,471,826 + +0.5 3,471,826 + +0.0 3,471,826 + +2.9 3,471,826 + -1.7 3,471,834 - +0.3 3,471,834 - -0.4 3,471,834 - -1.2 3,471,900 - -0.5 3,471,972 - MIT1002_03117 0.14 -0.8 3,471,991 + MIT1002_03117 0.17 -1.0 3,471,999 + MIT1002_03117 0.18 +0.8 3,472,020 + MIT1002_03117 0.22 +0.8 3,472,138 + MIT1002_03117 0.41 -2.2 3,472,143 + MIT1002_03117 0.42 -0.5 3,472,143 + MIT1002_03117 0.42 +0.0 3,472,146 - MIT1002_03117 0.43 -2.2 3,472,229 - MIT1002_03117 0.57 -1.2 3,472,262 + MIT1002_03117 0.62 -1.1 3,472,270 - MIT1002_03117 0.63 -1.1 3,472,566 + +0.8 3,472,566 + +0.5 3,472,566 + +0.0 3,472,567 - -0.4 3,472,567 - -0.3 3,472,567 - +0.5 3,472,567 - -0.3 3,472,567 - +0.0 3,472,623 + MIT1002_03118 0.19 +1.1 3,472,631 - MIT1002_03118 0.21 -0.9 3,472,724 - MIT1002_03118 0.37 -1.5 3,472,730 + MIT1002_03118 0.38 -1.7 3,472,740 + MIT1002_03118 0.40 -0.7 3,472,748 - MIT1002_03118 0.41 -0.7 3,472,753 - MIT1002_03118 0.42 -0.2 3,472,775 + MIT1002_03118 0.46 +1.2 3,472,790 + MIT1002_03118 0.48 -1.6 3,472,811 - MIT1002_03118 0.52 -1.0 3,472,863 + MIT1002_03118 0.61 +0.6 3,472,863 + MIT1002_03118 0.61 +0.9 3,472,893 - MIT1002_03118 0.66 -0.1 3,472,948 + MIT1002_03118 0.76 -2.3 3,472,948 + MIT1002_03118 0.76 -0.1 3,472,948 + MIT1002_03118 0.76 -0.1 3,473,068 - +0.1 3,473,088 - -0.5 3,473,098 - -1.4 3,473,098 - -0.2 3,473,197 + +0.9 3,473,334 + MIT1002_03119 0.15 +0.3 3,473,350 + MIT1002_03119 0.16 +0.3 3,473,399 - MIT1002_03119 0.20 +2.2 3,473,399 - MIT1002_03119 0.20 -0.4 3,473,433 - MIT1002_03119 0.22 -1.6 3,473,483 + MIT1002_03119 0.26 +1.3
Or see this region's nucleotide sequence