Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02264 and MIT1002_02265 are separated by 239 nucleotides MIT1002_02265 and MIT1002_02266 are separated by 110 nucleotides
MIT1002_02264: MIT1002_02264 - FMN-dependent NADH-azoreductase, at 2,519,700 to 2,520,302
_02264
MIT1002_02265: MIT1002_02265 - CHRD domain protein, at 2,520,542 to 2,523,007
_02265
MIT1002_02266: MIT1002_02266 - Methionine gamma-lyase, at 2,523,118 to 2,524,359
_02266
Position (kb)
2520
2521
2522
2523
2524 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2519.547 kb on - strand at 2519.549 kb on + strand at 2519.557 kb on - strand at 2519.557 kb on - strand at 2519.557 kb on - strand at 2519.561 kb on + strand at 2519.597 kb on - strand at 2519.651 kb on - strand at 2519.698 kb on + strand at 2519.706 kb on - strand at 2519.859 kb on + strand, within MIT1002_02264 at 2519.859 kb on + strand, within MIT1002_02264 at 2519.859 kb on + strand, within MIT1002_02264 at 2519.867 kb on - strand, within MIT1002_02264 at 2519.874 kb on - strand, within MIT1002_02264 at 2519.930 kb on + strand, within MIT1002_02264 at 2519.965 kb on + strand, within MIT1002_02264 at 2520.164 kb on + strand, within MIT1002_02264 at 2520.164 kb on + strand, within MIT1002_02264 at 2520.172 kb on - strand, within MIT1002_02264 at 2520.172 kb on - strand, within MIT1002_02264 at 2520.172 kb on - strand, within MIT1002_02264 at 2520.172 kb on - strand, within MIT1002_02264 at 2520.172 kb on - strand, within MIT1002_02264 at 2520.182 kb on - strand, within MIT1002_02264 at 2520.187 kb on + strand, within MIT1002_02264 at 2520.465 kb on + strand at 2520.499 kb on + strand at 2520.507 kb on - strand at 2520.507 kb on - strand at 2520.512 kb on - strand at 2520.639 kb on + strand at 2520.639 kb on - strand at 2520.666 kb on - strand at 2520.677 kb on + strand at 2520.677 kb on + strand at 2520.677 kb on + strand at 2520.677 kb on + strand at 2520.677 kb on + strand at 2520.708 kb on - strand at 2520.843 kb on - strand, within MIT1002_02265 at 2520.897 kb on - strand, within MIT1002_02265 at 2520.923 kb on + strand, within MIT1002_02265 at 2521.085 kb on + strand, within MIT1002_02265 at 2521.149 kb on - strand, within MIT1002_02265 at 2521.156 kb on - strand, within MIT1002_02265 at 2521.193 kb on - strand, within MIT1002_02265 at 2521.321 kb on - strand, within MIT1002_02265 at 2521.321 kb on - strand, within MIT1002_02265 at 2521.398 kb on + strand, within MIT1002_02265 at 2521.433 kb on + strand, within MIT1002_02265 at 2521.495 kb on - strand, within MIT1002_02265 at 2521.510 kb on + strand, within MIT1002_02265 at 2521.510 kb on + strand, within MIT1002_02265 at 2521.510 kb on + strand, within MIT1002_02265 at 2521.518 kb on - strand, within MIT1002_02265 at 2521.518 kb on - strand, within MIT1002_02265 at 2521.568 kb on + strand, within MIT1002_02265 at 2521.576 kb on - strand, within MIT1002_02265 at 2521.610 kb on + strand, within MIT1002_02265 at 2521.624 kb on - strand, within MIT1002_02265 at 2521.630 kb on + strand, within MIT1002_02265 at 2521.630 kb on + strand, within MIT1002_02265 at 2521.700 kb on + strand, within MIT1002_02265 at 2521.751 kb on + strand, within MIT1002_02265 at 2521.761 kb on + strand, within MIT1002_02265 at 2521.775 kb on - strand, within MIT1002_02265 at 2521.781 kb on - strand, within MIT1002_02265 at 2521.791 kb on - strand, within MIT1002_02265 at 2521.818 kb on + strand, within MIT1002_02265 at 2521.834 kb on + strand, within MIT1002_02265 at 2521.834 kb on + strand, within MIT1002_02265 at 2521.834 kb on + strand, within MIT1002_02265 at 2521.842 kb on - strand, within MIT1002_02265 at 2521.902 kb on + strand, within MIT1002_02265 at 2521.907 kb on + strand, within MIT1002_02265 at 2521.993 kb on + strand, within MIT1002_02265 at 2522.049 kb on + strand, within MIT1002_02265 at 2522.049 kb on + strand, within MIT1002_02265 at 2522.051 kb on - strand, within MIT1002_02265 at 2522.074 kb on + strand, within MIT1002_02265 at 2522.108 kb on + strand, within MIT1002_02265 at 2522.135 kb on - strand, within MIT1002_02265 at 2522.162 kb on - strand, within MIT1002_02265 at 2522.165 kb on - strand, within MIT1002_02265 at 2522.206 kb on + strand, within MIT1002_02265 at 2522.214 kb on - strand, within MIT1002_02265 at 2522.223 kb on + strand, within MIT1002_02265 at 2522.231 kb on + strand, within MIT1002_02265 at 2522.246 kb on - strand, within MIT1002_02265 at 2522.248 kb on + strand, within MIT1002_02265 at 2522.256 kb on - strand, within MIT1002_02265 at 2522.256 kb on - strand, within MIT1002_02265 at 2522.256 kb on - strand, within MIT1002_02265 at 2522.284 kb on - strand, within MIT1002_02265 at 2522.344 kb on - strand, within MIT1002_02265 at 2522.344 kb on - strand, within MIT1002_02265 at 2522.369 kb on + strand, within MIT1002_02265 at 2522.371 kb on + strand, within MIT1002_02265 at 2522.371 kb on + strand, within MIT1002_02265 at 2522.379 kb on - strand, within MIT1002_02265 at 2522.379 kb on - strand, within MIT1002_02265 at 2522.381 kb on + strand, within MIT1002_02265 at 2522.440 kb on + strand, within MIT1002_02265 at 2522.440 kb on + strand, within MIT1002_02265 at 2522.448 kb on - strand, within MIT1002_02265 at 2522.448 kb on - strand, within MIT1002_02265 at 2522.495 kb on + strand, within MIT1002_02265 at 2522.540 kb on - strand, within MIT1002_02265 at 2522.602 kb on + strand, within MIT1002_02265 at 2522.610 kb on - strand, within MIT1002_02265 at 2522.706 kb on - strand, within MIT1002_02265 at 2522.708 kb on - strand, within MIT1002_02265 at 2522.763 kb on + strand at 2522.818 kb on - strand at 2522.871 kb on + strand at 2522.881 kb on + strand at 2522.881 kb on + strand at 2522.889 kb on - strand at 2523.118 kb on + strand at 2523.147 kb on - strand at 2523.156 kb on + strand at 2523.164 kb on - strand at 2523.176 kb on - strand at 2523.176 kb on - strand at 2523.176 kb on - strand at 2523.176 kb on - strand at 2523.205 kb on - strand at 2523.225 kb on + strand at 2523.233 kb on - strand at 2523.365 kb on - strand, within MIT1002_02266 at 2523.495 kb on - strand, within MIT1002_02266 at 2523.502 kb on - strand, within MIT1002_02266 at 2523.530 kb on - strand, within MIT1002_02266 at 2523.627 kb on + strand, within MIT1002_02266 at 2523.653 kb on + strand, within MIT1002_02266 at 2523.653 kb on + strand, within MIT1002_02266 at 2523.661 kb on - strand, within MIT1002_02266 at 2523.679 kb on + strand, within MIT1002_02266 at 2523.689 kb on + strand, within MIT1002_02266 at 2523.818 kb on + strand, within MIT1002_02266 at 2523.818 kb on + strand, within MIT1002_02266 at 2523.826 kb on - strand, within MIT1002_02266 at 2523.887 kb on + strand, within MIT1002_02266 at 2523.899 kb on + strand, within MIT1002_02266 at 2523.963 kb on + strand, within MIT1002_02266 at 2523.963 kb on + strand, within MIT1002_02266 at 2523.963 kb on + strand, within MIT1002_02266 at 2523.963 kb on + strand, within MIT1002_02266 at 2523.966 kb on - strand, within MIT1002_02266 at 2523.971 kb on - strand, within MIT1002_02266
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 2,519,547 - -1.1 2,519,549 + +0.2 2,519,557 - +1.0 2,519,557 - +0.7 2,519,557 - +1.7 2,519,561 + +1.1 2,519,597 - -0.9 2,519,651 - -1.7 2,519,698 + +0.2 2,519,706 - +1.0 2,519,859 + MIT1002_02264 0.26 -0.8 2,519,859 + MIT1002_02264 0.26 -0.7 2,519,859 + MIT1002_02264 0.26 -0.8 2,519,867 - MIT1002_02264 0.28 -0.2 2,519,874 - MIT1002_02264 0.29 -0.2 2,519,930 + MIT1002_02264 0.38 -0.4 2,519,965 + MIT1002_02264 0.44 +1.3 2,520,164 + MIT1002_02264 0.77 -1.0 2,520,164 + MIT1002_02264 0.77 +0.4 2,520,172 - MIT1002_02264 0.78 +0.0 2,520,172 - MIT1002_02264 0.78 -0.5 2,520,172 - MIT1002_02264 0.78 -2.4 2,520,172 - MIT1002_02264 0.78 -0.3 2,520,172 - MIT1002_02264 0.78 -0.3 2,520,182 - MIT1002_02264 0.80 +3.3 2,520,187 + MIT1002_02264 0.81 -0.3 2,520,465 + -0.2 2,520,499 + -1.1 2,520,507 - -0.8 2,520,507 - -0.9 2,520,512 - -0.2 2,520,639 + -1.8 2,520,639 - -0.3 2,520,666 - +0.0 2,520,677 + +0.5 2,520,677 + +0.6 2,520,677 + -0.5 2,520,677 + +0.4 2,520,677 + +1.5 2,520,708 - +0.1 2,520,843 - MIT1002_02265 0.12 -1.0 2,520,897 - MIT1002_02265 0.14 +0.8 2,520,923 + MIT1002_02265 0.15 +1.8 2,521,085 + MIT1002_02265 0.22 -0.4 2,521,149 - MIT1002_02265 0.25 +0.5 2,521,156 - MIT1002_02265 0.25 -0.7 2,521,193 - MIT1002_02265 0.26 +1.0 2,521,321 - MIT1002_02265 0.32 -0.8 2,521,321 - MIT1002_02265 0.32 -0.2 2,521,398 + MIT1002_02265 0.35 -0.8 2,521,433 + MIT1002_02265 0.36 -1.2 2,521,495 - MIT1002_02265 0.39 +0.5 2,521,510 + MIT1002_02265 0.39 -0.2 2,521,510 + MIT1002_02265 0.39 -0.4 2,521,510 + MIT1002_02265 0.39 -0.2 2,521,518 - MIT1002_02265 0.40 -0.3 2,521,518 - MIT1002_02265 0.40 +0.2 2,521,568 + MIT1002_02265 0.42 +0.2 2,521,576 - MIT1002_02265 0.42 +0.3 2,521,610 + MIT1002_02265 0.43 -1.2 2,521,624 - MIT1002_02265 0.44 -0.8 2,521,630 + MIT1002_02265 0.44 -0.8 2,521,630 + MIT1002_02265 0.44 +0.3 2,521,700 + MIT1002_02265 0.47 +0.8 2,521,751 + MIT1002_02265 0.49 -0.7 2,521,761 + MIT1002_02265 0.49 +1.3 2,521,775 - MIT1002_02265 0.50 +0.5 2,521,781 - MIT1002_02265 0.50 -0.3 2,521,791 - MIT1002_02265 0.51 -0.3 2,521,818 + MIT1002_02265 0.52 -0.7 2,521,834 + MIT1002_02265 0.52 -1.9 2,521,834 + MIT1002_02265 0.52 -0.9 2,521,834 + MIT1002_02265 0.52 -0.9 2,521,842 - MIT1002_02265 0.53 -1.2 2,521,902 + MIT1002_02265 0.55 -0.2 2,521,907 + MIT1002_02265 0.55 +0.6 2,521,993 + MIT1002_02265 0.59 +0.2 2,522,049 + MIT1002_02265 0.61 +0.0 2,522,049 + MIT1002_02265 0.61 -1.0 2,522,051 - MIT1002_02265 0.61 +0.7 2,522,074 + MIT1002_02265 0.62 +1.5 2,522,108 + MIT1002_02265 0.64 +0.7 2,522,135 - MIT1002_02265 0.65 -0.0 2,522,162 - MIT1002_02265 0.66 -0.7 2,522,165 - MIT1002_02265 0.66 +1.3 2,522,206 + MIT1002_02265 0.67 -1.5 2,522,214 - MIT1002_02265 0.68 -0.1 2,522,223 + MIT1002_02265 0.68 -0.7 2,522,231 + MIT1002_02265 0.68 -0.3 2,522,246 - MIT1002_02265 0.69 -0.2 2,522,248 + MIT1002_02265 0.69 -0.7 2,522,256 - MIT1002_02265 0.70 -0.2 2,522,256 - MIT1002_02265 0.70 +1.6 2,522,256 - MIT1002_02265 0.70 -0.1 2,522,284 - MIT1002_02265 0.71 +1.4 2,522,344 - MIT1002_02265 0.73 +0.0 2,522,344 - MIT1002_02265 0.73 -1.5 2,522,369 + MIT1002_02265 0.74 -0.0 2,522,371 + MIT1002_02265 0.74 +0.4 2,522,371 + MIT1002_02265 0.74 -0.2 2,522,379 - MIT1002_02265 0.74 -0.7 2,522,379 - MIT1002_02265 0.74 +0.6 2,522,381 + MIT1002_02265 0.75 -1.7 2,522,440 + MIT1002_02265 0.77 -0.8 2,522,440 + MIT1002_02265 0.77 -0.1 2,522,448 - MIT1002_02265 0.77 -0.3 2,522,448 - MIT1002_02265 0.77 +0.2 2,522,495 + MIT1002_02265 0.79 +0.6 2,522,540 - MIT1002_02265 0.81 +0.9 2,522,602 + MIT1002_02265 0.84 -1.8 2,522,610 - MIT1002_02265 0.84 +0.5 2,522,706 - MIT1002_02265 0.88 -0.4 2,522,708 - MIT1002_02265 0.88 +1.6 2,522,763 + +1.3 2,522,818 - +0.5 2,522,871 + +0.1 2,522,881 + +0.3 2,522,881 + -0.1 2,522,889 - +0.2 2,523,118 + -1.3 2,523,147 - -3.1 2,523,156 + -3.4 2,523,164 - -0.7 2,523,176 - -3.4 2,523,176 - -1.8 2,523,176 - -2.2 2,523,176 - -3.1 2,523,205 - -4.6 2,523,225 + -1.4 2,523,233 - -3.7 2,523,365 - MIT1002_02266 0.20 -4.6 2,523,495 - MIT1002_02266 0.30 -0.8 2,523,502 - MIT1002_02266 0.31 -1.8 2,523,530 - MIT1002_02266 0.33 -5.2 2,523,627 + MIT1002_02266 0.41 -2.1 2,523,653 + MIT1002_02266 0.43 -0.8 2,523,653 + MIT1002_02266 0.43 -2.6 2,523,661 - MIT1002_02266 0.44 -4.4 2,523,679 + MIT1002_02266 0.45 -3.2 2,523,689 + MIT1002_02266 0.46 -1.8 2,523,818 + MIT1002_02266 0.56 -3.7 2,523,818 + MIT1002_02266 0.56 -5.0 2,523,826 - MIT1002_02266 0.57 -1.2 2,523,887 + MIT1002_02266 0.62 -4.1 2,523,899 + MIT1002_02266 0.63 -2.3 2,523,963 + MIT1002_02266 0.68 -2.2 2,523,963 + MIT1002_02266 0.68 -3.0 2,523,963 + MIT1002_02266 0.68 -2.3 2,523,963 + MIT1002_02266 0.68 -3.8 2,523,966 - MIT1002_02266 0.68 -4.7 2,523,971 - MIT1002_02266 0.69 -3.7
Or see this region's nucleotide sequence