Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02146 and MIT1002_02147 are separated by 122 nucleotides MIT1002_02147 and MIT1002_02148 are separated by 74 nucleotides MIT1002_02148 and MIT1002_02149 are separated by 128 nucleotides MIT1002_02149 and MIT1002_02150 are separated by 59 nucleotides MIT1002_02150 and MIT1002_02151 are separated by 41 nucleotides MIT1002_02151 and MIT1002_02152 are separated by 169 nucleotides
MIT1002_02146: MIT1002_02146 - 3-mercaptopyruvate sulfurtransferase, at 2,405,745 to 2,406,575
_02146
MIT1002_02147: MIT1002_02147 - putative thiol peroxidase, at 2,406,698 to 2,407,195
_02147
MIT1002_02148: MIT1002_02148 - hypothetical protein, at 2,407,270 to 2,407,962
_02148
MIT1002_02149: MIT1002_02149 - tRNA-Leu, at 2,408,091 to 2,408,177
_02149
MIT1002_02150: MIT1002_02150 - tRNA-Cys, at 2,408,237 to 2,408,310
_02150
MIT1002_02151: MIT1002_02151 - tRNA-Gly, at 2,408,352 to 2,408,427
_02151
MIT1002_02152: MIT1002_02152 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 2,408,597 to 2,409,169
_02152
Position (kb)
2407
2408 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2406.329 kb on + strand, within MIT1002_02146 at 2406.339 kb on - strand, within MIT1002_02146 at 2406.339 kb on - strand, within MIT1002_02146 at 2406.384 kb on - strand, within MIT1002_02146 at 2406.394 kb on + strand, within MIT1002_02146 at 2406.394 kb on + strand, within MIT1002_02146 at 2406.400 kb on + strand, within MIT1002_02146 at 2406.402 kb on - strand, within MIT1002_02146 at 2406.411 kb on + strand, within MIT1002_02146 at 2406.445 kb on + strand, within MIT1002_02146 at 2406.452 kb on + strand, within MIT1002_02146 at 2406.553 kb on + strand at 2406.598 kb on + strand at 2406.613 kb on + strand at 2406.615 kb on + strand at 2406.673 kb on + strand at 2406.678 kb on + strand at 2406.701 kb on + strand at 2406.706 kb on + strand at 2406.709 kb on - strand at 2406.762 kb on + strand, within MIT1002_02147 at 2406.762 kb on + strand, within MIT1002_02147 at 2406.769 kb on + strand, within MIT1002_02147 at 2406.770 kb on - strand, within MIT1002_02147 at 2406.770 kb on - strand, within MIT1002_02147 at 2406.770 kb on - strand, within MIT1002_02147 at 2406.793 kb on + strand, within MIT1002_02147 at 2406.904 kb on + strand, within MIT1002_02147 at 2406.910 kb on - strand, within MIT1002_02147 at 2406.912 kb on - strand, within MIT1002_02147 at 2406.961 kb on + strand, within MIT1002_02147 at 2406.961 kb on + strand, within MIT1002_02147 at 2406.966 kb on - strand, within MIT1002_02147 at 2406.968 kb on - strand, within MIT1002_02147 at 2406.969 kb on - strand, within MIT1002_02147 at 2406.969 kb on - strand, within MIT1002_02147 at 2407.054 kb on - strand, within MIT1002_02147 at 2407.071 kb on + strand, within MIT1002_02147 at 2407.081 kb on + strand, within MIT1002_02147 at 2407.089 kb on - strand, within MIT1002_02147 at 2407.089 kb on - strand, within MIT1002_02147 at 2407.089 kb on - strand, within MIT1002_02147 at 2407.126 kb on - strand, within MIT1002_02147 at 2407.131 kb on - strand, within MIT1002_02147 at 2407.306 kb on - strand at 2407.364 kb on - strand, within MIT1002_02148 at 2407.376 kb on + strand, within MIT1002_02148 at 2407.395 kb on + strand, within MIT1002_02148 at 2407.395 kb on + strand, within MIT1002_02148 at 2407.398 kb on - strand, within MIT1002_02148 at 2407.423 kb on + strand, within MIT1002_02148 at 2407.624 kb on + strand, within MIT1002_02148 at 2407.632 kb on - strand, within MIT1002_02148 at 2407.944 kb on - strand at 2407.952 kb on - strand at 2408.085 kb on + strand at 2408.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 2,406,329 + MIT1002_02146 0.70 +0.0 2,406,339 - MIT1002_02146 0.71 +1.2 2,406,339 - MIT1002_02146 0.71 +1.0 2,406,384 - MIT1002_02146 0.77 +0.6 2,406,394 + MIT1002_02146 0.78 -0.3 2,406,394 + MIT1002_02146 0.78 -0.6 2,406,400 + MIT1002_02146 0.79 +0.4 2,406,402 - MIT1002_02146 0.79 +0.7 2,406,411 + MIT1002_02146 0.80 +0.1 2,406,445 + MIT1002_02146 0.84 +1.4 2,406,452 + MIT1002_02146 0.85 -1.2 2,406,553 + +0.2 2,406,598 + +0.4 2,406,613 + +0.3 2,406,615 + +0.0 2,406,673 + -1.7 2,406,678 + -0.7 2,406,701 + +0.7 2,406,706 + -0.2 2,406,709 - -1.3 2,406,762 + MIT1002_02147 0.13 -0.8 2,406,762 + MIT1002_02147 0.13 +0.0 2,406,769 + MIT1002_02147 0.14 +0.1 2,406,770 - MIT1002_02147 0.14 -0.5 2,406,770 - MIT1002_02147 0.14 +0.9 2,406,770 - MIT1002_02147 0.14 +0.6 2,406,793 + MIT1002_02147 0.19 +0.3 2,406,904 + MIT1002_02147 0.41 +0.9 2,406,910 - MIT1002_02147 0.43 +1.0 2,406,912 - MIT1002_02147 0.43 -0.5 2,406,961 + MIT1002_02147 0.53 -0.7 2,406,961 + MIT1002_02147 0.53 -0.9 2,406,966 - MIT1002_02147 0.54 +0.3 2,406,968 - MIT1002_02147 0.54 +0.4 2,406,969 - MIT1002_02147 0.54 -0.3 2,406,969 - MIT1002_02147 0.54 -0.6 2,407,054 - MIT1002_02147 0.71 +0.9 2,407,071 + MIT1002_02147 0.75 -0.1 2,407,081 + MIT1002_02147 0.77 -0.4 2,407,089 - MIT1002_02147 0.79 +2.5 2,407,089 - MIT1002_02147 0.79 -0.1 2,407,089 - MIT1002_02147 0.79 +1.0 2,407,126 - MIT1002_02147 0.86 +0.6 2,407,131 - MIT1002_02147 0.87 +0.9 2,407,306 - +1.7 2,407,364 - MIT1002_02148 0.14 -1.3 2,407,376 + MIT1002_02148 0.15 +2.1 2,407,395 + MIT1002_02148 0.18 -0.9 2,407,395 + MIT1002_02148 0.18 -0.4 2,407,398 - MIT1002_02148 0.18 +2.1 2,407,423 + MIT1002_02148 0.22 +2.9 2,407,624 + MIT1002_02148 0.51 -1.6 2,407,632 - MIT1002_02148 0.52 +0.3 2,407,944 - +0.5 2,407,952 - +0.1 2,408,085 + +0.3 2,408,628 - -2.6
Or see this region's nucleotide sequence