Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01224 and MIT1002_01225 are separated by 98 nucleotides MIT1002_01225 and MIT1002_01226 are separated by 178 nucleotides MIT1002_01226 and MIT1002_01227 are separated by 29 nucleotides MIT1002_01227 and MIT1002_01228 are separated by 152 nucleotides
MIT1002_01224: MIT1002_01224 - hypothetical protein, at 1,348,982 to 1,349,440
_01224
MIT1002_01225: MIT1002_01225 - hypothetical protein, at 1,349,539 to 1,349,952
_01225
MIT1002_01226: MIT1002_01226 - hypothetical protein, at 1,350,131 to 1,350,256
_01226
MIT1002_01227: MIT1002_01227 - recombination and DNA strand exchange inhibitor protein, at 1,350,286 to 1,350,936
_01227
MIT1002_01228: MIT1002_01228 - 50S ribosomal protein L3 glutamine methyltransferase, at 1,351,089 to 1,352,024
_01228
Position (kb)
1350
1351 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1349.327 kb on - strand, within MIT1002_01224 at 1349.366 kb on + strand, within MIT1002_01224 at 1349.376 kb on - strand, within MIT1002_01224 at 1349.427 kb on - strand at 1349.439 kb on + strand at 1349.447 kb on - strand at 1349.447 kb on - strand at 1349.447 kb on - strand at 1349.449 kb on + strand at 1349.494 kb on + strand at 1349.494 kb on + strand at 1349.502 kb on - strand at 1349.502 kb on - strand at 1349.522 kb on - strand at 1349.604 kb on - strand, within MIT1002_01225 at 1349.616 kb on - strand, within MIT1002_01225 at 1349.729 kb on + strand, within MIT1002_01225 at 1349.754 kb on - strand, within MIT1002_01225 at 1349.836 kb on - strand, within MIT1002_01225 at 1349.886 kb on + strand, within MIT1002_01225 at 1350.018 kb on - strand at 1350.018 kb on - strand at 1350.050 kb on - strand at 1350.183 kb on - strand, within MIT1002_01226 at 1350.188 kb on - strand, within MIT1002_01226 at 1350.227 kb on + strand, within MIT1002_01226 at 1350.235 kb on - strand, within MIT1002_01226 at 1350.245 kb on - strand at 1350.263 kb on + strand at 1350.273 kb on + strand at 1350.385 kb on + strand, within MIT1002_01227 at 1350.424 kb on + strand, within MIT1002_01227 at 1350.427 kb on - strand, within MIT1002_01227 at 1350.449 kb on - strand, within MIT1002_01227 at 1350.465 kb on + strand, within MIT1002_01227 at 1350.519 kb on + strand, within MIT1002_01227 at 1350.528 kb on - strand, within MIT1002_01227 at 1350.528 kb on - strand, within MIT1002_01227 at 1350.649 kb on - strand, within MIT1002_01227 at 1350.649 kb on - strand, within MIT1002_01227 at 1350.661 kb on + strand, within MIT1002_01227 at 1350.755 kb on + strand, within MIT1002_01227 at 1350.763 kb on - strand, within MIT1002_01227 at 1350.773 kb on - strand, within MIT1002_01227 at 1350.784 kb on + strand, within MIT1002_01227 at 1350.784 kb on + strand, within MIT1002_01227 at 1350.909 kb on - strand at 1350.916 kb on - strand at 1350.950 kb on + strand at 1351.023 kb on - strand at 1351.288 kb on + strand, within MIT1002_01228 at 1351.373 kb on + strand, within MIT1002_01228 at 1351.373 kb on + strand, within MIT1002_01228 at 1351.417 kb on + strand, within MIT1002_01228 at 1351.592 kb on + strand, within MIT1002_01228 at 1351.642 kb on + strand, within MIT1002_01228 at 1351.642 kb on + strand, within MIT1002_01228 at 1351.745 kb on + strand, within MIT1002_01228 at 1351.745 kb on + strand, within MIT1002_01228 at 1351.745 kb on + strand, within MIT1002_01228 at 1351.803 kb on + strand, within MIT1002_01228 at 1351.803 kb on + strand, within MIT1002_01228 at 1351.803 kb on + strand, within MIT1002_01228 at 1351.813 kb on + strand, within MIT1002_01228
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 1,349,327 - MIT1002_01224 0.75 -0.8 1,349,366 + MIT1002_01224 0.84 -1.2 1,349,376 - MIT1002_01224 0.86 +0.8 1,349,427 - -0.1 1,349,439 + +1.2 1,349,447 - +1.9 1,349,447 - +0.3 1,349,447 - +1.3 1,349,449 + +0.3 1,349,494 + +1.6 1,349,494 + +0.6 1,349,502 - +0.2 1,349,502 - +1.3 1,349,522 - -0.2 1,349,604 - MIT1002_01225 0.16 -0.6 1,349,616 - MIT1002_01225 0.19 -0.6 1,349,729 + MIT1002_01225 0.46 +0.1 1,349,754 - MIT1002_01225 0.52 -0.2 1,349,836 - MIT1002_01225 0.72 +0.1 1,349,886 + MIT1002_01225 0.84 -0.3 1,350,018 - +0.5 1,350,018 - -1.0 1,350,050 - -1.9 1,350,183 - MIT1002_01226 0.41 -0.1 1,350,188 - MIT1002_01226 0.45 -0.9 1,350,227 + MIT1002_01226 0.76 +1.4 1,350,235 - MIT1002_01226 0.83 +1.6 1,350,245 - +0.5 1,350,263 + +0.3 1,350,273 + +0.3 1,350,385 + MIT1002_01227 0.15 +1.3 1,350,424 + MIT1002_01227 0.21 +0.8 1,350,427 - MIT1002_01227 0.22 +1.5 1,350,449 - MIT1002_01227 0.25 +0.8 1,350,465 + MIT1002_01227 0.27 -0.7 1,350,519 + MIT1002_01227 0.36 -0.0 1,350,528 - MIT1002_01227 0.37 -0.2 1,350,528 - MIT1002_01227 0.37 -1.6 1,350,649 - MIT1002_01227 0.56 +0.8 1,350,649 - MIT1002_01227 0.56 +1.6 1,350,661 + MIT1002_01227 0.58 +0.0 1,350,755 + MIT1002_01227 0.72 +0.9 1,350,763 - MIT1002_01227 0.73 -0.7 1,350,773 - MIT1002_01227 0.75 +0.4 1,350,784 + MIT1002_01227 0.76 -0.4 1,350,784 + MIT1002_01227 0.76 +0.2 1,350,909 - -0.4 1,350,916 - -0.5 1,350,950 + -1.0 1,351,023 - +0.6 1,351,288 + MIT1002_01228 0.21 -1.2 1,351,373 + MIT1002_01228 0.30 +1.2 1,351,373 + MIT1002_01228 0.30 -1.0 1,351,417 + MIT1002_01228 0.35 -1.2 1,351,592 + MIT1002_01228 0.54 +0.4 1,351,642 + MIT1002_01228 0.59 +1.2 1,351,642 + MIT1002_01228 0.59 +0.2 1,351,745 + MIT1002_01228 0.70 -0.5 1,351,745 + MIT1002_01228 0.70 +0.3 1,351,745 + MIT1002_01228 0.70 +0.6 1,351,803 + MIT1002_01228 0.76 -0.6 1,351,803 + MIT1002_01228 0.76 +0.8 1,351,803 + MIT1002_01228 0.76 -1.1 1,351,813 + MIT1002_01228 0.77 -1.1
Or see this region's nucleotide sequence