Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00657 and MIT1002_00658 are separated by 149 nucleotides MIT1002_00658 and MIT1002_00659 are separated by 49 nucleotides
MIT1002_00657: MIT1002_00657 - hypothetical protein, at 713,816 to 714,703
_00657
MIT1002_00658: MIT1002_00658 - Ribosomal large subunit pseudouridine synthase A, at 714,853 to 715,569
_00658
MIT1002_00659: MIT1002_00659 - RNA polymerase-associated protein RapA, at 715,619 to 718,480
_00659
Position (kb)
714
715
716 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 713.993 kb on - strand, within MIT1002_00657 at 713.996 kb on - strand, within MIT1002_00657 at 713.999 kb on + strand, within MIT1002_00657 at 714.046 kb on + strand, within MIT1002_00657 at 714.122 kb on - strand, within MIT1002_00657 at 714.125 kb on + strand, within MIT1002_00657 at 714.159 kb on + strand, within MIT1002_00657 at 714.167 kb on - strand, within MIT1002_00657 at 714.167 kb on - strand, within MIT1002_00657 at 714.175 kb on - strand, within MIT1002_00657 at 714.176 kb on - strand, within MIT1002_00657 at 714.176 kb on - strand, within MIT1002_00657 at 714.178 kb on + strand, within MIT1002_00657 at 714.180 kb on + strand, within MIT1002_00657 at 714.187 kb on + strand, within MIT1002_00657 at 714.195 kb on - strand, within MIT1002_00657 at 714.195 kb on - strand, within MIT1002_00657 at 714.204 kb on - strand, within MIT1002_00657 at 714.247 kb on + strand, within MIT1002_00657 at 714.294 kb on + strand, within MIT1002_00657 at 714.302 kb on - strand, within MIT1002_00657 at 714.326 kb on - strand, within MIT1002_00657 at 714.338 kb on - strand, within MIT1002_00657 at 714.377 kb on - strand, within MIT1002_00657 at 714.524 kb on + strand, within MIT1002_00657 at 714.525 kb on + strand, within MIT1002_00657 at 714.525 kb on - strand, within MIT1002_00657 at 714.532 kb on - strand, within MIT1002_00657 at 714.578 kb on + strand, within MIT1002_00657 at 714.586 kb on - strand, within MIT1002_00657 at 714.622 kb on - strand at 714.645 kb on + strand at 714.645 kb on + strand at 714.653 kb on - strand at 714.653 kb on - strand at 714.678 kb on + strand at 714.678 kb on + strand at 714.685 kb on + strand at 714.711 kb on + strand at 714.728 kb on + strand at 714.780 kb on + strand at 714.788 kb on - strand at 714.970 kb on - strand, within MIT1002_00658 at 714.996 kb on - strand, within MIT1002_00658 at 715.068 kb on - strand, within MIT1002_00658 at 715.098 kb on + strand, within MIT1002_00658 at 715.137 kb on + strand, within MIT1002_00658 at 715.199 kb on - strand, within MIT1002_00658 at 715.199 kb on - strand, within MIT1002_00658 at 715.226 kb on + strand, within MIT1002_00658 at 715.234 kb on - strand, within MIT1002_00658 at 715.234 kb on - strand, within MIT1002_00658 at 715.235 kb on - strand, within MIT1002_00658 at 715.236 kb on - strand, within MIT1002_00658 at 715.251 kb on - strand, within MIT1002_00658 at 715.366 kb on + strand, within MIT1002_00658 at 715.380 kb on - strand, within MIT1002_00658 at 715.381 kb on + strand, within MIT1002_00658 at 715.383 kb on + strand, within MIT1002_00658 at 715.389 kb on - strand, within MIT1002_00658 at 715.389 kb on - strand, within MIT1002_00658 at 715.391 kb on - strand, within MIT1002_00658 at 715.440 kb on + strand, within MIT1002_00658 at 715.441 kb on + strand, within MIT1002_00658 at 715.441 kb on + strand, within MIT1002_00658 at 715.448 kb on - strand, within MIT1002_00658 at 715.479 kb on + strand, within MIT1002_00658 at 715.486 kb on - strand, within MIT1002_00658 at 715.498 kb on + strand at 715.506 kb on - strand at 715.529 kb on + strand at 715.591 kb on - strand at 715.609 kb on - strand at 715.618 kb on - strand at 715.662 kb on + strand at 715.753 kb on - strand at 715.755 kb on - strand at 715.798 kb on - strand at 715.799 kb on + strand at 715.974 kb on - strand, within MIT1002_00659 at 716.139 kb on - strand, within MIT1002_00659 at 716.224 kb on + strand, within MIT1002_00659 at 716.240 kb on - strand, within MIT1002_00659 at 716.328 kb on - strand, within MIT1002_00659 at 716.385 kb on - strand, within MIT1002_00659 at 716.411 kb on + strand, within MIT1002_00659 at 716.411 kb on + strand, within MIT1002_00659 at 716.419 kb on - strand, within MIT1002_00659 at 716.419 kb on - strand, within MIT1002_00659 at 716.424 kb on + strand, within MIT1002_00659 at 716.454 kb on + strand, within MIT1002_00659 at 716.532 kb on + strand, within MIT1002_00659 at 716.534 kb on + strand, within MIT1002_00659 at 716.542 kb on - strand, within MIT1002_00659 at 716.555 kb on - strand, within MIT1002_00659 at 716.555 kb on - strand, within MIT1002_00659 at 716.562 kb on - strand, within MIT1002_00659 at 716.567 kb on - strand, within MIT1002_00659
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 713,993 - MIT1002_00657 0.20 +3.4 713,996 - MIT1002_00657 0.20 -0.2 713,999 + MIT1002_00657 0.21 +0.3 714,046 + MIT1002_00657 0.26 +0.0 714,122 - MIT1002_00657 0.34 -0.2 714,125 + MIT1002_00657 0.35 +0.2 714,159 + MIT1002_00657 0.39 -1.9 714,167 - MIT1002_00657 0.40 +0.2 714,167 - MIT1002_00657 0.40 +1.0 714,175 - MIT1002_00657 0.40 -0.9 714,176 - MIT1002_00657 0.41 -1.0 714,176 - MIT1002_00657 0.41 -2.0 714,178 + MIT1002_00657 0.41 +1.0 714,180 + MIT1002_00657 0.41 +0.3 714,187 + MIT1002_00657 0.42 +0.9 714,195 - MIT1002_00657 0.43 -0.0 714,195 - MIT1002_00657 0.43 +0.4 714,204 - MIT1002_00657 0.44 -0.2 714,247 + MIT1002_00657 0.49 -0.3 714,294 + MIT1002_00657 0.54 -1.7 714,302 - MIT1002_00657 0.55 +0.2 714,326 - MIT1002_00657 0.57 +0.8 714,338 - MIT1002_00657 0.59 -2.2 714,377 - MIT1002_00657 0.63 -0.8 714,524 + MIT1002_00657 0.80 +1.2 714,525 + MIT1002_00657 0.80 -0.2 714,525 - MIT1002_00657 0.80 -0.1 714,532 - MIT1002_00657 0.81 -2.9 714,578 + MIT1002_00657 0.86 +0.1 714,586 - MIT1002_00657 0.87 -0.6 714,622 - -0.5 714,645 + -0.1 714,645 + -1.4 714,653 - -0.7 714,653 - -0.0 714,678 + -2.4 714,678 + -1.8 714,685 + +0.8 714,711 + +1.4 714,728 + -0.8 714,780 + -0.8 714,788 - +1.5 714,970 - MIT1002_00658 0.16 +1.8 714,996 - MIT1002_00658 0.20 -0.8 715,068 - MIT1002_00658 0.30 +0.0 715,098 + MIT1002_00658 0.34 -1.3 715,137 + MIT1002_00658 0.40 -1.0 715,199 - MIT1002_00658 0.48 +0.2 715,199 - MIT1002_00658 0.48 -1.3 715,226 + MIT1002_00658 0.52 -0.2 715,234 - MIT1002_00658 0.53 -1.3 715,234 - MIT1002_00658 0.53 +0.5 715,235 - MIT1002_00658 0.53 -0.7 715,236 - MIT1002_00658 0.53 -0.7 715,251 - MIT1002_00658 0.56 -3.2 715,366 + MIT1002_00658 0.72 +0.5 715,380 - MIT1002_00658 0.74 +0.8 715,381 + MIT1002_00658 0.74 -0.2 715,383 + MIT1002_00658 0.74 -0.2 715,389 - MIT1002_00658 0.75 -0.4 715,389 - MIT1002_00658 0.75 +0.7 715,391 - MIT1002_00658 0.75 -0.8 715,440 + MIT1002_00658 0.82 -0.3 715,441 + MIT1002_00658 0.82 +1.1 715,441 + MIT1002_00658 0.82 -0.6 715,448 - MIT1002_00658 0.83 -0.4 715,479 + MIT1002_00658 0.87 +0.5 715,486 - MIT1002_00658 0.88 -1.6 715,498 + +0.5 715,506 - -0.0 715,529 + -1.8 715,591 - -0.4 715,609 - -0.7 715,618 - -0.7 715,662 + +0.3 715,753 - -0.1 715,755 - +0.4 715,798 - +0.1 715,799 + +1.5 715,974 - MIT1002_00659 0.12 +1.9 716,139 - MIT1002_00659 0.18 -1.7 716,224 + MIT1002_00659 0.21 +0.1 716,240 - MIT1002_00659 0.22 -0.5 716,328 - MIT1002_00659 0.25 +0.9 716,385 - MIT1002_00659 0.27 +0.7 716,411 + MIT1002_00659 0.28 -1.3 716,411 + MIT1002_00659 0.28 +0.8 716,419 - MIT1002_00659 0.28 -2.2 716,419 - MIT1002_00659 0.28 +0.2 716,424 + MIT1002_00659 0.28 +0.3 716,454 + MIT1002_00659 0.29 +0.1 716,532 + MIT1002_00659 0.32 +0.7 716,534 + MIT1002_00659 0.32 +0.1 716,542 - MIT1002_00659 0.32 +0.5 716,555 - MIT1002_00659 0.33 +1.2 716,555 - MIT1002_00659 0.33 +0.2 716,562 - MIT1002_00659 0.33 -0.9 716,567 - MIT1002_00659 0.33 -0.4
Or see this region's nucleotide sequence