Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00036 and MIT1002_00037 are separated by 140 nucleotides MIT1002_00037 and MIT1002_00038 are separated by 136 nucleotides MIT1002_00038 and MIT1002_00039 overlap by 20 nucleotides
MIT1002_00036: MIT1002_00036 - Transposase, at 40,196 to 40,711
_00036
MIT1002_00037: MIT1002_00037 - Lipid A 3-O-deacylase (PagL), at 40,852 to 41,382
_00037
MIT1002_00038: MIT1002_00038 - hypothetical protein, at 41,519 to 42,349
_00038
MIT1002_00039: MIT1002_00039 - hypothetical protein, at 42,330 to 43,121
_00039
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 40.802 kb on + strand at 40.810 kb on - strand at 40.865 kb on + strand at 40.873 kb on - strand at 40.873 kb on - strand at 40.884 kb on + strand at 40.977 kb on + strand, within MIT1002_00037 at 41.002 kb on + strand, within MIT1002_00037 at 41.051 kb on + strand, within MIT1002_00037 at 41.104 kb on - strand, within MIT1002_00037 at 41.147 kb on - strand, within MIT1002_00037 at 41.210 kb on - strand, within MIT1002_00037 at 41.233 kb on - strand, within MIT1002_00037 at 41.481 kb on + strand at 41.509 kb on + strand at 41.570 kb on - strand at 41.652 kb on - strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.680 kb on + strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.688 kb on - strand, within MIT1002_00038 at 41.714 kb on + strand, within MIT1002_00038 at 41.722 kb on + strand, within MIT1002_00038 at 41.723 kb on + strand, within MIT1002_00038 at 41.723 kb on + strand, within MIT1002_00038 at 41.731 kb on - strand, within MIT1002_00038 at 41.731 kb on - strand, within MIT1002_00038 at 41.747 kb on + strand, within MIT1002_00038 at 41.767 kb on - strand, within MIT1002_00038 at 41.781 kb on + strand, within MIT1002_00038 at 41.802 kb on - strand, within MIT1002_00038 at 41.827 kb on + strand, within MIT1002_00038 at 41.870 kb on - strand, within MIT1002_00038 at 42.017 kb on - strand, within MIT1002_00038 at 42.026 kb on - strand, within MIT1002_00038 at 42.040 kb on + strand, within MIT1002_00038 at 42.099 kb on + strand, within MIT1002_00038 at 42.107 kb on + strand, within MIT1002_00038 at 42.174 kb on + strand, within MIT1002_00038 at 42.174 kb on + strand, within MIT1002_00038 at 42.177 kb on - strand, within MIT1002_00038 at 42.187 kb on - strand, within MIT1002_00038 at 42.187 kb on - strand, within MIT1002_00038 at 42.187 kb on - strand, within MIT1002_00038 at 42.249 kb on - strand, within MIT1002_00038 at 42.254 kb on + strand, within MIT1002_00038 at 42.299 kb on + strand at 42.367 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 40,802 + +0.1 40,810 - +1.0 40,865 + +0.1 40,873 - -0.2 40,873 - +0.2 40,884 + +2.8 40,977 + MIT1002_00037 0.24 +0.8 41,002 + MIT1002_00037 0.28 -1.6 41,051 + MIT1002_00037 0.37 -0.3 41,104 - MIT1002_00037 0.47 -1.1 41,147 - MIT1002_00037 0.56 -1.5 41,210 - MIT1002_00037 0.67 +1.0 41,233 - MIT1002_00037 0.72 +0.2 41,481 + +0.3 41,509 + +0.4 41,570 - +1.9 41,652 - MIT1002_00038 0.16 -0.4 41,680 + MIT1002_00038 0.19 -0.1 41,680 + MIT1002_00038 0.19 -1.3 41,680 + MIT1002_00038 0.19 -0.1 41,680 + MIT1002_00038 0.19 +0.0 41,680 + MIT1002_00038 0.19 -1.9 41,680 + MIT1002_00038 0.19 +0.2 41,680 + MIT1002_00038 0.19 -0.3 41,680 + MIT1002_00038 0.19 -0.2 41,680 + MIT1002_00038 0.19 -1.8 41,680 + MIT1002_00038 0.19 -0.7 41,688 - MIT1002_00038 0.20 +0.4 41,688 - MIT1002_00038 0.20 -1.2 41,688 - MIT1002_00038 0.20 -0.4 41,688 - MIT1002_00038 0.20 -1.2 41,688 - MIT1002_00038 0.20 -0.7 41,688 - MIT1002_00038 0.20 -0.5 41,714 + MIT1002_00038 0.23 +0.2 41,722 + MIT1002_00038 0.24 +0.0 41,723 + MIT1002_00038 0.25 -0.1 41,723 + MIT1002_00038 0.25 +0.1 41,731 - MIT1002_00038 0.26 -1.1 41,731 - MIT1002_00038 0.26 -1.2 41,747 + MIT1002_00038 0.27 -1.0 41,767 - MIT1002_00038 0.30 +0.2 41,781 + MIT1002_00038 0.32 -0.3 41,802 - MIT1002_00038 0.34 +1.3 41,827 + MIT1002_00038 0.37 +0.2 41,870 - MIT1002_00038 0.42 -0.3 42,017 - MIT1002_00038 0.60 -0.5 42,026 - MIT1002_00038 0.61 +1.8 42,040 + MIT1002_00038 0.63 -0.3 42,099 + MIT1002_00038 0.70 +0.7 42,107 + MIT1002_00038 0.71 -0.5 42,174 + MIT1002_00038 0.79 +0.5 42,174 + MIT1002_00038 0.79 -0.1 42,177 - MIT1002_00038 0.79 +0.5 42,187 - MIT1002_00038 0.80 +0.0 42,187 - MIT1002_00038 0.80 +0.1 42,187 - MIT1002_00038 0.80 +0.4 42,249 - MIT1002_00038 0.88 +0.5 42,254 + MIT1002_00038 0.88 -1.8 42,299 + +0.1 42,367 + +2.5
Or see this region's nucleotide sequence