Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00006 and MIT1002_00007 are separated by 2 nucleotides MIT1002_00007 and MIT1002_00008 are separated by 146 nucleotides MIT1002_00008 and MIT1002_00009 are separated by 112 nucleotides
MIT1002_00006: MIT1002_00006 - Glycine--tRNA ligase beta subunit, at 7,070 to 9,148
_00006
MIT1002_00007: MIT1002_00007 - Glycine--tRNA ligase alpha subunit, at 9,151 to 10,056
_00007
MIT1002_00008: MIT1002_00008 - DNA-3-methyladenine glycosylase 1, at 10,203 to 10,811
_00008
MIT1002_00009: MIT1002_00009 - hypothetical protein, at 10,924 to 11,385
_00009
Position (kb) 9
10
11 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 10.139 kb on - strand at 10.139 kb on - strand at 10.311 kb on + strand, within MIT1002_00008 at 10.312 kb on + strand, within MIT1002_00008 at 10.348 kb on - strand, within MIT1002_00008 at 10.365 kb on + strand, within MIT1002_00008 at 10.373 kb on - strand, within MIT1002_00008 at 10.373 kb on - strand, within MIT1002_00008 at 10.413 kb on + strand, within MIT1002_00008 at 10.424 kb on + strand, within MIT1002_00008 at 10.424 kb on + strand, within MIT1002_00008 at 10.424 kb on + strand, within MIT1002_00008 at 10.469 kb on - strand, within MIT1002_00008 at 10.469 kb on - strand, within MIT1002_00008 at 10.618 kb on - strand, within MIT1002_00008 at 10.618 kb on - strand, within MIT1002_00008 at 10.626 kb on + strand, within MIT1002_00008 at 10.635 kb on - strand, within MIT1002_00008 at 10.645 kb on + strand, within MIT1002_00008 at 10.658 kb on + strand, within MIT1002_00008 at 10.666 kb on - strand, within MIT1002_00008 at 10.666 kb on - strand, within MIT1002_00008 at 10.678 kb on - strand, within MIT1002_00008 at 10.736 kb on + strand, within MIT1002_00008 at 10.737 kb on + strand, within MIT1002_00008 at 10.745 kb on - strand, within MIT1002_00008 at 10.757 kb on + strand at 10.782 kb on + strand at 10.847 kb on + strand at 10.857 kb on + strand at 10.973 kb on - strand, within MIT1002_00009 at 10.983 kb on - strand, within MIT1002_00009 at 10.991 kb on - strand, within MIT1002_00009 at 11.053 kb on + strand, within MIT1002_00009
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 10,139 - +0.2 10,139 - -0.8 10,311 + MIT1002_00008 0.18 -1.9 10,312 + MIT1002_00008 0.18 -0.5 10,348 - MIT1002_00008 0.24 +0.8 10,365 + MIT1002_00008 0.27 -1.1 10,373 - MIT1002_00008 0.28 -0.6 10,373 - MIT1002_00008 0.28 -0.3 10,413 + MIT1002_00008 0.34 +0.7 10,424 + MIT1002_00008 0.36 +0.0 10,424 + MIT1002_00008 0.36 -0.3 10,424 + MIT1002_00008 0.36 -2.3 10,469 - MIT1002_00008 0.44 -2.1 10,469 - MIT1002_00008 0.44 -0.4 10,618 - MIT1002_00008 0.68 +1.8 10,618 - MIT1002_00008 0.68 +0.0 10,626 + MIT1002_00008 0.69 -0.8 10,635 - MIT1002_00008 0.71 -0.6 10,645 + MIT1002_00008 0.73 +1.4 10,658 + MIT1002_00008 0.75 +0.1 10,666 - MIT1002_00008 0.76 -0.8 10,666 - MIT1002_00008 0.76 -0.1 10,678 - MIT1002_00008 0.78 -0.3 10,736 + MIT1002_00008 0.88 +1.7 10,737 + MIT1002_00008 0.88 +0.1 10,745 - MIT1002_00008 0.89 +0.1 10,757 + +0.6 10,782 + +1.2 10,847 + -3.0 10,857 + +0.3 10,973 - MIT1002_00009 0.11 -1.4 10,983 - MIT1002_00009 0.13 +0.8 10,991 - MIT1002_00009 0.15 +0.3 11,053 + MIT1002_00009 0.28 -1.7
Or see this region's nucleotide sequence