Experiment: biofilm; Day2; NLDM;
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12280 and LRK53_RS12285 are separated by 176 nucleotides LRK53_RS12285 and LRK53_RS12290 overlap by 4 nucleotides LRK53_RS12290 and LRK53_RS12295 overlap by 1 nucleotides
LRK53_RS12280: LRK53_RS12280 - tRNA pseudouridine(13) synthase TruD, at 2,724,742 to 2,725,752
_RS12280
LRK53_RS12285: LRK53_RS12285 - magnesium transporter, at 2,725,929 to 2,727,323
_RS12285
LRK53_RS12290: LRK53_RS12290 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,727,320 to 2,727,793
_RS12290
LRK53_RS12295: LRK53_RS12295 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,727,793 to 2,728,488
_RS12295
Position (kb)
2725
2726
2727
2728 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2725.283 kb on + strand, within LRK53_RS12280 at 2725.283 kb on + strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.736 kb on + strand at 2725.736 kb on + strand at 2725.736 kb on + strand at 2725.737 kb on - strand at 2725.737 kb on - strand at 2725.761 kb on + strand at 2725.761 kb on + strand at 2725.762 kb on - strand at 2725.762 kb on - strand at 2725.762 kb on - strand at 2725.769 kb on + strand at 2725.923 kb on + strand at 2725.923 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.930 kb on + strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2725.931 kb on - strand at 2726.134 kb on + strand, within LRK53_RS12285 at 2726.135 kb on - strand, within LRK53_RS12285 at 2726.600 kb on - strand, within LRK53_RS12285 at 2726.600 kb on - strand, within LRK53_RS12285 at 2726.626 kb on + strand, within LRK53_RS12285 at 2726.627 kb on - strand, within LRK53_RS12285 at 2726.627 kb on - strand, within LRK53_RS12285 at 2726.887 kb on + strand, within LRK53_RS12285 at 2726.888 kb on - strand, within LRK53_RS12285 at 2726.888 kb on - strand, within LRK53_RS12285 at 2727.201 kb on + strand at 2727.201 kb on + strand at 2727.201 kb on + strand at 2727.201 kb on + strand at 2727.202 kb on - strand at 2727.202 kb on - strand at 2727.786 kb on - strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.794 kb on + strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand at 2727.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction biofilm; Day2; NLDM; remove 2,725,283 + LRK53_RS12280 0.54 +0.1 2,725,283 + LRK53_RS12280 0.54 -0.5 2,725,284 - LRK53_RS12280 0.54 -1.0 2,725,284 - LRK53_RS12280 0.54 +1.1 2,725,284 - LRK53_RS12280 0.54 -1.5 2,725,284 - LRK53_RS12280 0.54 -0.8 2,725,284 - LRK53_RS12280 0.54 -1.1 2,725,736 + -1.6 2,725,736 + +0.2 2,725,736 + -1.1 2,725,737 - +0.4 2,725,737 - -0.5 2,725,761 + -1.2 2,725,761 + -1.2 2,725,762 - -0.3 2,725,762 - -0.9 2,725,762 - +1.4 2,725,769 + -0.2 2,725,923 + -1.4 2,725,923 + +0.6 2,725,930 + -0.6 2,725,930 + -0.1 2,725,930 + +1.6 2,725,930 + -2.2 2,725,930 + -2.0 2,725,930 + -0.4 2,725,930 + -0.1 2,725,930 + +0.4 2,725,930 + +1.4 2,725,930 + -0.5 2,725,930 + +1.6 2,725,930 + -2.2 2,725,930 + -1.8 2,725,930 + -2.0 2,725,930 + -0.9 2,725,930 + -0.1 2,725,931 - -1.7 2,725,931 - -0.6 2,725,931 - +1.3 2,725,931 - +1.1 2,725,931 - +0.4 2,725,931 - +0.1 2,725,931 - -1.6 2,725,931 - -1.0 2,725,931 - -3.2 2,725,931 - -1.6 2,725,931 - -0.7 2,725,931 - +0.1 2,725,931 - -2.3 2,725,931 - +2.9 2,725,931 - -2.1 2,725,931 - -0.8 2,725,931 - -1.0 2,725,931 - +1.7 2,725,931 - -1.3 2,725,931 - -2.0 2,725,931 - -2.3 2,725,931 - -3.2 2,726,134 + LRK53_RS12285 0.15 -0.3 2,726,135 - LRK53_RS12285 0.15 -0.2 2,726,600 - LRK53_RS12285 0.48 +0.5 2,726,600 - LRK53_RS12285 0.48 -1.3 2,726,626 + LRK53_RS12285 0.50 +0.4 2,726,627 - LRK53_RS12285 0.50 +0.6 2,726,627 - LRK53_RS12285 0.50 +0.1 2,726,887 + LRK53_RS12285 0.69 +1.4 2,726,888 - LRK53_RS12285 0.69 +1.4 2,726,888 - LRK53_RS12285 0.69 +0.9 2,727,201 + -0.1 2,727,201 + -1.4 2,727,201 + -0.3 2,727,201 + -0.6 2,727,202 - +0.2 2,727,202 - -1.9 2,727,786 - -0.8 2,727,794 + -0.6 2,727,794 + +0.4 2,727,794 + -1.5 2,727,794 + +0.9 2,727,794 + -0.0 2,727,794 + -1.0 2,727,794 + +1.9 2,727,794 + -1.1 2,727,794 + -2.4 2,727,794 + -1.6 2,727,794 + -2.7 2,727,794 + +0.1 2,727,794 + -0.4 2,727,794 + +0.0 2,727,794 + -1.4 2,727,795 - +1.9 2,727,795 - -1.9 2,727,795 - -2.3 2,727,795 - -2.2 2,727,795 - -1.0 2,727,795 - -2.3 2,727,795 - -1.7 2,727,795 - -0.6 2,727,795 - -1.2 2,727,795 - +1.9 2,727,795 - +0.2 2,727,795 - -0.9 2,727,795 - -1.2
Or see this region's nucleotide sequence