Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF84 and GFF85 are separated by 42 nucleotides GFF85 and GFF86 are separated by 324 nucleotides GFF86 and GFF87 are separated by 4 nucleotides GFF87 and GFF88 are separated by 375 nucleotides
GFF84 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 93,073 to 93,894
GFF84
GFF85 - 5-formyltetrahydrofolate cyclo-ligase, at 93,937 to 94,464
GFF85
GFF86 - hypothetical protein, at 94,789 to 95,172
GFF86
GFF87 - hypothetical protein, at 95,177 to 95,482
GFF87
GFF88 - Transketolase 1, at 95,858 to 97,864
GFF88
Position (kb)
94
95
96 Strain fitness (log2 ratio)
-1
0
1 at 94.047 kb on - strand, within GFF85 at 94.047 kb on - strand, within GFF85 at 94.100 kb on + strand, within GFF85 at 94.101 kb on - strand, within GFF85 at 94.464 kb on + strand at 94.623 kb on - strand at 94.629 kb on + strand at 94.642 kb on + strand at 94.642 kb on + strand at 94.642 kb on + strand at 94.643 kb on - strand at 94.745 kb on + strand at 94.746 kb on - strand at 95.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 94,047 - GFF85 0.21 -1.5 94,047 - GFF85 0.21 +1.2 94,100 + GFF85 0.31 -0.6 94,101 - GFF85 0.31 -0.4 94,464 + +0.1 94,623 - -1.6 94,629 + +0.2 94,642 + -0.9 94,642 + -0.8 94,642 + +0.6 94,643 - -0.4 94,745 + +0.5 94,746 - -0.8 95,813 + -0.1
Or see this region's nucleotide sequence