Strain Fitness in Xanthobacter sp. DMC5 around GFF853

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF851 and GFF852 are separated by 2 nucleotidesGFF852 and GFF853 are separated by 55 nucleotidesGFF853 and GFF854 are separated by 258 nucleotides GFF851 - Bifunctional enzyme IspD/IspF, at 917,866 to 919,065 GFF851 GFF852 - Nicotinamide-nucleotide amidohydrolase PncC, at 919,068 to 919,556 GFF852 GFF853 - hypothetical protein, at 919,612 to 921,342 GFF853 GFF854 - Arginine--tRNA ligase, at 921,601 to 923,361 GFF854 Position (kb) 919 920 921 922Strain fitness (log2 ratio) -2 -1 0 1 2at 919.083 kb on + strandat 919.231 kb on - strand, within GFF852at 921.575 kb on - strandat 921.575 kb on - strandat 921.575 kb on - strandat 921.575 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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919,083 + -1.8
919,231 - GFF852 0.33 -0.4
921,575 - -0.3
921,575 - +2.1
921,575 - -1.2
921,575 - -2.4

Or see this region's nucleotide sequence