Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF76 and GFF77 are separated by 156 nucleotides GFF77 and GFF78 are separated by 5 nucleotides GFF78 and GFF79 are separated by 10 nucleotides GFF79 and GFF80 are separated by 30 nucleotides
GFF76 - Holliday junction ATP-dependent DNA helicase RuvB, at 85,039 to 86,070
GFF76
GFF77 - hypothetical protein, at 86,227 to 86,769
GFF77
GFF78 - Holliday junction ATP-dependent DNA helicase RuvA, at 86,775 to 87,395
GFF78
GFF79 - hypothetical protein, at 87,406 to 87,837
GFF79
GFF80 - Crossover junction endodeoxyribonuclease RuvC, at 87,868 to 88,377
GFF80
Position (kb) 86
87
88 Strain fitness (log2 ratio) -1
0
1 at 86.115 kb on + strand at 86.172 kb on - strand at 86.172 kb on - strand at 86.202 kb on - strand at 86.267 kb on - strand at 86.267 kb on - strand at 86.268 kb on + strand at 86.269 kb on - strand at 87.320 kb on - strand, within GFF78 at 87.468 kb on + strand, within GFF79 at 87.468 kb on + strand, within GFF79 at 87.468 kb on + strand, within GFF79 at 87.469 kb on - strand, within GFF79 at 87.777 kb on + strand, within GFF79 at 87.777 kb on + strand, within GFF79 at 87.778 kb on - strand, within GFF79 at 87.778 kb on - strand, within GFF79 at 87.779 kb on + strand, within GFF79 at 87.779 kb on + strand, within GFF79 at 87.780 kb on - strand, within GFF79 at 87.780 kb on - strand, within GFF79 at 87.830 kb on + strand at 87.830 kb on + strand at 87.830 kb on + strand at 87.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 86,115 + +0.9 86,172 - -1.3 86,172 - -0.3 86,202 - +0.2 86,267 - +0.3 86,267 - -0.5 86,268 + -1.1 86,269 - -0.0 87,320 - GFF78 0.88 +0.4 87,468 + GFF79 0.14 -0.3 87,468 + GFF79 0.14 -0.3 87,468 + GFF79 0.14 +0.0 87,469 - GFF79 0.15 +0.9 87,777 + GFF79 0.86 +0.7 87,777 + GFF79 0.86 +0.2 87,778 - GFF79 0.86 +0.6 87,778 - GFF79 0.86 -0.0 87,779 + GFF79 0.86 +0.9 87,779 + GFF79 0.86 +0.2 87,780 - GFF79 0.87 -0.6 87,780 - GFF79 0.87 +0.3 87,830 + -0.7 87,830 + -0.1 87,830 + -0.6 87,869 + -0.2
Or see this region's nucleotide sequence