Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF74 and GFF75 are separated by 83 nucleotides GFF75 and GFF76 are separated by 45 nucleotides GFF76 and GFF77 are separated by 156 nucleotides GFF77 and GFF78 are separated by 5 nucleotides
GFF74 - hypothetical protein, at 83,870 to 84,277
GFF74
GFF75 - hypothetical protein, at 84,361 to 84,993
GFF75
GFF76 - Holliday junction ATP-dependent DNA helicase RuvB, at 85,039 to 86,070
GFF76
GFF77 - hypothetical protein, at 86,227 to 86,769
GFF77
GFF78 - Holliday junction ATP-dependent DNA helicase RuvA, at 86,775 to 87,395
GFF78
Position (kb) 85
86
87 Strain fitness (log2 ratio) -1
0
1 at 84.250 kb on - strand at 84.265 kb on - strand at 84.265 kb on - strand at 84.319 kb on - strand at 84.392 kb on + strand at 84.959 kb on + strand at 84.960 kb on - strand at 84.960 kb on - strand at 85.006 kb on + strand at 85.007 kb on - strand at 86.115 kb on + strand at 86.172 kb on - strand at 86.172 kb on - strand at 86.202 kb on - strand at 86.267 kb on - strand at 86.267 kb on - strand at 86.268 kb on + strand at 86.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 84,250 - -0.6 84,265 - +1.7 84,265 - -0.4 84,319 - -0.2 84,392 + +0.4 84,959 + +0.7 84,960 - +0.2 84,960 - +0.3 85,006 + +0.2 85,007 - -0.4 86,115 + +0.9 86,172 - -1.3 86,172 - -0.3 86,202 - +0.2 86,267 - +0.3 86,267 - -0.5 86,268 + -1.1 86,269 - -0.0
Or see this region's nucleotide sequence