Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF53 and GFF54 overlap by 1 nucleotides GFF54 and GFF55 overlap by 1 nucleotides GFF55 and GFF56 overlap by 4 nucleotides GFF56 and GFF57 are separated by 3 nucleotides GFF57 and GFF58 are separated by 74 nucleotides
GFF53 - hypothetical protein, at 64,885 to 65,463
GFF53
GFF54 - putative Nudix hydrolase NudL, at 65,463 to 66,140
GFF54
GFF55 - hypothetical protein, at 66,140 to 66,400
GFF55
GFF56 - CCA-adding enzyme, at 66,397 to 67,635
GFF56
GFF57 - hypothetical protein, at 67,639 to 67,848
GFF57
GFF58 - Oxygen-dependent coproporphyrinogen-III oxidase, at 67,923 to 68,870
GFF58
Position (kb) 66
67
68 Strain fitness (log2 ratio) -2
-1
0
1
2 at 66.347 kb on + strand, within GFF55 at 67.633 kb on + strand at 67.633 kb on + strand at 67.634 kb on - strand at 67.634 kb on - strand at 67.634 kb on - strand at 67.640 kb on + strand at 67.640 kb on + strand at 67.641 kb on - strand at 67.641 kb on - strand at 67.770 kb on - strand, within GFF57 at 67.899 kb on + strand at 67.900 kb on - strand at 67.910 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 66,347 + GFF55 0.79 +0.4 67,633 + -0.7 67,633 + +1.2 67,634 - -0.6 67,634 - -1.5 67,634 - +0.6 67,640 + +1.5 67,640 + +0.1 67,641 - +0.6 67,641 - -2.0 67,770 - GFF57 0.62 +2.2 67,899 + +0.4 67,900 - +0.3 67,910 + -0.4
Or see this region's nucleotide sequence