Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF52 and GFF53 are separated by 488 nucleotides GFF53 and GFF54 overlap by 1 nucleotides GFF54 and GFF55 overlap by 1 nucleotides GFF55 and GFF56 overlap by 4 nucleotides
GFF52 - hypothetical protein, at 63,395 to 64,396
GFF52
GFF53 - hypothetical protein, at 64,885 to 65,463
GFF53
GFF54 - putative Nudix hydrolase NudL, at 65,463 to 66,140
GFF54
GFF55 - hypothetical protein, at 66,140 to 66,400
GFF55
GFF56 - CCA-adding enzyme, at 66,397 to 67,635
GFF56
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1 at 64.397 kb on - strand at 64.397 kb on - strand at 64.397 kb on - strand at 64.397 kb on - strand at 64.410 kb on - strand at 64.452 kb on - strand at 64.689 kb on + strand at 64.690 kb on - strand at 64.690 kb on - strand at 64.690 kb on - strand at 64.694 kb on + strand at 64.694 kb on + strand at 64.996 kb on + strand, within GFF53 at 64.996 kb on + strand, within GFF53 at 64.996 kb on + strand, within GFF53 at 64.997 kb on - strand, within GFF53 at 64.997 kb on - strand, within GFF53 at 64.997 kb on - strand, within GFF53 at 64.997 kb on - strand, within GFF53 at 65.100 kb on - strand, within GFF53 at 65.100 kb on - strand, within GFF53 at 65.296 kb on + strand, within GFF53 at 66.347 kb on + strand, within GFF55
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 64,397 - +0.2 64,397 - -0.7 64,397 - -1.4 64,397 - -2.3 64,410 - -0.5 64,452 - -0.3 64,689 + +1.6 64,690 - +1.6 64,690 - +0.3 64,690 - -0.1 64,694 + +0.6 64,694 + -0.7 64,996 + GFF53 0.19 +0.9 64,996 + GFF53 0.19 -0.6 64,996 + GFF53 0.19 -2.2 64,997 - GFF53 0.19 -0.8 64,997 - GFF53 0.19 -1.2 64,997 - GFF53 0.19 -0.6 64,997 - GFF53 0.19 -0.1 65,100 - GFF53 0.37 +0.3 65,100 - GFF53 0.37 +0.8 65,296 + GFF53 0.71 -0.1 66,347 + GFF55 0.79 +0.4
Or see this region's nucleotide sequence