Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4800 and GFF4801 are separated by 9 nucleotides GFF4801 and GFF4802 are separated by 11 nucleotides GFF4802 and GFF4803 are separated by 1 nucleotides GFF4803 and GFF4804 are separated by 25 nucleotides
GFF4800 - 3-hydroxyisobutyrate dehydrogenase, at 40,079 to 40,957
GFF4800
GFF4801 - 2-(R)-hydroxypropyl-CoM dehydrogenase, at 40,967 to 41,719
GFF4801
GFF4802 - Metal reductase, at 41,731 to 43,764
GFF4802
GFF4803 - Short-chain-enoyl-CoA hydratase, at 43,766 to 44,578
GFF4803
GFF4804 - 3-oxoacyl-[acyl-carrier-protein] reductase FabG, at 44,604 to 45,383
GFF4804
Position (kb)
41
42
43
44 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 40.897 kb on + strand at 40.898 kb on - strand at 40.901 kb on + strand at 40.901 kb on + strand at 40.902 kb on - strand at 40.902 kb on - strand at 40.902 kb on - strand at 41.040 kb on + strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.041 kb on - strand at 41.051 kb on - strand, within GFF4801 at 41.051 kb on - strand, within GFF4801 at 41.129 kb on + strand, within GFF4801 at 41.129 kb on + strand, within GFF4801 at 41.130 kb on - strand, within GFF4801 at 41.130 kb on - strand, within GFF4801 at 41.199 kb on + strand, within GFF4801 at 41.199 kb on + strand, within GFF4801 at 41.200 kb on - strand, within GFF4801 at 41.200 kb on - strand, within GFF4801 at 41.200 kb on - strand, within GFF4801 at 41.200 kb on - strand, within GFF4801 at 41.200 kb on - strand, within GFF4801 at 41.253 kb on + strand, within GFF4801 at 41.254 kb on - strand, within GFF4801 at 41.254 kb on - strand, within GFF4801 at 41.254 kb on - strand, within GFF4801 at 41.254 kb on - strand, within GFF4801 at 41.370 kb on + strand, within GFF4801 at 41.643 kb on + strand, within GFF4801 at 41.643 kb on + strand, within GFF4801 at 41.643 kb on + strand, within GFF4801 at 41.643 kb on + strand, within GFF4801 at 41.643 kb on + strand, within GFF4801 at 41.644 kb on - strand at 41.668 kb on + strand at 41.724 kb on - strand at 41.724 kb on - strand at 41.724 kb on - strand at 41.724 kb on - strand at 41.724 kb on - strand at 41.772 kb on + strand at 41.772 kb on + strand at 41.773 kb on - strand at 42.252 kb on - strand, within GFF4802 at 42.257 kb on + strand, within GFF4802 at 42.257 kb on + strand, within GFF4802 at 42.258 kb on - strand, within GFF4802 at 42.621 kb on + strand, within GFF4802 at 42.621 kb on + strand, within GFF4802 at 42.622 kb on - strand, within GFF4802 at 42.680 kb on + strand, within GFF4802 at 42.680 kb on + strand, within GFF4802 at 42.680 kb on + strand, within GFF4802 at 42.680 kb on + strand, within GFF4802 at 42.681 kb on - strand, within GFF4802 at 42.681 kb on - strand, within GFF4802 at 42.682 kb on + strand, within GFF4802 at 42.769 kb on - strand, within GFF4802 at 42.816 kb on + strand, within GFF4802 at 42.817 kb on - strand, within GFF4802 at 42.817 kb on - strand, within GFF4802 at 42.817 kb on - strand, within GFF4802 at 42.838 kb on + strand, within GFF4802 at 42.839 kb on - strand, within GFF4802 at 42.839 kb on - strand, within GFF4802 at 42.906 kb on + strand, within GFF4802 at 42.906 kb on + strand, within GFF4802 at 42.906 kb on + strand, within GFF4802 at 42.907 kb on - strand, within GFF4802 at 42.930 kb on - strand, within GFF4802 at 42.963 kb on - strand, within GFF4802 at 42.963 kb on - strand, within GFF4802 at 42.963 kb on - strand, within GFF4802 at 43.196 kb on + strand, within GFF4802 at 43.197 kb on - strand, within GFF4802 at 43.277 kb on + strand, within GFF4802 at 43.278 kb on - strand, within GFF4802 at 43.278 kb on - strand, within GFF4802 at 43.368 kb on - strand, within GFF4802 at 43.368 kb on - strand, within GFF4802 at 43.466 kb on + strand, within GFF4802 at 43.466 kb on + strand, within GFF4802 at 43.626 kb on - strand at 43.695 kb on + strand at 43.696 kb on - strand at 43.748 kb on + strand at 43.748 kb on + strand at 43.748 kb on + strand at 43.749 kb on - strand at 43.820 kb on + strand at 43.964 kb on + strand, within GFF4803 at 43.964 kb on + strand, within GFF4803 at 43.964 kb on + strand, within GFF4803 at 43.965 kb on - strand, within GFF4803 at 43.965 kb on - strand, within GFF4803 at 44.076 kb on + strand, within GFF4803 at 44.076 kb on + strand, within GFF4803 at 44.077 kb on - strand, within GFF4803 at 44.077 kb on - strand, within GFF4803 at 44.283 kb on + strand, within GFF4803 at 44.283 kb on + strand, within GFF4803 at 44.284 kb on - strand, within GFF4803 at 44.348 kb on + strand, within GFF4803 at 44.348 kb on + strand, within GFF4803 at 44.349 kb on - strand, within GFF4803 at 44.349 kb on - strand, within GFF4803 at 44.447 kb on + strand, within GFF4803 at 44.447 kb on + strand, within GFF4803 at 44.447 kb on + strand, within GFF4803 at 44.447 kb on + strand, within GFF4803 at 44.447 kb on + strand, within GFF4803 at 44.448 kb on - strand, within GFF4803 at 44.448 kb on - strand, within GFF4803 at 44.448 kb on - strand, within GFF4803 at 44.562 kb on + strand at 44.563 kb on - strand at 44.563 kb on - strand at 44.662 kb on + strand at 44.662 kb on + strand at 44.663 kb on - strand at 44.663 kb on - strand at 44.738 kb on - strand, within GFF4804 at 44.756 kb on + strand, within GFF4804 at 44.756 kb on + strand, within GFF4804 at 44.757 kb on - strand, within GFF4804 at 44.758 kb on + strand, within GFF4804 at 44.759 kb on - strand, within GFF4804 at 44.759 kb on - strand, within GFF4804 at 44.759 kb on - strand, within GFF4804
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 40,897 + -0.3 40,898 - -0.6 40,901 + -1.2 40,901 + -0.8 40,902 - -0.1 40,902 - -0.4 40,902 - +2.3 41,040 + -0.6 41,041 - +0.9 41,041 - -0.2 41,041 - -0.8 41,041 - +1.0 41,041 - -1.1 41,041 - -0.7 41,041 - +1.0 41,041 - +0.7 41,051 - GFF4801 0.11 -1.1 41,051 - GFF4801 0.11 -1.7 41,129 + GFF4801 0.22 +1.3 41,129 + GFF4801 0.22 +0.9 41,130 - GFF4801 0.22 -1.3 41,130 - GFF4801 0.22 +1.3 41,199 + GFF4801 0.31 +0.9 41,199 + GFF4801 0.31 +0.6 41,200 - GFF4801 0.31 -0.1 41,200 - GFF4801 0.31 -0.4 41,200 - GFF4801 0.31 -1.4 41,200 - GFF4801 0.31 +1.7 41,200 - GFF4801 0.31 -1.1 41,253 + GFF4801 0.38 +0.7 41,254 - GFF4801 0.38 -0.6 41,254 - GFF4801 0.38 -0.8 41,254 - GFF4801 0.38 +0.9 41,254 - GFF4801 0.38 +0.4 41,370 + GFF4801 0.54 +1.1 41,643 + GFF4801 0.90 +2.0 41,643 + GFF4801 0.90 +0.5 41,643 + GFF4801 0.90 +0.5 41,643 + GFF4801 0.90 -0.5 41,643 + GFF4801 0.90 +0.5 41,644 - -0.2 41,668 + +0.0 41,724 - +0.3 41,724 - +0.7 41,724 - -0.9 41,724 - -1.1 41,724 - +1.3 41,772 + +0.5 41,772 + -1.3 41,773 - -0.6 42,252 - GFF4802 0.26 -0.2 42,257 + GFF4802 0.26 +0.5 42,257 + GFF4802 0.26 -1.0 42,258 - GFF4802 0.26 -0.7 42,621 + GFF4802 0.44 -1.4 42,621 + GFF4802 0.44 -1.1 42,622 - GFF4802 0.44 +0.4 42,680 + GFF4802 0.47 +1.4 42,680 + GFF4802 0.47 -2.0 42,680 + GFF4802 0.47 +0.2 42,680 + GFF4802 0.47 +0.5 42,681 - GFF4802 0.47 -1.4 42,681 - GFF4802 0.47 -0.6 42,682 + GFF4802 0.47 +0.5 42,769 - GFF4802 0.51 -0.4 42,816 + GFF4802 0.53 -0.1 42,817 - GFF4802 0.53 +0.5 42,817 - GFF4802 0.53 -0.5 42,817 - GFF4802 0.53 -1.0 42,838 + GFF4802 0.54 +0.4 42,839 - GFF4802 0.54 -1.1 42,839 - GFF4802 0.54 -0.7 42,906 + GFF4802 0.58 -0.1 42,906 + GFF4802 0.58 -0.3 42,906 + GFF4802 0.58 -1.8 42,907 - GFF4802 0.58 +1.2 42,930 - GFF4802 0.59 -1.1 42,963 - GFF4802 0.61 -1.0 42,963 - GFF4802 0.61 -0.6 42,963 - GFF4802 0.61 -0.0 43,196 + GFF4802 0.72 -0.3 43,197 - GFF4802 0.72 +0.9 43,277 + GFF4802 0.76 +0.4 43,278 - GFF4802 0.76 +0.4 43,278 - GFF4802 0.76 +1.3 43,368 - GFF4802 0.80 +0.1 43,368 - GFF4802 0.80 +0.3 43,466 + GFF4802 0.85 -2.3 43,466 + GFF4802 0.85 +0.4 43,626 - +0.7 43,695 + -0.2 43,696 - -0.4 43,748 + +0.4 43,748 + -0.5 43,748 + +0.7 43,749 - +0.5 43,820 + +0.6 43,964 + GFF4803 0.24 +0.4 43,964 + GFF4803 0.24 +0.7 43,964 + GFF4803 0.24 -0.4 43,965 - GFF4803 0.24 +0.6 43,965 - GFF4803 0.24 -0.4 44,076 + GFF4803 0.38 -0.3 44,076 + GFF4803 0.38 -1.8 44,077 - GFF4803 0.38 -0.9 44,077 - GFF4803 0.38 +0.8 44,283 + GFF4803 0.64 +0.7 44,283 + GFF4803 0.64 -0.8 44,284 - GFF4803 0.64 +0.9 44,348 + GFF4803 0.72 -1.5 44,348 + GFF4803 0.72 -0.1 44,349 - GFF4803 0.72 -0.1 44,349 - GFF4803 0.72 +0.9 44,447 + GFF4803 0.84 -1.0 44,447 + GFF4803 0.84 +0.7 44,447 + GFF4803 0.84 -1.4 44,447 + GFF4803 0.84 +0.6 44,447 + GFF4803 0.84 -0.7 44,448 - GFF4803 0.84 -1.5 44,448 - GFF4803 0.84 +2.0 44,448 - GFF4803 0.84 -1.3 44,562 + +0.5 44,563 - -0.3 44,563 - +0.3 44,662 + -0.3 44,662 + +0.9 44,663 - -0.1 44,663 - +0.5 44,738 - GFF4804 0.17 -0.1 44,756 + GFF4804 0.19 -0.5 44,756 + GFF4804 0.19 -0.4 44,757 - GFF4804 0.20 -0.5 44,758 + GFF4804 0.20 -1.2 44,759 - GFF4804 0.20 +0.1 44,759 - GFF4804 0.20 -0.0 44,759 - GFF4804 0.20 -0.3
Or see this region's nucleotide sequence