Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4576 and GFF4577 are separated by 2 nucleotides GFF4577 and GFF4578 are separated by 224 nucleotides GFF4578 and GFF4579 overlap by 34 nucleotides GFF4579 and GFF4580 are separated by 211 nucleotides GFF4580 and GFF4581 are separated by 74 nucleotides
GFF4576 - hypothetical protein, at 164,711 to 165,289
GFF4576
GFF4577 - Chromosomal replication initiator protein DnaA, at 165,292 to 165,675
GFF4577
GFF4578 - hypothetical protein, at 165,900 to 166,199
GFF4578
GFF4579 - O-acetylserine sulfhydrylase, at 166,166 to 167,149
GFF4579
GFF4580 - HTH-type transcriptional regulator CymR, at 167,361 to 167,801
GFF4580
GFF4581 - Deoxyuridine 5'-triphosphate nucleotidohydrolase, at 167,876 to 168,340
GFF4581
Position (kb)
166
167
168 Strain fitness (log2 ratio)
-2
-1
0
1 at 165.467 kb on + strand, within GFF4577 at 165.467 kb on + strand, within GFF4577 at 165.900 kb on - strand at 166.071 kb on + strand, within GFF4578 at 166.116 kb on - strand, within GFF4578 at 166.197 kb on + strand at 167.088 kb on + strand at 167.089 kb on - strand at 167.288 kb on + strand at 167.289 kb on - strand at 167.289 kb on - strand at 167.820 kb on + strand at 167.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 165,467 + GFF4577 0.46 +1.0 165,467 + GFF4577 0.46 +1.2 165,900 - -0.5 166,071 + GFF4578 0.57 +0.4 166,116 - GFF4578 0.72 +0.3 166,197 + +0.6 167,088 + +0.3 167,089 - -0.4 167,288 + -2.0 167,289 - +0.2 167,289 - -0.2 167,820 + -1.8 167,821 - -0.8
Or see this region's nucleotide sequence