Strain Fitness in Xanthobacter sp. DMC5 around GFF4332

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4331 and GFF4332 overlap by 4 nucleotidesGFF4332 and GFF4333 are separated by 14 nucleotidesGFF4333 and GFF4334 overlap by 4 nucleotides GFF4331 - putative acyl--CoA ligase YhfT, at 114,346 to 115,806 GFF4331 GFF4332 - Biotin transporter BioY, at 115,803 to 116,378 GFF4332 GFF4333 - Energy-coupling factor transporter transmembrane protein BioN, at 116,393 to 117,025 GFF4333 GFF4334 - Biotin transport ATP-binding protein BioM, at 117,022 to 117,792 GFF4334 Position (kb) 115 116 117Strain fitness (log2 ratio) -1 0 1 2at 114.960 kb on - strand, within GFF4331at 115.121 kb on + strand, within GFF4331at 115.122 kb on - strand, within GFF4331at 115.229 kb on + strand, within GFF4331at 115.229 kb on + strand, within GFF4331at 115.229 kb on + strand, within GFF4331at 115.230 kb on - strand, within GFF4331at 115.230 kb on - strand, within GFF4331at 115.388 kb on + strand, within GFF4331at 115.388 kb on + strand, within GFF4331at 115.388 kb on + strand, within GFF4331at 115.389 kb on - strand, within GFF4331at 115.389 kb on - strand, within GFF4331at 115.721 kb on + strandat 115.721 kb on + strandat 117.013 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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114,960 - GFF4331 0.42 +1.3
115,121 + GFF4331 0.53 +0.0
115,122 - GFF4331 0.53 -0.4
115,229 + GFF4331 0.60 +0.2
115,229 + GFF4331 0.60 +0.4
115,229 + GFF4331 0.60 -1.7
115,230 - GFF4331 0.61 -1.7
115,230 - GFF4331 0.61 -0.7
115,388 + GFF4331 0.71 -0.8
115,388 + GFF4331 0.71 +2.0
115,388 + GFF4331 0.71 +1.2
115,389 - GFF4331 0.71 +0.3
115,389 - GFF4331 0.71 +0.4
115,721 + -0.6
115,721 + -1.0
117,013 - -1.7

Or see this region's nucleotide sequence