Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4180 and GFF4181 are separated by 233 nucleotides GFF4181 and GFF4182 are separated by 255 nucleotides GFF4182 and GFF4183 are separated by 89 nucleotides
GFF4180 - L-2-hydroxyglutarate dehydrogenase, at 194,879 to 195,982
GFF4180
GFF4181 - GTP-binding protein TypA/BipA, at 196,216 to 198,045
GFF4181
GFF4182 - hypothetical protein, at 198,301 to 198,738
GFF4182
GFF4183 - hypothetical protein, at 198,828 to 200,210
GFF4183
Position (kb) 196
197
198
199 Strain fitness (log2 ratio) -1
0
1 at 195.572 kb on + strand, within GFF4180 at 195.573 kb on - strand, within GFF4180 at 195.716 kb on + strand, within GFF4180 at 195.717 kb on - strand, within GFF4180 at 195.717 kb on - strand, within GFF4180 at 196.047 kb on + strand at 198.103 kb on - strand at 198.392 kb on + strand, within GFF4182 at 198.407 kb on - strand, within GFF4182 at 198.527 kb on - strand, within GFF4182 at 198.563 kb on - strand, within GFF4182 at 198.563 kb on - strand, within GFF4182 at 198.566 kb on - strand, within GFF4182 at 198.736 kb on + strand at 198.769 kb on + strand at 198.770 kb on - strand at 198.770 kb on - strand at 198.794 kb on + strand at 198.794 kb on + strand at 198.795 kb on - strand at 198.796 kb on + strand at 198.797 kb on - strand at 198.797 kb on - strand at 198.797 kb on - strand at 198.797 kb on - strand at 198.930 kb on + strand at 198.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 195,572 + GFF4180 0.63 +0.7 195,573 - GFF4180 0.63 +0.5 195,716 + GFF4180 0.76 -1.1 195,717 - GFF4180 0.76 -0.3 195,717 - GFF4180 0.76 +0.6 196,047 + -0.1 198,103 - -0.2 198,392 + GFF4182 0.21 -0.2 198,407 - GFF4182 0.24 +1.2 198,527 - GFF4182 0.52 -0.2 198,563 - GFF4182 0.60 +0.7 198,563 - GFF4182 0.60 +0.0 198,566 - GFF4182 0.61 +0.4 198,736 + +1.0 198,769 + -0.2 198,770 - -1.0 198,770 - -0.2 198,794 + -0.5 198,794 + -1.3 198,795 - -0.7 198,796 + -0.4 198,797 - -0.3 198,797 - +0.0 198,797 - -1.3 198,797 - -0.0 198,930 + +0.2 198,930 + -0.3
Or see this region's nucleotide sequence