Strain Fitness in Xanthobacter sp. DMC5 around GFF4146

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4144 and GFF4145 overlap by 4 nucleotidesGFF4145 and GFF4146 are separated by 15 nucleotidesGFF4146 and GFF4147 are separated by 88 nucleotides GFF4144 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 152,511 to 153,575 GFF4144 GFF4145 - Ribosomal RNA small subunit methyltransferase A, at 153,572 to 154,450 GFF4145 GFF4146 - Methionine synthase, at 154,466 to 158,221 GFF4146 GFF4147 - 5,10-methylenetetrahydrofolate reductase, at 158,310 to 159,251 GFF4147 Position (kb) 154 155 156 157 158 159Strain fitness (log2 ratio) -2 -1 0 1at 153.848 kb on + strand, within GFF4145at 153.848 kb on + strand, within GFF4145at 153.848 kb on + strand, within GFF4145at 153.947 kb on + strand, within GFF4145at 153.947 kb on + strand, within GFF4145at 154.080 kb on - strand, within GFF4145at 154.448 kb on + strandat 154.449 kb on - strandat 154.449 kb on - strandat 154.467 kb on + strandat 154.468 kb on - strandat 154.498 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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153,848 + GFF4145 0.31 +0.4
153,848 + GFF4145 0.31 -0.4
153,848 + GFF4145 0.31 +0.4
153,947 + GFF4145 0.43 +1.0
153,947 + GFF4145 0.43 +0.4
154,080 - GFF4145 0.58 -2.0
154,448 + +1.2
154,449 - -0.7
154,449 - -1.1
154,467 + +0.8
154,468 - +0.4
154,498 - +0.2

Or see this region's nucleotide sequence