Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF33 and GFF34 are separated by 62 nucleotides GFF34 and GFF35 overlap by 4 nucleotides
GFF33 - Beta-ketothiolase BktB, at 40,369 to 41,553
GFF33
GFF34 - (2R)-3-sulfolactate dehydrogenase (NADP(+)), at 41,616 to 42,647
GFF34
GFF35 - Acetophenone carboxylase delta subunit, at 42,644 to 44,338
GFF35
Position (kb) 41
42
43 Strain fitness (log2 ratio) -2
-1
0
1 at 40.744 kb on + strand, within GFF33 at 40.745 kb on - strand, within GFF33 at 40.955 kb on - strand, within GFF33 at 40.955 kb on - strand, within GFF33 at 41.489 kb on - strand at 41.565 kb on + strand at 41.590 kb on - strand at 42.278 kb on - strand, within GFF34 at 42.466 kb on + strand, within GFF34 at 42.467 kb on - strand, within GFF34 at 42.679 kb on - strand at 42.679 kb on - strand at 42.679 kb on - strand at 42.685 kb on - strand at 42.826 kb on + strand, within GFF35 at 42.922 kb on - strand, within GFF35 at 43.072 kb on - strand, within GFF35 at 43.230 kb on + strand, within GFF35 at 43.230 kb on + strand, within GFF35 at 43.231 kb on - strand, within GFF35 at 43.236 kb on + strand, within GFF35 at 43.237 kb on - strand, within GFF35 at 43.237 kb on - strand, within GFF35 at 43.248 kb on + strand, within GFF35 at 43.248 kb on + strand, within GFF35 at 43.248 kb on + strand, within GFF35 at 43.251 kb on + strand, within GFF35 at 43.252 kb on - strand, within GFF35 at 43.458 kb on + strand, within GFF35 at 43.458 kb on + strand, within GFF35 at 43.459 kb on - strand, within GFF35
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 40,744 + GFF33 0.32 -1.4 40,745 - GFF33 0.32 -0.2 40,955 - GFF33 0.49 -0.6 40,955 - GFF33 0.49 -1.8 41,489 - +0.5 41,565 + -1.4 41,590 - -0.7 42,278 - GFF34 0.64 -0.4 42,466 + GFF34 0.82 -0.9 42,467 - GFF34 0.82 +1.0 42,679 - -0.6 42,679 - -1.8 42,679 - -0.4 42,685 - -0.6 42,826 + GFF35 0.11 +1.2 42,922 - GFF35 0.16 -0.7 43,072 - GFF35 0.25 +1.6 43,230 + GFF35 0.35 -0.1 43,230 + GFF35 0.35 -0.5 43,231 - GFF35 0.35 +0.4 43,236 + GFF35 0.35 -1.0 43,237 - GFF35 0.35 +0.9 43,237 - GFF35 0.35 -0.4 43,248 + GFF35 0.36 -0.8 43,248 + GFF35 0.36 +1.4 43,248 + GFF35 0.36 +0.6 43,251 + GFF35 0.36 -0.2 43,252 - GFF35 0.36 -0.7 43,458 + GFF35 0.48 +0.1 43,458 + GFF35 0.48 +1.2 43,459 - GFF35 0.48 +1.0
Or see this region's nucleotide sequence