Strain Fitness in Xanthobacter sp. DMC5 around GFF3280

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3277 and GFF3278 are separated by 139 nucleotidesGFF3278 and GFF3279 are separated by 199 nucleotidesGFF3279 and GFF3280 are separated by 72 nucleotidesGFF3280 and GFF3281 are separated by 47 nucleotides GFF3277 - Glutarate-semialdehyde dehydrogenase, at 111,486 to 112,964 GFF3277 GFF3278 - hypothetical protein, at 113,104 to 113,325 GFF3278 GFF3279 - hypothetical protein, at 113,525 to 113,731 GFF3279 GFF3280 - Hydrogenobyrinate a,c-diamide synthase, at 113,804 to 115,138 GFF3280 GFF3281 - Cobalt-precorrin-5B C(1)-methyltransferase, at 115,186 to 116,319 GFF3281 Position (kb) 113 114 115 116Strain fitness (log2 ratio) -1 0 1at 113.052 kb on + strandat 113.052 kb on + strandat 113.053 kb on - strandat 113.085 kb on - strandat 113.183 kb on + strand, within GFF3278at 113.183 kb on + strand, within GFF3278at 113.183 kb on + strand, within GFF3278at 113.184 kb on - strand, within GFF3278at 113.390 kb on + strandat 113.391 kb on - strandat 113.391 kb on - strandat 113.392 kb on + strandat 113.491 kb on + strandat 113.491 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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113,052 + -0.7
113,052 + -0.6
113,053 - +1.3
113,085 - -0.2
113,183 + GFF3278 0.36 -0.8
113,183 + GFF3278 0.36 +0.4
113,183 + GFF3278 0.36 +1.0
113,184 - GFF3278 0.36 -0.5
113,390 + +0.3
113,391 - -0.0
113,391 - +0.1
113,392 + -0.6
113,491 + +0.2
113,491 + +0.2

Or see this region's nucleotide sequence