Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3231 and GFF3232 are separated by 8 nucleotides GFF3232 and GFF3233 are separated by 16 nucleotides GFF3233 and GFF3234 are separated by 229 nucleotides GFF3234 and GFF3235 are separated by 79 nucleotides
GFF3231 - Glucose-6-phosphate 1-dehydrogenase, at 56,339 to 57,814
GFF3231
GFF3232 - 6-phosphogluconolactonase, at 57,823 to 58,533
GFF3232
GFF3233 - Glucose-6-phosphate isomerase, at 58,550 to 60,196
GFF3233
GFF3234 - hypothetical protein, at 60,426 to 60,680
GFF3234
GFF3235 - Oxalate:formate antiporter, at 60,760 to 62,067
GFF3235
Position (kb)
58
59
60
61 Strain fitness (log2 ratio)
-1
0
1 at 57.610 kb on + strand, within GFF3231 at 57.610 kb on + strand, within GFF3231 at 57.610 kb on + strand, within GFF3231 at 57.610 kb on + strand, within GFF3231 at 57.611 kb on - strand, within GFF3231 at 57.611 kb on - strand, within GFF3231 at 57.611 kb on - strand, within GFF3231 at 57.611 kb on - strand, within GFF3231 at 57.734 kb on + strand at 58.144 kb on + strand, within GFF3232 at 58.145 kb on - strand, within GFF3232 at 58.254 kb on + strand, within GFF3232 at 60.268 kb on - strand at 60.341 kb on + strand at 60.342 kb on - strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.426 kb on - strand at 60.426 kb on - strand at 60.426 kb on - strand at 60.426 kb on - strand at 60.444 kb on - strand at 60.444 kb on - strand at 60.444 kb on - strand at 60.510 kb on + strand, within GFF3234 at 60.605 kb on + strand, within GFF3234 at 60.731 kb on + strand at 60.977 kb on + strand, within GFF3235 at 61.049 kb on + strand, within GFF3235 at 61.049 kb on + strand, within GFF3235 at 61.049 kb on + strand, within GFF3235
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 57,610 + GFF3231 0.86 -0.1 57,610 + GFF3231 0.86 -0.5 57,610 + GFF3231 0.86 +0.9 57,610 + GFF3231 0.86 -0.8 57,611 - GFF3231 0.86 -0.1 57,611 - GFF3231 0.86 -1.7 57,611 - GFF3231 0.86 +0.3 57,611 - GFF3231 0.86 -0.9 57,734 + +1.0 58,144 + GFF3232 0.45 -0.2 58,145 - GFF3232 0.45 -0.5 58,254 + GFF3232 0.61 +0.6 60,268 - +0.6 60,341 + -0.4 60,342 - -0.2 60,425 + -0.0 60,425 + -0.7 60,425 + -0.9 60,425 + -1.0 60,425 + -1.1 60,425 + -0.0 60,426 - -0.3 60,426 - -0.5 60,426 - +1.7 60,426 - -0.2 60,444 - -0.5 60,444 - +0.3 60,444 - -0.0 60,510 + GFF3234 0.33 +1.2 60,605 + GFF3234 0.70 +0.7 60,731 + +1.3 60,977 + GFF3235 0.17 -0.7 61,049 + GFF3235 0.22 -0.5 61,049 + GFF3235 0.22 -0.9 61,049 + GFF3235 0.22 -1.4
Or see this region's nucleotide sequence