Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3083 and GFF3084 are separated by 32 nucleotides GFF3084 and GFF3085 are separated by 91 nucleotides GFF3085 and GFF3086 are separated by 88 nucleotides GFF3086 and GFF3087 are separated by 258 nucleotides
GFF3083 - hypothetical protein, at 813,373 to 814,317
GFF3083
GFF3084 - hypothetical protein, at 814,350 to 814,685
GFF3084
GFF3085 - hypothetical protein, at 814,777 to 815,013
GFF3085
GFF3086 - hypothetical protein, at 815,102 to 815,395
GFF3086
GFF3087 - Threonine--tRNA ligase, at 815,654 to 817,630
GFF3087
Position (kb) 814
815 Strain fitness (log2 ratio) -2
-1
0
1
2 at 814.683 kb on + strand at 814.683 kb on + strand at 814.684 kb on - strand at 814.896 kb on - strand, within GFF3085 at 815.044 kb on + strand at 815.287 kb on - strand, within GFF3086 at 815.328 kb on - strand, within GFF3086 at 815.427 kb on - strand at 815.431 kb on - strand at 815.542 kb on - strand at 815.542 kb on - strand at 815.584 kb on - strand at 815.598 kb on + strand at 815.598 kb on + strand at 815.607 kb on - strand at 815.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 814,683 + -0.2 814,683 + +0.6 814,684 - +0.1 814,896 - GFF3085 0.50 +0.4 815,044 + -0.9 815,287 - GFF3086 0.63 +0.8 815,328 - GFF3086 0.77 -0.8 815,427 - +0.5 815,431 - +0.6 815,542 - -0.6 815,542 - -1.1 815,584 - -1.7 815,598 + +2.0 815,598 + +0.1 815,607 - -1.8 815,627 + +1.0
Or see this region's nucleotide sequence