Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3078 and GFF3079 are separated by 435 nucleotides GFF3079 and GFF3080 are separated by 188 nucleotides GFF3080 and GFF3081 overlap by 4 nucleotides
GFF3078 - ECF RNA polymerase sigma factor RpoE, at 809,448 to 810,029
GFF3078
GFF3079 - 5,6,7,8-tetrahydromethanopterin hydro-lyase, at 810,465 to 811,004
GFF3079
GFF3080 - hypothetical protein, at 811,193 to 811,336
GFF3080
GFF3081 - Tyrosine recombinase XerD, at 811,333 to 812,292
GFF3081
Position (kb) 810
811
812 Strain fitness (log2 ratio) -1
0
1
2 at 809.707 kb on + strand, within GFF3078 at 809.708 kb on - strand, within GFF3078 at 809.711 kb on + strand, within GFF3078 at 809.711 kb on + strand, within GFF3078 at 809.712 kb on - strand, within GFF3078 at 809.807 kb on - strand, within GFF3078 at 809.891 kb on - strand, within GFF3078 at 809.891 kb on - strand, within GFF3078 at 810.494 kb on - strand at 810.583 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 810.766 kb on + strand, within GFF3079 at 811.247 kb on + strand, within GFF3080 at 811.820 kb on - strand, within GFF3081 at 811.850 kb on + strand, within GFF3081 at 811.851 kb on - strand, within GFF3081
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 809,707 + GFF3078 0.45 +0.2 809,708 - GFF3078 0.45 -0.2 809,711 + GFF3078 0.45 +0.3 809,711 + GFF3078 0.45 -0.6 809,712 - GFF3078 0.45 -0.8 809,807 - GFF3078 0.62 -0.9 809,891 - GFF3078 0.76 +1.2 809,891 - GFF3078 0.76 -0.1 810,494 - -1.1 810,583 + GFF3079 0.22 +1.4 810,766 + GFF3079 0.56 -1.0 810,766 + GFF3079 0.56 -0.6 810,766 + GFF3079 0.56 +1.6 810,766 + GFF3079 0.56 +0.9 811,247 + GFF3080 0.38 +1.6 811,820 - GFF3081 0.51 +1.4 811,850 + GFF3081 0.54 +2.0 811,851 - GFF3081 0.54 +1.4
Or see this region's nucleotide sequence