Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3009 and GFF3010 are separated by 162 nucleotides GFF3010 and GFF3011 are separated by 9 nucleotides GFF3011 and GFF3012 overlap by 1 nucleotides
GFF3009 - 1-deoxyxylulose-5-phosphate synthase YajO, at 734,885 to 735,859
GFF3009
GFF3010 - hypothetical protein, at 736,022 to 736,180
GFF3010
GFF3011 - Pimeloyl-[acyl-carrier protein] methyl ester esterase, at 736,190 to 737,011
GFF3011
GFF3012 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 737,011 to 737,727
GFF3012
Position (kb)
736
737 Strain fitness (log2 ratio)
-1
0
1
2 at 735.066 kb on - strand, within GFF3009 at 735.248 kb on + strand, within GFF3009 at 735.248 kb on + strand, within GFF3009 at 735.249 kb on - strand, within GFF3009 at 735.249 kb on - strand, within GFF3009 at 735.260 kb on + strand, within GFF3009 at 735.260 kb on + strand, within GFF3009 at 735.396 kb on - strand, within GFF3009 at 735.514 kb on + strand, within GFF3009 at 735.608 kb on + strand, within GFF3009 at 735.824 kb on + strand at 735.824 kb on + strand at 735.824 kb on + strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 735,066 - GFF3009 0.19 +0.0 735,248 + GFF3009 0.37 -1.2 735,248 + GFF3009 0.37 +0.8 735,249 - GFF3009 0.37 +0.8 735,249 - GFF3009 0.37 -0.6 735,260 + GFF3009 0.38 +0.3 735,260 + GFF3009 0.38 +1.8 735,396 - GFF3009 0.52 +0.8 735,514 + GFF3009 0.65 -0.1 735,608 + GFF3009 0.74 -1.6 735,824 + +0.2 735,824 + +0.6 735,824 + -0.4 735,825 - +0.4 735,825 - +0.1 735,825 - -0.6 735,825 - -0.8 735,825 - +0.5 735,825 - -0.1
Or see this region's nucleotide sequence