Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF26 and GFF27 overlap by 34 nucleotides GFF27 and GFF28 are separated by 54 nucleotides GFF28 and GFF29 are separated by 141 nucleotides
GFF26 - Acetyl-CoA:oxalate CoA-transferase, at 31,106 to 32,308
GFF26
GFF27 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 32,275 to 33,018
GFF27
GFF28 - HTH-type transcriptional repressor RspR, at 33,073 to 33,801
GFF28
GFF29 - 2,3-diketo-L-gulonate-binding periplasmic protein YiaO, at 33,943 to 34,971
GFF29
Position (kb) 33
34 Strain fitness (log2 ratio) -1
0
1 at 32.183 kb on + strand, within GFF26 at 32.184 kb on + strand, within GFF26 at 32.185 kb on - strand, within GFF26 at 32.642 kb on - strand, within GFF27 at 32.642 kb on - strand, within GFF27 at 32.995 kb on + strand at 32.996 kb on - strand at 33.033 kb on + strand at 33.033 kb on + strand at 33.034 kb on - strand at 33.826 kb on - strand at 33.826 kb on - strand at 33.826 kb on - strand at 34.312 kb on + strand, within GFF29 at 34.312 kb on + strand, within GFF29 at 34.312 kb on + strand, within GFF29 at 34.313 kb on - strand, within GFF29 at 34.565 kb on - strand, within GFF29 at 34.621 kb on + strand, within GFF29 at 34.621 kb on + strand, within GFF29 at 34.622 kb on - strand, within GFF29 at 34.622 kb on - strand, within GFF29 at 34.652 kb on - strand, within GFF29
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 32,183 + GFF26 0.90 +1.0 32,184 + GFF26 0.90 -0.1 32,185 - GFF26 0.90 -0.1 32,642 - GFF27 0.49 +0.1 32,642 - GFF27 0.49 -0.8 32,995 + +0.7 32,996 - +0.4 33,033 + -0.7 33,033 + -0.0 33,034 - -0.3 33,826 - +0.2 33,826 - +0.4 33,826 - -0.8 34,312 + GFF29 0.36 +0.8 34,312 + GFF29 0.36 +0.3 34,312 + GFF29 0.36 +1.2 34,313 - GFF29 0.36 -0.1 34,565 - GFF29 0.60 -1.2 34,621 + GFF29 0.66 -0.3 34,621 + GFF29 0.66 -0.6 34,622 - GFF29 0.66 -1.7 34,622 - GFF29 0.66 -0.2 34,652 - GFF29 0.69 -1.3
Or see this region's nucleotide sequence