Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF252 and GFF253 are separated by 195 nucleotides GFF253 and GFF254 are separated by 42 nucleotides GFF254 and GFF255 are separated by 291 nucleotides GFF255 and GFF256 are separated by 52 nucleotides
GFF252 - hypothetical protein, at 266,020 to 267,132
GFF252
GFF253 - 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxonon atrienedioate hydrolase, at 267,328 to 267,927
GFF253
GFF254 - Thymidylate synthase, at 267,970 to 268,764
GFF254
GFF255 - hypothetical protein, at 269,056 to 269,337
GFF255
GFF256 - Biotin synthase, at 269,390 to 270,361
GFF256
Position (kb) 267
268
269 Strain fitness (log2 ratio) -1
0
1 at 267.190 kb on + strand at 267.191 kb on - strand at 267.199 kb on - strand at 267.199 kb on - strand at 267.199 kb on - strand at 267.201 kb on + strand at 267.201 kb on + strand at 267.201 kb on + strand at 267.202 kb on - strand at 267.230 kb on + strand at 267.231 kb on - strand at 267.271 kb on - strand at 267.271 kb on - strand at 267.329 kb on + strand at 267.330 kb on - strand at 267.593 kb on + strand, within GFF253 at 267.711 kb on - strand, within GFF253 at 267.711 kb on - strand, within GFF253 at 267.972 kb on - strand at 268.770 kb on - strand at 268.967 kb on + strand at 268.968 kb on - strand at 269.011 kb on + strand at 269.011 kb on + strand at 269.012 kb on - strand at 269.012 kb on - strand at 269.104 kb on + strand, within GFF255 at 269.473 kb on + strand at 269.473 kb on + strand at 269.474 kb on - strand at 269.481 kb on + strand at 269.481 kb on + strand at 269.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 267,190 + +0.3 267,191 - +0.2 267,199 - -0.0 267,199 - -0.7 267,199 - -0.7 267,201 + -0.2 267,201 + +1.0 267,201 + +0.6 267,202 - +0.1 267,230 + -0.8 267,231 - -0.3 267,271 - -0.6 267,271 - -0.3 267,329 + +0.7 267,330 - +0.1 267,593 + GFF253 0.44 -0.1 267,711 - GFF253 0.64 -0.7 267,711 - GFF253 0.64 -0.6 267,972 - -0.5 268,770 - +0.3 268,967 + -1.0 268,968 - -0.6 269,011 + -0.2 269,011 + +1.4 269,012 - -0.5 269,012 - -0.6 269,104 + GFF255 0.17 -0.2 269,473 + -0.2 269,473 + +0.7 269,474 - -0.5 269,481 + +0.2 269,481 + -0.9 269,482 - -0.5
Or see this region's nucleotide sequence