Strain Fitness in Xanthobacter sp. DMC5 around GFF2

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1 and GFF2 are separated by 529 nucleotidesGFF2 and GFF3 are separated by 14 nucleotidesGFF3 and GFF4 are separated by 179 nucleotides GFF1 - hypothetical protein, at 319 to 861 GFF1 GFF2 - Protein translocase subunit SecE, at 1,391 to 1,588 GFF2 GFF3 - Transcription termination/antitermination protein NusG, at 1,603 to 2,133 GFF3 GFF4 - 50S ribosomal protein L11, at 2,313 to 2,762 GFF4 Position (kb) 1 2Strain fitness (log2 ratio) -1 0 1at 0.536 kb on - strand, within GFF1at 0.612 kb on - strand, within GFF1at 0.664 kb on + strand, within GFF1at 0.665 kb on - strand, within GFF1at 0.706 kb on + strand, within GFF1at 0.860 kb on - strandat 1.214 kb on + strandat 1.214 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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536 - GFF1 0.40 -0.6
612 - GFF1 0.54 -0.1
664 + GFF1 0.64 -0.5
665 - GFF1 0.64 +0.8
706 + GFF1 0.71 +0.7
860 - +0.1
1,214 + -0.5
1,214 + +1.1

Or see this region's nucleotide sequence