Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF170 and GFF171 are separated by 134 nucleotides GFF171 and GFF172 are separated by 147 nucleotides GFF172 and GFF173 are separated by 80 nucleotides GFF173 and GFF174 are separated by 66 nucleotides
GFF170 - Acetolactate synthase isozyme 3 large subunit, at 178,759 to 180,519
GFF170
GFF171 - Acetolactate synthase small subunit, at 180,654 to 181,169
GFF171
GFF172 - Ketol-acid reductoisomerase (NADP(+)), at 181,317 to 182,336
GFF172
GFF173 - hypothetical protein, at 182,417 to 182,902
GFF173
GFF174 - 3-mercaptopropionate dioxygenase, at 182,969 to 183,616
GFF174
Position (kb) 181
182
183 Strain fitness (log2 ratio) -1
0
1
2 at 180.567 kb on + strand at 180.568 kb on - strand at 181.295 kb on + strand at 181.295 kb on + strand at 181.304 kb on + strand at 181.304 kb on + strand at 182.391 kb on + strand at 182.625 kb on + strand, within GFF173 at 182.625 kb on + strand, within GFF173 at 182.625 kb on + strand, within GFF173 at 182.626 kb on - strand, within GFF173 at 182.626 kb on - strand, within GFF173 at 182.651 kb on + strand, within GFF173 at 182.652 kb on - strand, within GFF173 at 182.938 kb on + strand at 182.939 kb on - strand at 183.034 kb on - strand, within GFF174
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 180,567 + -0.6 180,568 - -0.8 181,295 + +1.2 181,295 + +1.9 181,304 + +0.6 181,304 + -0.3 182,391 + -0.4 182,625 + GFF173 0.43 -0.8 182,625 + GFF173 0.43 +1.2 182,625 + GFF173 0.43 -1.0 182,626 - GFF173 0.43 -0.8 182,626 - GFF173 0.43 +0.3 182,651 + GFF173 0.48 +0.4 182,652 - GFF173 0.48 +1.0 182,938 + -1.5 182,939 - +1.2 183,034 - GFF174 0.10 -0.2
Or see this region's nucleotide sequence