Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03989 and MIT1002_03990 are separated by 280 nucleotides MIT1002_03990 and MIT1002_03991 are separated by 26 nucleotides MIT1002_03991 and MIT1002_03992 are separated by 71 nucleotides MIT1002_03992 and MIT1002_03993 are separated by 3 nucleotides
MIT1002_03989: MIT1002_03989 - Succinate semialdehyde dehydrogenase [NAD(P)+] Sad, at 4,476,116 to 4,477,504
_03989
MIT1002_03990: MIT1002_03990 - Non-canonical purine NTP phosphatase, at 4,477,785 to 4,478,330
_03990
MIT1002_03991: MIT1002_03991 - GMP/IMP nucleotidase YrfG, at 4,478,357 to 4,479,070
_03991
MIT1002_03992: MIT1002_03992 - ADP compounds hydrolase NudE, at 4,479,142 to 4,479,705
_03992
MIT1002_03993: MIT1002_03993 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 4,479,709 to 4,480,554
_03993
Position (kb) 4478
4479
4480 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4477.369 kb on - strand at 4477.510 kb on - strand at 4477.610 kb on - strand at 4477.616 kb on + strand at 4477.719 kb on + strand at 4477.748 kb on + strand at 4477.748 kb on + strand at 4477.768 kb on + strand at 4477.768 kb on + strand at 4477.776 kb on - strand at 4477.777 kb on + strand at 4477.785 kb on - strand at 4477.785 kb on - strand at 4477.785 kb on - strand at 4477.838 kb on - strand at 4477.866 kb on + strand, within MIT1002_03990 at 4477.954 kb on + strand, within MIT1002_03990 at 4477.957 kb on - strand, within MIT1002_03990 at 4477.973 kb on + strand, within MIT1002_03990 at 4478.005 kb on - strand, within MIT1002_03990 at 4478.026 kb on + strand, within MIT1002_03990 at 4478.049 kb on + strand, within MIT1002_03990 at 4478.076 kb on - strand, within MIT1002_03990 at 4478.086 kb on - strand, within MIT1002_03990 at 4478.088 kb on + strand, within MIT1002_03990 at 4478.114 kb on + strand, within MIT1002_03990 at 4478.114 kb on - strand, within MIT1002_03990 at 4478.125 kb on + strand, within MIT1002_03990 at 4478.140 kb on + strand, within MIT1002_03990 at 4478.198 kb on + strand, within MIT1002_03990 at 4478.211 kb on + strand, within MIT1002_03990 at 4478.216 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.221 kb on + strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.229 kb on - strand, within MIT1002_03990 at 4478.257 kb on - strand, within MIT1002_03990 at 4478.271 kb on - strand, within MIT1002_03990 at 4478.286 kb on - strand at 4478.379 kb on + strand at 4478.386 kb on - strand at 4478.433 kb on + strand, within MIT1002_03991 at 4478.433 kb on + strand, within MIT1002_03991 at 4478.469 kb on + strand, within MIT1002_03991 at 4478.513 kb on - strand, within MIT1002_03991 at 4478.592 kb on - strand, within MIT1002_03991 at 4478.651 kb on + strand, within MIT1002_03991 at 4478.651 kb on + strand, within MIT1002_03991 at 4478.679 kb on - strand, within MIT1002_03991 at 4478.697 kb on + strand, within MIT1002_03991 at 4478.979 kb on + strand, within MIT1002_03991 at 4478.987 kb on - strand, within MIT1002_03991 at 4479.015 kb on + strand at 4479.034 kb on - strand at 4479.088 kb on + strand at 4479.211 kb on + strand, within MIT1002_03992 at 4479.213 kb on + strand, within MIT1002_03992 at 4479.213 kb on + strand, within MIT1002_03992 at 4479.213 kb on + strand, within MIT1002_03992 at 4479.221 kb on - strand, within MIT1002_03992 at 4479.221 kb on - strand, within MIT1002_03992 at 4479.227 kb on + strand, within MIT1002_03992 at 4479.234 kb on - strand, within MIT1002_03992 at 4479.235 kb on - strand, within MIT1002_03992 at 4479.272 kb on - strand, within MIT1002_03992 at 4479.334 kb on + strand, within MIT1002_03992 at 4479.373 kb on + strand, within MIT1002_03992 at 4479.494 kb on + strand, within MIT1002_03992 at 4479.497 kb on - strand, within MIT1002_03992 at 4479.532 kb on + strand, within MIT1002_03992 at 4479.546 kb on + strand, within MIT1002_03992 at 4479.577 kb on + strand, within MIT1002_03992 at 4479.583 kb on - strand, within MIT1002_03992 at 4479.584 kb on - strand, within MIT1002_03992 at 4479.657 kb on + strand at 4479.659 kb on + strand at 4479.665 kb on - strand at 4479.724 kb on + strand at 4479.725 kb on + strand at 4479.735 kb on + strand at 4479.794 kb on + strand, within MIT1002_03993 at 4479.804 kb on + strand, within MIT1002_03993 at 4479.835 kb on + strand, within MIT1002_03993 at 4479.835 kb on + strand, within MIT1002_03993 at 4479.843 kb on - strand, within MIT1002_03993 at 4479.843 kb on - strand, within MIT1002_03993 at 4479.938 kb on + strand, within MIT1002_03993 at 4479.938 kb on + strand, within MIT1002_03993 at 4479.938 kb on + strand, within MIT1002_03993 at 4479.938 kb on + strand, within MIT1002_03993 at 4479.946 kb on - strand, within MIT1002_03993 at 4479.946 kb on - strand, within MIT1002_03993 at 4479.946 kb on - strand, within MIT1002_03993 at 4479.946 kb on - strand, within MIT1002_03993 at 4479.966 kb on + strand, within MIT1002_03993 at 4479.979 kb on - strand, within MIT1002_03993 at 4479.984 kb on + strand, within MIT1002_03993 at 4480.042 kb on - strand, within MIT1002_03993
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 4,477,369 - -0.5 4,477,510 - +1.3 4,477,610 - +0.3 4,477,616 + -0.2 4,477,719 + -0.9 4,477,748 + -1.2 4,477,748 + -0.8 4,477,768 + +0.7 4,477,768 + -1.4 4,477,776 - -1.4 4,477,777 + +0.9 4,477,785 - -0.1 4,477,785 - +2.0 4,477,785 - -0.9 4,477,838 - -3.4 4,477,866 + MIT1002_03990 0.15 +1.0 4,477,954 + MIT1002_03990 0.31 -0.8 4,477,957 - MIT1002_03990 0.32 +0.7 4,477,973 + MIT1002_03990 0.34 -0.8 4,478,005 - MIT1002_03990 0.40 -0.5 4,478,026 + MIT1002_03990 0.44 -0.1 4,478,049 + MIT1002_03990 0.48 -0.7 4,478,076 - MIT1002_03990 0.53 +0.3 4,478,086 - MIT1002_03990 0.55 -0.6 4,478,088 + MIT1002_03990 0.55 -0.7 4,478,114 + MIT1002_03990 0.60 -0.2 4,478,114 - MIT1002_03990 0.60 +0.7 4,478,125 + MIT1002_03990 0.62 -0.3 4,478,140 + MIT1002_03990 0.65 +0.6 4,478,198 + MIT1002_03990 0.76 +0.6 4,478,211 + MIT1002_03990 0.78 +0.3 4,478,216 + MIT1002_03990 0.79 -0.1 4,478,221 + MIT1002_03990 0.80 -0.2 4,478,221 + MIT1002_03990 0.80 -1.7 4,478,221 + MIT1002_03990 0.80 +2.2 4,478,221 + MIT1002_03990 0.80 -0.2 4,478,221 + MIT1002_03990 0.80 +0.1 4,478,221 + MIT1002_03990 0.80 -0.2 4,478,221 + MIT1002_03990 0.80 -0.2 4,478,221 + MIT1002_03990 0.80 +0.3 4,478,221 + MIT1002_03990 0.80 +0.9 4,478,221 + MIT1002_03990 0.80 -0.2 4,478,221 + MIT1002_03990 0.80 +0.0 4,478,221 + MIT1002_03990 0.80 -0.5 4,478,221 + MIT1002_03990 0.80 +1.1 4,478,221 + MIT1002_03990 0.80 -1.0 4,478,221 + MIT1002_03990 0.80 -0.0 4,478,229 - MIT1002_03990 0.81 -0.2 4,478,229 - MIT1002_03990 0.81 +0.7 4,478,229 - MIT1002_03990 0.81 -0.2 4,478,229 - MIT1002_03990 0.81 +0.1 4,478,229 - MIT1002_03990 0.81 +0.6 4,478,229 - MIT1002_03990 0.81 -0.6 4,478,229 - MIT1002_03990 0.81 +0.1 4,478,229 - MIT1002_03990 0.81 +0.6 4,478,229 - MIT1002_03990 0.81 +0.3 4,478,229 - MIT1002_03990 0.81 -1.1 4,478,229 - MIT1002_03990 0.81 -0.1 4,478,229 - MIT1002_03990 0.81 +2.5 4,478,229 - MIT1002_03990 0.81 +1.1 4,478,229 - MIT1002_03990 0.81 -0.4 4,478,257 - MIT1002_03990 0.86 -1.3 4,478,271 - MIT1002_03990 0.89 -0.4 4,478,286 - +0.3 4,478,379 + -0.9 4,478,386 - +1.7 4,478,433 + MIT1002_03991 0.11 +1.0 4,478,433 + MIT1002_03991 0.11 -0.4 4,478,469 + MIT1002_03991 0.16 +0.9 4,478,513 - MIT1002_03991 0.22 +1.5 4,478,592 - MIT1002_03991 0.33 +1.2 4,478,651 + MIT1002_03991 0.41 +1.3 4,478,651 + MIT1002_03991 0.41 +0.7 4,478,679 - MIT1002_03991 0.45 -0.2 4,478,697 + MIT1002_03991 0.48 +0.7 4,478,979 + MIT1002_03991 0.87 +0.4 4,478,987 - MIT1002_03991 0.88 -1.5 4,479,015 + +0.2 4,479,034 - -0.4 4,479,088 + -0.3 4,479,211 + MIT1002_03992 0.12 -0.8 4,479,213 + MIT1002_03992 0.13 +1.7 4,479,213 + MIT1002_03992 0.13 -0.3 4,479,213 + MIT1002_03992 0.13 +0.3 4,479,221 - MIT1002_03992 0.14 -1.4 4,479,221 - MIT1002_03992 0.14 -0.3 4,479,227 + MIT1002_03992 0.15 -0.9 4,479,234 - MIT1002_03992 0.16 -0.9 4,479,235 - MIT1002_03992 0.16 +0.8 4,479,272 - MIT1002_03992 0.23 -0.0 4,479,334 + MIT1002_03992 0.34 -0.3 4,479,373 + MIT1002_03992 0.41 +3.0 4,479,494 + MIT1002_03992 0.62 -0.4 4,479,497 - MIT1002_03992 0.63 +0.9 4,479,532 + MIT1002_03992 0.69 +0.1 4,479,546 + MIT1002_03992 0.72 -0.0 4,479,577 + MIT1002_03992 0.77 -0.1 4,479,583 - MIT1002_03992 0.78 +0.3 4,479,584 - MIT1002_03992 0.78 -0.9 4,479,657 + -0.5 4,479,659 + -2.2 4,479,665 - -1.9 4,479,724 + -1.5 4,479,725 + -0.9 4,479,735 + -0.4 4,479,794 + MIT1002_03993 0.10 -0.1 4,479,804 + MIT1002_03993 0.11 -1.0 4,479,835 + MIT1002_03993 0.15 -2.4 4,479,835 + MIT1002_03993 0.15 -0.4 4,479,843 - MIT1002_03993 0.16 -0.6 4,479,843 - MIT1002_03993 0.16 -1.0 4,479,938 + MIT1002_03993 0.27 -0.8 4,479,938 + MIT1002_03993 0.27 +0.0 4,479,938 + MIT1002_03993 0.27 +0.6 4,479,938 + MIT1002_03993 0.27 -2.1 4,479,946 - MIT1002_03993 0.28 -1.3 4,479,946 - MIT1002_03993 0.28 -1.5 4,479,946 - MIT1002_03993 0.28 -2.0 4,479,946 - MIT1002_03993 0.28 -1.5 4,479,966 + MIT1002_03993 0.30 -2.4 4,479,979 - MIT1002_03993 0.32 -2.1 4,479,984 + MIT1002_03993 0.33 -2.3 4,480,042 - MIT1002_03993 0.39 +1.0
Or see this region's nucleotide sequence