Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03737 and MIT1002_03738 are separated by 244 nucleotides MIT1002_03738 and MIT1002_03739 are separated by 14 nucleotides MIT1002_03739 and MIT1002_03740 are separated by 136 nucleotides MIT1002_03740 and MIT1002_03741 are separated by 4 nucleotides MIT1002_03741 and MIT1002_03742 are separated by 81 nucleotides MIT1002_03742 and MIT1002_03743 are separated by 167 nucleotides
MIT1002_03737: MIT1002_03737 - 50S ribosomal protein L11, at 4,180,113 to 4,180,541
_03737
MIT1002_03738: MIT1002_03738 - hypothetical protein, at 4,180,786 to 4,181,088
_03738
MIT1002_03739: MIT1002_03739 - hypothetical protein, at 4,181,103 to 4,181,297
_03739
MIT1002_03740: MIT1002_03740 - hypothetical protein, at 4,181,434 to 4,181,985
_03740
MIT1002_03741: MIT1002_03741 - Preprotein translocase subunit SecE, at 4,181,990 to 4,182,367
_03741
MIT1002_03742: MIT1002_03742 - tRNA-Trp, at 4,182,449 to 4,182,525
_03742
MIT1002_03743: MIT1002_03743 - Putative multidrug export ATP-binding/permease protein, at 4,182,693 to 4,184,534
_03743
Position (kb) 4181
4182 Strain fitness (log2 ratio) -1
0
1
2 at 4180.732 kb on + strand at 4180.746 kb on + strand at 4180.746 kb on + strand at 4180.746 kb on + strand at 4180.754 kb on - strand at 4180.754 kb on - strand at 4180.754 kb on - strand at 4180.754 kb on - strand at 4180.819 kb on - strand, within MIT1002_03738 at 4180.819 kb on - strand, within MIT1002_03738 at 4180.848 kb on - strand, within MIT1002_03738 at 4180.881 kb on - strand, within MIT1002_03738 at 4180.923 kb on + strand, within MIT1002_03738 at 4180.940 kb on - strand, within MIT1002_03738 at 4180.948 kb on - strand, within MIT1002_03738 at 4180.980 kb on + strand, within MIT1002_03738 at 4180.995 kb on - strand, within MIT1002_03738 at 4181.007 kb on + strand, within MIT1002_03738 at 4181.021 kb on + strand, within MIT1002_03738 at 4181.064 kb on - strand at 4181.429 kb on - strand at 4181.431 kb on + strand at 4181.439 kb on - strand at 4181.439 kb on - strand at 4181.441 kb on + strand at 4182.463 kb on - strand, within MIT1002_03742 at 4182.464 kb on - strand, within MIT1002_03742 at 4182.480 kb on - strand, within MIT1002_03742 at 4182.529 kb on - strand at 4182.529 kb on - strand at 4182.616 kb on + strand at 4182.687 kb on - strand at 4182.727 kb on + strand at 4182.727 kb on + strand at 4182.727 kb on + strand at 4182.727 kb on + strand at 4182.727 kb on + strand at 4182.727 kb on + strand at 4182.735 kb on - strand at 4182.735 kb on - strand at 4182.735 kb on - strand at 4182.735 kb on - strand at 4182.735 kb on - strand at 4182.735 kb on - strand at 4182.775 kb on + strand at 4182.782 kb on + strand at 4182.790 kb on + strand at 4182.796 kb on + strand at 4182.865 kb on + strand at 4182.902 kb on - strand, within MIT1002_03743 at 4182.902 kb on - strand, within MIT1002_03743 at 4182.931 kb on + strand, within MIT1002_03743 at 4182.934 kb on - strand, within MIT1002_03743 at 4182.945 kb on - strand, within MIT1002_03743 at 4182.981 kb on + strand, within MIT1002_03743
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 4,180,732 + +1.1 4,180,746 + +0.1 4,180,746 + -0.2 4,180,746 + -0.0 4,180,754 - +0.7 4,180,754 - -0.1 4,180,754 - -0.9 4,180,754 - +0.0 4,180,819 - MIT1002_03738 0.11 +0.0 4,180,819 - MIT1002_03738 0.11 +0.3 4,180,848 - MIT1002_03738 0.20 -1.4 4,180,881 - MIT1002_03738 0.31 -0.1 4,180,923 + MIT1002_03738 0.45 +1.5 4,180,940 - MIT1002_03738 0.51 +0.8 4,180,948 - MIT1002_03738 0.53 +0.1 4,180,980 + MIT1002_03738 0.64 +0.3 4,180,995 - MIT1002_03738 0.69 +0.7 4,181,007 + MIT1002_03738 0.73 +0.6 4,181,021 + MIT1002_03738 0.78 +1.7 4,181,064 - -0.9 4,181,429 - +0.4 4,181,431 + -1.0 4,181,439 - +0.3 4,181,439 - +0.0 4,181,441 + +1.2 4,182,463 - MIT1002_03742 0.18 -0.4 4,182,464 - MIT1002_03742 0.19 +0.4 4,182,480 - MIT1002_03742 0.40 -0.4 4,182,529 - +0.7 4,182,529 - -0.6 4,182,616 + -1.0 4,182,687 - +0.2 4,182,727 + -0.5 4,182,727 + +0.7 4,182,727 + +0.5 4,182,727 + -1.4 4,182,727 + +1.8 4,182,727 + +0.2 4,182,735 - +0.7 4,182,735 - -0.2 4,182,735 - +0.3 4,182,735 - +0.7 4,182,735 - -0.4 4,182,735 - -0.1 4,182,775 + -0.8 4,182,782 + -0.5 4,182,790 + +0.8 4,182,796 + +1.6 4,182,865 + -1.7 4,182,902 - MIT1002_03743 0.11 +2.0 4,182,902 - MIT1002_03743 0.11 -0.6 4,182,931 + MIT1002_03743 0.13 -0.7 4,182,934 - MIT1002_03743 0.13 -0.5 4,182,945 - MIT1002_03743 0.14 +0.0 4,182,981 + MIT1002_03743 0.16 -0.3
Or see this region's nucleotide sequence