Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03717 and MIT1002_03718 are separated by 18 nucleotides MIT1002_03718 and MIT1002_03719 are separated by 2 nucleotides MIT1002_03719 and MIT1002_03720 overlap by 1 nucleotides MIT1002_03720 and MIT1002_03721 overlap by 4 nucleotides MIT1002_03721 and MIT1002_03722 are separated by 102 nucleotides
MIT1002_03717: MIT1002_03717 - Gram-negative bacterial tonB protein, at 4,151,693 to 4,152,313
_03717
MIT1002_03718: MIT1002_03718 - biopolymer transport protein ExbD, at 4,152,332 to 4,152,739
_03718
MIT1002_03719: MIT1002_03719 - biopolymer transport protein ExbB, at 4,152,742 to 4,153,128
_03719
MIT1002_03720: MIT1002_03720 - Biopolymer transport protein ExbB, at 4,153,128 to 4,154,504
_03720
MIT1002_03721: MIT1002_03721 - hypothetical protein, at 4,154,501 to 4,155,277
_03721
MIT1002_03722: MIT1002_03722 - Ferric enterobactin receptor precursor, at 4,155,380 to 4,157,671
_03722
Position (kb)
4153
4154
4155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4152.204 kb on + strand, within MIT1002_03717 at 4152.205 kb on + strand, within MIT1002_03717 at 4152.210 kb on + strand, within MIT1002_03717 at 4152.223 kb on - strand, within MIT1002_03717 at 4152.260 kb on - strand at 4152.281 kb on + strand at 4152.294 kb on + strand at 4152.302 kb on - strand at 4152.302 kb on - strand at 4152.337 kb on + strand at 4152.512 kb on + strand, within MIT1002_03718 at 4152.512 kb on + strand, within MIT1002_03718 at 4152.512 kb on + strand, within MIT1002_03718 at 4152.512 kb on + strand, within MIT1002_03718 at 4152.512 kb on + strand, within MIT1002_03718 at 4152.512 kb on + strand, within MIT1002_03718 at 4152.512 kb on + strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.520 kb on - strand, within MIT1002_03718 at 4152.529 kb on - strand, within MIT1002_03718 at 4152.579 kb on + strand, within MIT1002_03718 at 4152.683 kb on + strand, within MIT1002_03718 at 4152.740 kb on + strand at 4152.748 kb on - strand at 4152.748 kb on - strand at 4152.748 kb on - strand at 4152.750 kb on + strand at 4152.802 kb on + strand, within MIT1002_03719 at 4152.816 kb on + strand, within MIT1002_03719 at 4152.819 kb on - strand, within MIT1002_03719 at 4152.825 kb on - strand, within MIT1002_03719 at 4152.874 kb on + strand, within MIT1002_03719 at 4152.956 kb on + strand, within MIT1002_03719 at 4152.964 kb on - strand, within MIT1002_03719 at 4152.974 kb on - strand, within MIT1002_03719 at 4153.015 kb on + strand, within MIT1002_03719 at 4153.029 kb on - strand, within MIT1002_03719 at 4153.086 kb on + strand, within MIT1002_03719 at 4153.091 kb on + strand at 4153.091 kb on + strand at 4153.091 kb on + strand at 4153.094 kb on - strand at 4153.099 kb on - strand at 4153.099 kb on - strand at 4153.145 kb on + strand at 4153.192 kb on - strand at 4153.299 kb on + strand, within MIT1002_03720 at 4153.299 kb on + strand, within MIT1002_03720 at 4153.300 kb on - strand, within MIT1002_03720 at 4153.307 kb on - strand, within MIT1002_03720 at 4153.314 kb on + strand, within MIT1002_03720 at 4153.322 kb on - strand, within MIT1002_03720 at 4153.394 kb on - strand, within MIT1002_03720 at 4153.498 kb on + strand, within MIT1002_03720 at 4153.559 kb on - strand, within MIT1002_03720 at 4153.586 kb on - strand, within MIT1002_03720 at 4153.589 kb on + strand, within MIT1002_03720 at 4153.589 kb on + strand, within MIT1002_03720 at 4153.589 kb on + strand, within MIT1002_03720 at 4153.597 kb on - strand, within MIT1002_03720 at 4153.597 kb on - strand, within MIT1002_03720 at 4153.597 kb on - strand, within MIT1002_03720 at 4153.611 kb on + strand, within MIT1002_03720 at 4153.611 kb on + strand, within MIT1002_03720 at 4153.618 kb on + strand, within MIT1002_03720 at 4153.669 kb on + strand, within MIT1002_03720 at 4153.669 kb on + strand, within MIT1002_03720 at 4153.669 kb on + strand, within MIT1002_03720 at 4153.677 kb on - strand, within MIT1002_03720 at 4153.677 kb on - strand, within MIT1002_03720 at 4153.677 kb on - strand, within MIT1002_03720 at 4153.689 kb on - strand, within MIT1002_03720 at 4153.718 kb on + strand, within MIT1002_03720 at 4153.730 kb on - strand, within MIT1002_03720 at 4153.732 kb on - strand, within MIT1002_03720 at 4153.759 kb on - strand, within MIT1002_03720 at 4153.768 kb on - strand, within MIT1002_03720 at 4153.856 kb on - strand, within MIT1002_03720 at 4153.900 kb on - strand, within MIT1002_03720 at 4153.900 kb on - strand, within MIT1002_03720 at 4153.915 kb on - strand, within MIT1002_03720 at 4154.003 kb on - strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.112 kb on + strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.120 kb on - strand, within MIT1002_03720 at 4154.122 kb on + strand, within MIT1002_03720 at 4154.122 kb on + strand, within MIT1002_03720 at 4154.130 kb on - strand, within MIT1002_03720 at 4154.201 kb on + strand, within MIT1002_03720 at 4154.235 kb on - strand, within MIT1002_03720 at 4154.261 kb on + strand, within MIT1002_03720 at 4154.284 kb on - strand, within MIT1002_03720 at 4154.284 kb on - strand, within MIT1002_03720 at 4154.384 kb on + strand at 4154.412 kb on + strand at 4154.415 kb on - strand at 4154.429 kb on + strand at 4154.429 kb on + strand at 4154.455 kb on + strand at 4154.455 kb on + strand at 4154.475 kb on - strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.514 kb on + strand at 4154.522 kb on - strand at 4154.522 kb on - strand at 4154.522 kb on - strand at 4154.532 kb on - strand at 4154.579 kb on + strand, within MIT1002_03721 at 4154.655 kb on + strand, within MIT1002_03721 at 4154.708 kb on + strand, within MIT1002_03721 at 4154.792 kb on + strand, within MIT1002_03721 at 4154.822 kb on - strand, within MIT1002_03721 at 4154.832 kb on - strand, within MIT1002_03721 at 4154.902 kb on + strand, within MIT1002_03721 at 4154.910 kb on - strand, within MIT1002_03721 at 4154.910 kb on - strand, within MIT1002_03721 at 4154.929 kb on - strand, within MIT1002_03721 at 4154.936 kb on - strand, within MIT1002_03721 at 4154.946 kb on - strand, within MIT1002_03721 at 4154.949 kb on + strand, within MIT1002_03721 at 4154.955 kb on - strand, within MIT1002_03721 at 4154.957 kb on - strand, within MIT1002_03721 at 4154.960 kb on - strand, within MIT1002_03721 at 4154.962 kb on - strand, within MIT1002_03721 at 4155.035 kb on - strand, within MIT1002_03721 at 4155.069 kb on + strand, within MIT1002_03721 at 4155.069 kb on + strand, within MIT1002_03721 at 4155.077 kb on - strand, within MIT1002_03721 at 4155.077 kb on - strand, within MIT1002_03721 at 4155.085 kb on - strand, within MIT1002_03721 at 4155.231 kb on + strand at 4155.394 kb on - strand at 4155.407 kb on - strand at 4155.413 kb on + strand at 4155.440 kb on - strand at 4155.452 kb on - strand at 4155.454 kb on + strand at 4155.470 kb on - strand at 4155.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 4,152,204 + MIT1002_03717 0.82 -0.5 4,152,205 + MIT1002_03717 0.82 -3.6 4,152,210 + MIT1002_03717 0.83 +0.7 4,152,223 - MIT1002_03717 0.85 +0.2 4,152,260 - -0.0 4,152,281 + -0.7 4,152,294 + +0.8 4,152,302 - -1.5 4,152,302 - -0.1 4,152,337 + -0.1 4,152,512 + MIT1002_03718 0.44 +0.9 4,152,512 + MIT1002_03718 0.44 -1.1 4,152,512 + MIT1002_03718 0.44 +2.3 4,152,512 + MIT1002_03718 0.44 +0.1 4,152,512 + MIT1002_03718 0.44 +0.3 4,152,512 + MIT1002_03718 0.44 -0.6 4,152,512 + MIT1002_03718 0.44 -1.1 4,152,520 - MIT1002_03718 0.46 +1.2 4,152,520 - MIT1002_03718 0.46 -1.1 4,152,520 - MIT1002_03718 0.46 +1.0 4,152,520 - MIT1002_03718 0.46 -0.3 4,152,520 - MIT1002_03718 0.46 -1.6 4,152,520 - MIT1002_03718 0.46 -0.2 4,152,520 - MIT1002_03718 0.46 +0.1 4,152,529 - MIT1002_03718 0.48 -2.0 4,152,579 + MIT1002_03718 0.61 +0.7 4,152,683 + MIT1002_03718 0.86 +1.5 4,152,740 + +2.2 4,152,748 - -0.9 4,152,748 - +2.0 4,152,748 - +1.8 4,152,750 + -0.3 4,152,802 + MIT1002_03719 0.16 +0.1 4,152,816 + MIT1002_03719 0.19 -0.9 4,152,819 - MIT1002_03719 0.20 +0.1 4,152,825 - MIT1002_03719 0.21 -0.6 4,152,874 + MIT1002_03719 0.34 -0.7 4,152,956 + MIT1002_03719 0.55 +0.5 4,152,964 - MIT1002_03719 0.57 +0.0 4,152,974 - MIT1002_03719 0.60 +0.6 4,153,015 + MIT1002_03719 0.71 -0.1 4,153,029 - MIT1002_03719 0.74 +1.0 4,153,086 + MIT1002_03719 0.89 +0.5 4,153,091 + +0.4 4,153,091 + -1.6 4,153,091 + -1.2 4,153,094 - -1.3 4,153,099 - +0.2 4,153,099 - +0.5 4,153,145 + -0.1 4,153,192 - -0.4 4,153,299 + MIT1002_03720 0.12 +0.6 4,153,299 + MIT1002_03720 0.12 -0.1 4,153,300 - MIT1002_03720 0.12 -1.1 4,153,307 - MIT1002_03720 0.13 -0.4 4,153,314 + MIT1002_03720 0.14 -0.8 4,153,322 - MIT1002_03720 0.14 +1.2 4,153,394 - MIT1002_03720 0.19 -0.9 4,153,498 + MIT1002_03720 0.27 -1.7 4,153,559 - MIT1002_03720 0.31 -1.1 4,153,586 - MIT1002_03720 0.33 +0.7 4,153,589 + MIT1002_03720 0.33 -0.4 4,153,589 + MIT1002_03720 0.33 -0.8 4,153,589 + MIT1002_03720 0.33 -2.3 4,153,597 - MIT1002_03720 0.34 -0.7 4,153,597 - MIT1002_03720 0.34 +0.2 4,153,597 - MIT1002_03720 0.34 -1.0 4,153,611 + MIT1002_03720 0.35 -0.5 4,153,611 + MIT1002_03720 0.35 +0.2 4,153,618 + MIT1002_03720 0.36 -2.0 4,153,669 + MIT1002_03720 0.39 -0.4 4,153,669 + MIT1002_03720 0.39 -0.6 4,153,669 + MIT1002_03720 0.39 -0.1 4,153,677 - MIT1002_03720 0.40 -0.6 4,153,677 - MIT1002_03720 0.40 +0.5 4,153,677 - MIT1002_03720 0.40 -0.1 4,153,689 - MIT1002_03720 0.41 +0.2 4,153,718 + MIT1002_03720 0.43 -0.2 4,153,730 - MIT1002_03720 0.44 -0.8 4,153,732 - MIT1002_03720 0.44 +0.6 4,153,759 - MIT1002_03720 0.46 +0.1 4,153,768 - MIT1002_03720 0.46 -0.1 4,153,856 - MIT1002_03720 0.53 -1.5 4,153,900 - MIT1002_03720 0.56 -2.1 4,153,900 - MIT1002_03720 0.56 -1.2 4,153,915 - MIT1002_03720 0.57 -0.3 4,154,003 - MIT1002_03720 0.64 -0.6 4,154,112 + MIT1002_03720 0.71 +0.8 4,154,112 + MIT1002_03720 0.71 -0.9 4,154,112 + MIT1002_03720 0.71 -0.1 4,154,112 + MIT1002_03720 0.71 -1.0 4,154,112 + MIT1002_03720 0.71 +0.1 4,154,112 + MIT1002_03720 0.71 +0.2 4,154,112 + MIT1002_03720 0.71 +0.8 4,154,112 + MIT1002_03720 0.71 +1.1 4,154,120 - MIT1002_03720 0.72 -0.3 4,154,120 - MIT1002_03720 0.72 -0.6 4,154,120 - MIT1002_03720 0.72 +0.1 4,154,120 - MIT1002_03720 0.72 +0.4 4,154,120 - MIT1002_03720 0.72 +0.6 4,154,120 - MIT1002_03720 0.72 -0.5 4,154,120 - MIT1002_03720 0.72 -1.6 4,154,120 - MIT1002_03720 0.72 -0.0 4,154,122 + MIT1002_03720 0.72 -1.1 4,154,122 + MIT1002_03720 0.72 -1.9 4,154,130 - MIT1002_03720 0.73 -1.9 4,154,201 + MIT1002_03720 0.78 +0.7 4,154,235 - MIT1002_03720 0.80 -0.0 4,154,261 + MIT1002_03720 0.82 -0.3 4,154,284 - MIT1002_03720 0.84 -0.4 4,154,284 - MIT1002_03720 0.84 -0.8 4,154,384 + -1.0 4,154,412 + +1.1 4,154,415 - -0.3 4,154,429 + +0.1 4,154,429 + +1.4 4,154,455 + +1.0 4,154,455 + +0.4 4,154,475 - +0.8 4,154,514 + +0.4 4,154,514 + -1.1 4,154,514 + -0.9 4,154,514 + -0.4 4,154,514 + -0.6 4,154,514 + +0.1 4,154,514 + -1.2 4,154,514 + +1.5 4,154,522 - -0.7 4,154,522 - -0.2 4,154,522 - +2.3 4,154,532 - -0.3 4,154,579 + MIT1002_03721 0.10 +0.6 4,154,655 + MIT1002_03721 0.20 +0.1 4,154,708 + MIT1002_03721 0.27 +0.2 4,154,792 + MIT1002_03721 0.37 +0.4 4,154,822 - MIT1002_03721 0.41 +0.3 4,154,832 - MIT1002_03721 0.43 -0.0 4,154,902 + MIT1002_03721 0.52 +2.8 4,154,910 - MIT1002_03721 0.53 -0.2 4,154,910 - MIT1002_03721 0.53 +2.7 4,154,929 - MIT1002_03721 0.55 -0.3 4,154,936 - MIT1002_03721 0.56 +0.7 4,154,946 - MIT1002_03721 0.57 -0.2 4,154,949 + MIT1002_03721 0.58 -0.1 4,154,955 - MIT1002_03721 0.58 +1.7 4,154,957 - MIT1002_03721 0.59 -1.0 4,154,960 - MIT1002_03721 0.59 -0.3 4,154,962 - MIT1002_03721 0.59 -0.3 4,155,035 - MIT1002_03721 0.69 +1.0 4,155,069 + MIT1002_03721 0.73 -0.1 4,155,069 + MIT1002_03721 0.73 -0.4 4,155,077 - MIT1002_03721 0.74 -2.0 4,155,077 - MIT1002_03721 0.74 +0.9 4,155,085 - MIT1002_03721 0.75 +2.1 4,155,231 + +1.4 4,155,394 - -1.0 4,155,407 - -0.6 4,155,413 + -2.5 4,155,440 - +0.5 4,155,452 - +0.3 4,155,454 + -0.4 4,155,470 - -0.9 4,155,477 - -0.7
Or see this region's nucleotide sequence