Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02562 and MIT1002_02563 are separated by 64 nucleotides MIT1002_02563 and MIT1002_02564 overlap by 4 nucleotides MIT1002_02564 and MIT1002_02565 are separated by 63 nucleotides MIT1002_02565 and MIT1002_02566 are separated by 141 nucleotides
MIT1002_02562: MIT1002_02562 - hypothetical protein, at 2,860,375 to 2,861,085
_02562
MIT1002_02563: MIT1002_02563 - Long-chain acyl-CoA thioesterase FadM, at 2,861,150 to 2,861,599
_02563
MIT1002_02564: MIT1002_02564 - clan AA aspartic protease, at 2,861,596 to 2,862,831
_02564
MIT1002_02565: MIT1002_02565 - HopJ type III effector protein, at 2,862,895 to 2,863,254
_02565
MIT1002_02566: MIT1002_02566 - 2,4-dienoyl-CoA reductase [NADPH], at 2,863,396 to 2,865,438
_02566
Position (kb)
2861
2862
2863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2860.655 kb on + strand, within MIT1002_02562 at 2860.711 kb on + strand, within MIT1002_02562 at 2860.719 kb on - strand, within MIT1002_02562 at 2860.783 kb on - strand, within MIT1002_02562 at 2860.783 kb on - strand, within MIT1002_02562 at 2860.868 kb on + strand, within MIT1002_02562 at 2860.923 kb on - strand, within MIT1002_02562 at 2860.985 kb on + strand, within MIT1002_02562 at 2860.985 kb on + strand, within MIT1002_02562 at 2860.991 kb on - strand, within MIT1002_02562 at 2860.993 kb on - strand, within MIT1002_02562 at 2861.044 kb on + strand at 2861.052 kb on - strand at 2861.061 kb on + strand at 2861.087 kb on - strand at 2861.095 kb on - strand at 2861.113 kb on + strand at 2861.113 kb on + strand at 2861.113 kb on + strand at 2861.121 kb on - strand at 2861.155 kb on - strand at 2861.157 kb on + strand at 2861.165 kb on - strand at 2861.165 kb on - strand at 2861.185 kb on + strand at 2861.192 kb on - strand at 2861.200 kb on - strand, within MIT1002_02563 at 2861.239 kb on + strand, within MIT1002_02563 at 2861.274 kb on + strand, within MIT1002_02563 at 2861.282 kb on - strand, within MIT1002_02563 at 2861.287 kb on - strand, within MIT1002_02563 at 2861.297 kb on - strand, within MIT1002_02563 at 2861.374 kb on + strand, within MIT1002_02563 at 2861.376 kb on + strand, within MIT1002_02563 at 2861.385 kb on + strand, within MIT1002_02563 at 2861.415 kb on + strand, within MIT1002_02563 at 2861.423 kb on - strand, within MIT1002_02563 at 2861.517 kb on + strand, within MIT1002_02563 at 2861.520 kb on - strand, within MIT1002_02563 at 2861.532 kb on - strand, within MIT1002_02563 at 2861.630 kb on + strand at 2861.636 kb on - strand at 2861.672 kb on + strand at 2861.672 kb on + strand at 2861.712 kb on - strand at 2861.714 kb on + strand at 2861.714 kb on + strand at 2861.769 kb on - strand, within MIT1002_02564 at 2861.837 kb on - strand, within MIT1002_02564 at 2861.885 kb on + strand, within MIT1002_02564 at 2861.929 kb on + strand, within MIT1002_02564 at 2862.023 kb on + strand, within MIT1002_02564 at 2862.026 kb on - strand, within MIT1002_02564 at 2862.031 kb on - strand, within MIT1002_02564 at 2862.032 kb on - strand, within MIT1002_02564 at 2862.152 kb on - strand, within MIT1002_02564 at 2862.238 kb on + strand, within MIT1002_02564 at 2862.248 kb on - strand, within MIT1002_02564 at 2862.264 kb on + strand, within MIT1002_02564 at 2862.272 kb on - strand, within MIT1002_02564 at 2862.272 kb on - strand, within MIT1002_02564 at 2862.384 kb on + strand, within MIT1002_02564 at 2862.413 kb on - strand, within MIT1002_02564 at 2862.440 kb on - strand, within MIT1002_02564 at 2862.457 kb on + strand, within MIT1002_02564 at 2862.465 kb on - strand, within MIT1002_02564 at 2862.465 kb on - strand, within MIT1002_02564 at 2862.493 kb on - strand, within MIT1002_02564 at 2862.626 kb on - strand, within MIT1002_02564 at 2862.626 kb on - strand, within MIT1002_02564 at 2862.698 kb on - strand, within MIT1002_02564 at 2862.764 kb on + strand at 2862.797 kb on + strand at 2862.818 kb on - strand at 2862.826 kb on + strand at 2862.826 kb on + strand at 2862.840 kb on + strand at 2862.847 kb on + strand at 2862.848 kb on - strand at 2862.855 kb on - strand at 2862.862 kb on - strand at 2863.064 kb on - strand, within MIT1002_02565 at 2863.083 kb on + strand, within MIT1002_02565 at 2863.091 kb on - strand, within MIT1002_02565 at 2863.094 kb on + strand, within MIT1002_02565 at 2863.140 kb on - strand, within MIT1002_02565 at 2863.171 kb on - strand, within MIT1002_02565 at 2863.239 kb on - strand at 2863.243 kb on + strand at 2863.251 kb on - strand at 2863.287 kb on + strand at 2863.288 kb on + strand at 2863.295 kb on - strand at 2863.295 kb on - strand at 2863.296 kb on - strand at 2863.296 kb on - strand at 2863.314 kb on - strand at 2863.322 kb on + strand at 2863.369 kb on + strand at 2863.500 kb on - strand at 2863.500 kb on - strand at 2863.562 kb on + strand at 2863.588 kb on + strand at 2863.596 kb on - strand at 2863.643 kb on + strand, within MIT1002_02566 at 2863.656 kb on + strand, within MIT1002_02566 at 2863.665 kb on + strand, within MIT1002_02566 at 2863.668 kb on + strand, within MIT1002_02566 at 2863.687 kb on + strand, within MIT1002_02566 at 2863.687 kb on + strand, within MIT1002_02566 at 2863.687 kb on + strand, within MIT1002_02566 at 2863.695 kb on - strand, within MIT1002_02566 at 2863.695 kb on - strand, within MIT1002_02566 at 2863.695 kb on - strand, within MIT1002_02566 at 2863.756 kb on - strand, within MIT1002_02566 at 2863.756 kb on - strand, within MIT1002_02566 at 2863.788 kb on + strand, within MIT1002_02566 at 2863.803 kb on - strand, within MIT1002_02566
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 2,860,655 + MIT1002_02562 0.39 +1.3 2,860,711 + MIT1002_02562 0.47 -0.4 2,860,719 - MIT1002_02562 0.48 +0.2 2,860,783 - MIT1002_02562 0.57 +0.8 2,860,783 - MIT1002_02562 0.57 +1.3 2,860,868 + MIT1002_02562 0.69 -1.5 2,860,923 - MIT1002_02562 0.77 +0.2 2,860,985 + MIT1002_02562 0.86 -1.3 2,860,985 + MIT1002_02562 0.86 +2.0 2,860,991 - MIT1002_02562 0.87 -0.6 2,860,993 - MIT1002_02562 0.87 -0.1 2,861,044 + +0.3 2,861,052 - +0.6 2,861,061 + -0.0 2,861,087 - +0.6 2,861,095 - -1.8 2,861,113 + +0.2 2,861,113 + -0.6 2,861,113 + -0.2 2,861,121 - -2.8 2,861,155 - -1.4 2,861,157 + -1.2 2,861,165 - +0.7 2,861,165 - -0.0 2,861,185 + +0.3 2,861,192 - +0.0 2,861,200 - MIT1002_02563 0.11 +1.3 2,861,239 + MIT1002_02563 0.20 -0.5 2,861,274 + MIT1002_02563 0.28 +0.2 2,861,282 - MIT1002_02563 0.29 -1.2 2,861,287 - MIT1002_02563 0.30 -0.1 2,861,297 - MIT1002_02563 0.33 +0.0 2,861,374 + MIT1002_02563 0.50 +0.2 2,861,376 + MIT1002_02563 0.50 -0.5 2,861,385 + MIT1002_02563 0.52 +0.4 2,861,415 + MIT1002_02563 0.59 -0.1 2,861,423 - MIT1002_02563 0.61 -0.5 2,861,517 + MIT1002_02563 0.82 -1.4 2,861,520 - MIT1002_02563 0.82 +0.6 2,861,532 - MIT1002_02563 0.85 -1.0 2,861,630 + +0.2 2,861,636 - +1.2 2,861,672 + +0.1 2,861,672 + -1.3 2,861,712 - -0.5 2,861,714 + +1.1 2,861,714 + -0.3 2,861,769 - MIT1002_02564 0.14 -0.2 2,861,837 - MIT1002_02564 0.19 +0.0 2,861,885 + MIT1002_02564 0.23 -0.3 2,861,929 + MIT1002_02564 0.27 +0.6 2,862,023 + MIT1002_02564 0.35 +0.0 2,862,026 - MIT1002_02564 0.35 -1.4 2,862,031 - MIT1002_02564 0.35 +0.3 2,862,032 - MIT1002_02564 0.35 -0.2 2,862,152 - MIT1002_02564 0.45 +0.2 2,862,238 + MIT1002_02564 0.52 +0.1 2,862,248 - MIT1002_02564 0.53 -0.2 2,862,264 + MIT1002_02564 0.54 +0.6 2,862,272 - MIT1002_02564 0.55 +0.0 2,862,272 - MIT1002_02564 0.55 -1.4 2,862,384 + MIT1002_02564 0.64 +0.8 2,862,413 - MIT1002_02564 0.66 -1.0 2,862,440 - MIT1002_02564 0.68 +0.3 2,862,457 + MIT1002_02564 0.70 -0.0 2,862,465 - MIT1002_02564 0.70 +0.6 2,862,465 - MIT1002_02564 0.70 +1.4 2,862,493 - MIT1002_02564 0.73 +0.2 2,862,626 - MIT1002_02564 0.83 -0.7 2,862,626 - MIT1002_02564 0.83 -0.8 2,862,698 - MIT1002_02564 0.89 +1.3 2,862,764 + -0.7 2,862,797 + -0.8 2,862,818 - +1.2 2,862,826 + +0.2 2,862,826 + -0.8 2,862,840 + -0.2 2,862,847 + +0.5 2,862,848 - +0.0 2,862,855 - +0.2 2,862,862 - -1.8 2,863,064 - MIT1002_02565 0.47 -0.8 2,863,083 + MIT1002_02565 0.52 -1.6 2,863,091 - MIT1002_02565 0.54 -0.4 2,863,094 + MIT1002_02565 0.55 -0.8 2,863,140 - MIT1002_02565 0.68 +0.2 2,863,171 - MIT1002_02565 0.77 +1.6 2,863,239 - -1.5 2,863,243 + -0.7 2,863,251 - -0.6 2,863,287 + +1.4 2,863,288 + -0.3 2,863,295 - -2.8 2,863,295 - +1.2 2,863,296 - -0.5 2,863,296 - +0.2 2,863,314 - +1.1 2,863,322 + +1.2 2,863,369 + +1.7 2,863,500 - -1.1 2,863,500 - -0.1 2,863,562 + -1.3 2,863,588 + -0.0 2,863,596 - -1.5 2,863,643 + MIT1002_02566 0.12 +0.0 2,863,656 + MIT1002_02566 0.13 +0.6 2,863,665 + MIT1002_02566 0.13 +1.6 2,863,668 + MIT1002_02566 0.13 +0.2 2,863,687 + MIT1002_02566 0.14 -0.2 2,863,687 + MIT1002_02566 0.14 +0.4 2,863,687 + MIT1002_02566 0.14 +0.7 2,863,695 - MIT1002_02566 0.15 -0.4 2,863,695 - MIT1002_02566 0.15 -0.9 2,863,695 - MIT1002_02566 0.15 +0.9 2,863,756 - MIT1002_02566 0.18 +0.6 2,863,756 - MIT1002_02566 0.18 -3.1 2,863,788 + MIT1002_02566 0.19 +0.4 2,863,803 - MIT1002_02566 0.20 -0.1
Or see this region's nucleotide sequence